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AB152750

Recombinant Human TLE4 protein

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Recombinant Human TLE4 protein is a Human Full Length protein, in the 1 to 773 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

GRG4, KIAA1261, TLE4, Transducin-like enhancer protein 4, Grg-4, Groucho-related protein 4

1 Images
SDS-PAGE - Recombinant Human TLE4 protein (AB152750)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TLE4 protein (AB152750)

12.5% SDS-PAGE analysis of ab152750 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q04727

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MIRDLSKMYPQTRHPAPHQPAQPFKFTISESCDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNAICAQVIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQLQAQHLSHGHGLPVPLTPHPSGLQPPAIPPIGSSAGLLALSSALGGQSHLPIKDEKKHHDNDHQRDRDSIKSSSVSPSASFRGAEKHRNSADYSSESKKQKTEEKEIAARYDSDGEKSDDNLVVDVSNEDPSSPRGSPAHSPRENGLDKTRLLKKDAPISPASIASSSSTPSSKSKELSLNEKSTTPVSKSNTPTPRTDAPTPGSNSTPGLRPVPGKPPGVDPLASSLRTPMAVPCPYPTPFGIVPHAGMNGELTSPGAAYAGLHNISPQMSAAAAAAAAAAAYGRSPVVGFDPHHHMRVPAIPPNLTGIPGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCRLLPDGRTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMENSNVEVLHVTKPDKYQLHLHESCVLSLKFAHCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY","proteinLength":"Full Length","predictedMolecularWeight":"110.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":773,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q04727","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transducin-like enhancer of split 4 (TLE4) is a transcriptional corepressor involved in the regulation of gene expression. It is also known as ESG (Enhancer of split groucho) or GRG4. TLE4 belongs to the Groucho/TLE protein family which shares a general structure characterized by WD40 repeats. The protein has a molecular mass of about 82 kDa. TLE4 expresses in several tissues including the brain heart and peripheral blood leukocytes indicating its broad functional importance.
Biological function summary

TLE4 participates in the repression of transcription by forming a multiprotein complex. It acts as a corepressor that interacts with various transcription factors often modulating the expression of genes involved in developmental processes. TLE4 commonly partners with transcription factors from the basic helix-loop-helix (bHLH) family which play a significant role in the regulation of neuronal and muscular development. The repression activity of TLE4 makes it a temporal regulator helping maintain and coordinate gene expression during cellular differentiation.

Pathways

TLE4 functions within the Wnt signaling and Notch signaling pathways. Both of these pathways are important for cell fate determination and organ development. In the Wnt pathway TLE4 interacts with beta-catenin to modulate transcriptional activity contributing to cellular responses to external signals. In the Notch signaling pathway TLE4 associates with core transcription factors like CSL (CBF1/Suppressor of Hairless/Lag1) to regulate the expression of Notch target genes facilitating diverse developmental processes.

TLE4 has links to hematological malignancies and neurological conditions. Its dysregulation can contribute to conditions like acute myeloid leukemia (AML) alongside proteins such as RUNX1 which play a role in hematopoiesis and myeloid lineage development. Additionally altered TLE4 expression has associations with neurodevelopmental disorders as its interaction with key proteins in neuronal differentiation is disturbed affecting neuronal function and development.
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Specifications

Form

Liquid

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General info

Function

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity). Involved in transcriptional repression of GNRHR and enhances MSX1-mediated transcriptional repression of CGA/alpha-GSU (By similarity).

Sequence similarities

Belongs to the WD repeat Groucho/TLE family.

Post-translational modifications

Phosphorylated. PAX5 binding increases phosphorylation.. Ubiquitinated by XIAP/BIRC4.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity). Involved in transcriptional repression of GNRHR and enhances MSX1-mediated transcriptional repression of CGA/alpha-GSU (By similarity).
See full target information TLE4
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Complementary Products

Primary Antibodies

AB64833

Anti-TLE4 antibody

Western blot - Anti-TLE4 antibody (AB64833)

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  • 2

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