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Recombinant human Tissue Plasminogen Activator protein, Two Chain (Active) is a Human Full Length protein, in the 36 to 562 aa range, expressed in CHO, with >95% purity and suitable for SDS-PAGE, FuncS.

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Purity

>95% SDS-PAGE

Expression system

CHO cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Sequence

S Y Q V I C R D E K T Q M I Y Q Q H Q S W L R P V L R S N R V E Y C W C N S G R A Q C H S V P V K S C S E P R C F N G G T C Q Q A L Y F S D F V C Q C P E G F A G K C C E I D T R A T C Y E D Q G I S Y R G T W S T A E S G A E C T N W N S S A L A Q K P Y S G R R P D A I R L G L G N H N Y C R N P D R D S K P W C Y V F K A G K Y S S E F C S T P A C S E G N S D C Y F G N G S A Y R G T H S L T E S G A S C L P W N S M I L I G K V Y T A Q N P S A Q A L G L G K H N Y C R N P D G D A K P W C H V L K N R R L T W E Y C D V P S C S T C G L R Q Y S Q P Q F R I K G G L F A D I A S H P W Q A A I F A K H R R S P G E R F L C G G I L I S S C W I L S A A H C F Q E R F P P H H L T V I L G R T Y R V V P G E E E Q K F E V E K Y I V H K E F D D D T Y D N D I A L L Q L K S D S S R C A Q E S S V V R T V C L P P A D L Q L P D W T E C E L S G Y G K H E A L S P F Y S E R L K E A H V R L Y P S S R C T S Q H L L N R T V T D N M L C A G D T R S G G P Q A N L H D A C Q G D S G G P L V C L N D G R M T L V G I I S W G L G C G Q K D V P G V Y T K V T N Y L D W I R D N M R P

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Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant human Tissue Plasminogen Activator protein, Two Chain (Active) is a Human Full Length protein, in the 36 to 562 aa range, expressed in CHO, with >95% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>95% SDS-PAGE

Expression system

CHO cells

Applications

SDS-PAGE, FuncS

Biological activity

3,147,288 IU/mg (Relative to WHO International Standard for Human Recombinant Tissue Plasminogen Activator, NIBSC 98/714).

Predicted molecular weight

59.04 kDa

Amino acids

36 to 562

Sequence

S Y Q V I C R D E K T Q M I Y Q Q H Q S W L R P V L R S N R V E Y C W C N S G R A Q C H S V P V K S C S E P R C F N G G T C Q Q A L Y F S D F V C Q C P E G F A G K C C E I D T R A T C Y E D Q G I S Y R G T W S T A E S G A E C T N W N S S A L A Q K P Y S G R R P D A I R L G L G N H N Y C R N P D R D S K P W C Y V F K A G K Y S S E F C S T P A C S E G N S D C Y F G N G S A Y R G T H S L T E S G A S C L P W N S M I L I G K V Y T A Q N P S A Q A L G L G K H N Y C R N P D G D A K P W C H V L K N R R L T W E Y C D V P S C S T C G L R Q Y S Q P Q F R I K G G L F A D I A S H P W Q A A I F A K H R R S P G E R F L C G G I L I S S C W I L S A A H C F Q E R F P P H H L T V I L G R T Y R V V P G E E E Q K F E V E K Y I V H K E F D D D T Y D N D I A L L Q L K S D S S R C A Q E S S V V R T V C L P P A D L Q L P D W T E C E L S G Y G K H E A L S P F Y S E R L K E A H V R L Y P S S R C T S Q H L L N R T V T D N M L C A G D T R S G G P Q A N L H D A C Q G D S G G P L V C L N D G R M T L V G I I S W G L G C G Q K D V P G V Y T K V T N Y L D W I R D N M R P

Accession
P00750-1
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Concentration
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Storage buffer

pH: 7.4
Constituents: 9.52% HEPES, 0.58% Sodium chloride

Specifications

Form

Liquid

Additional notes

Activated from single-chain form with immobilized plasmin. 100% complex formation with Human PAI1.

General info

Function

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).

Sequence similarities

Belongs to the peptidase S1 family.

Post-translational modifications

The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Extinction Coefficient: 1.9

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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