Recombinant human Tissue Plasminogen Activator protein, Two Chain (Active) is a Human Full Length protein, in the 36 to 562 aa range, expressed in CHO, with >95% purity and suitable for SDS-PAGE, FuncS.
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- Research and practice in thrombosis and haemostasis 8:1022912024Fluorescent peptide for detecting factor XIIIa activity and fibrin in whole blood clots forming under flow.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Liu,Jennifer Crossen,Timothy J Stalker,Scott L DiamondPubMed 38222077
- International journal of molecular sciences 23:20221,2,3-Triazole Derivatives as Novel Antifibrinolytic Drugs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOriol Bosch-Sanz,Yvette Rabadà,Xevi Biarnés,Javier Pedreño,Luis Caveda,Mercedes Balcells,Jordi Martorell,David Sánchez-GarcíaPubMed 36499270
- Science advances 8:eabn26272022Reduced platelet forces underlie impaired hemostasis in mouse models of -related disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuliane Baumann,Laura Sachs,Oliver Otto,Ingmar Schoen,Peter Nestler,Carlo Zaninetti,Martin Kenny,Ruth Kranz,Hendrik von Eysmondt,Johanna Rodriguez,Tilman E Schäffer,Zoltan Nagy,Andreas Greinacher,Raghavendra Palankar,Markus BenderPubMed 35584211
- Frontiers in cell and developmental biology 8:5643512020Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Is a Negative Regulator of Oligodendrocyte Progenitor Cell Differentiation in the Adult Mouse Brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLoic Auderset,Kimberley A Pitman,Carlie L Cullen,Renee E Pepper,Bruce V Taylor,Lisa Foa,Kaylene M YoungPubMed 33282858
- OncoTargets and therapy 10:2077-20852017Expression and activity of the urokinase plasminogen activator system in canine primary brain tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn H Rossmeisl et. al.PubMed 28442916
- Theranostics 5:267-762015Serum fucosylated prostate-specific antigen (PSA) improves the differentiation of aggressive from non-aggressive prostate cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Kay Li et. al.PubMed 25553114
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- CHO cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
S Y Q V I C R D E K T Q M I Y Q Q H Q S W L R P V L R S N R V E Y C W C N S G R A Q C H S V P V K S C S E P R C F N G G T C Q Q A L Y F S D F V C Q C P E G F A G K C C E I D T R A T C Y E D Q G I S Y R G T W S T A E S G A E C T N W N S S A L A Q K P Y S G R R P D A I R L G L G N H N Y C R N P D R D S K P W C Y V F K A G K Y S S E F C S T P A C S E G N S D C Y F G N G S A Y R G T H S L T E S G A S C L P W N S M I L I G K V Y T A Q N P S A Q A L G L G K H N Y C R N P D G D A K P W C H V L K N R R L T W E Y C D V P S C S T C G L R Q Y S Q P Q F R I K G G L F A D I A S H P W Q A A I F A K H R R S P G E R F L C G G I L I S S C W I L S A A H C F Q E R F P P H H L T V I L G R T Y R V V P G E E E Q K F E V E K Y I V H K E F D D D T Y D N D I A L L Q L K S D S S R C A Q E S S V V R T V C L P P A D L Q L P D W T E C E L S G Y G K H E A L S P F Y S E R L K E A H V R L Y P S S R C T S Q H L L N R T V T D N M L C A G D T R S G G P Q A N L H D A C Q G D S G G P L V C L N D G R M T L V G I I S W G L G C G Q K D V P G V Y T K V T N Y L D W I R D N M R P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).
Additional Targets
PLAT
Alternative names
Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, PLAT
Recommended products
Recombinant human Tissue Plasminogen Activator protein, Two Chain (Active) is a Human Full Length protein, in the 36 to 562 aa range, expressed in CHO, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- CHO cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- 3,147,288 IU/mg (Relative to WHO International Standard for Human Recombinant Tissue Plasminogen Activator, NIBSC 98/714).
- Accession
- P00750-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 9.52% HEPES, 0.58% Sodium chloride
Sequence info
Amino acid sequence
- S Y Q V I C R D E K T Q M I Y Q Q H Q S W L R P V L R S N R V E Y C W C N S G R A Q C H S V P V K S C S E P R C F N G G T C Q Q A L Y F S D F V C Q C P E G F A G K C C E I D T R A T C Y E D Q G I S Y R G T W S T A E S G A E C T N W N S S A L A Q K P Y S G R R P D A I R L G L G N H N Y C R N P D R D S K P W C Y V F K A G K Y S S E F C S T P A C S E G N S D C Y F G N G S A Y R G T H S L T E S G A S C L P W N S M I L I G K V Y T A Q N P S A Q A L G L G K H N Y C R N P D G D A K P W C H V L K N R R L T W E Y C D V P S C S T C G L R Q Y S Q P Q F R I K G G L F A D I A S H P W Q A A I F A K H R R S P G E R F L C G G I L I S S C W I L S A A H C F Q E R F P P H H L T V I L G R T Y R V V P G E E E Q K F E V E K Y I V H K E F D D D T Y D N D I A L L Q L K S D S S R C A Q E S S V V R T V C L P P A D L Q L P D W T E C E L S G Y G K H E A L S P F Y S E R L K E A H V R L Y P S S R C T S Q H L L N R T V T D N M L C A G D T R S G G P Q A N L H D A C Q G D S G G P L V C L N D G R M T L V G I I S W G L G C G Q K D V P G V Y T K V T N Y L D W I R D N M R P
- Accession
- P00750
- Protein length
- Full Length
- Predicted molecular weight
- 59.04 kDa
- Amino acids
- 36 to 562
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Activated from single-chain form with immobilized plasmin. 100% complex formation with Human PAI1.
General info
- Function
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).
- Sequence similarities
Belongs to the peptidase S1 family.
- Post-translational modifications
The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Extinction Coefficient: 1.9
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.
- Biological function summary
TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.
- Pathways
TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.
- Associated diseases and disorders
TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.
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SDS-PAGE - Recombinant human Tissue Plasminogen Activator protein, Two Chain (Active) (ab92637)
10% SDS-PAGE
Lane 1: TPA Tissue Plasminogen Activator (3 ug) Non-reduced
Lane 2: TPA Tissue Plasminogen Activator (3 ug) Reduced
Lane 3: Prestained Standard
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