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AB125563

Recombinant human TLK1 protein

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Recombinant human TLK1 protein is a Human Full Length protein, in the 1 to 766 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

KIAA0137, TLK1, Serine/threonine-protein kinase tousled-like 1, PKU-beta, Tousled-like kinase 1

4 Images
Functional Studies - Recombinant human TLK1 protein (AB125563)
  • FuncS

Unknown

Functional Studies - Recombinant human TLK1 protein (AB125563)

Sample Kinase Assay showing the specific activity of ab125563 to be 6.2 nmol/min/mg.

Functional Studies - Recombinant human TLK1 protein (AB125563)
  • FuncS

Unknown

Functional Studies - Recombinant human TLK1 protein (AB125563)

The specific activity of TLK1 (ab125563) was determined to be 7.1 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human TLK1 protein (AB125563)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TLK1 protein (AB125563)

SDS-PAGE analysis of ab125563.

SDS-PAGE - Recombinant human TLK1 protein (AB125563)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TLK1 protein (AB125563)

SDS PAGE analysis of ab125563

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE, WB

applications

Biologically active

Yes

Biological activity

The specific activity of ab125563 was determined to be 6.2 - 7.1 nmol/min/mg.

Accession

Q9UKI8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY","proteinLength":"Full Length","predictedMolecularWeight":"135 kDa","actualMolecularWeight":null,"aminoAcidEnd":766,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9UKI8","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Toutatis-like kinase 1 (TLK1) also known as serine/threonine-protein kinase tousled-like 1 is a kinase that plays a role in DNA repair and chromatin assembly. Its molecular mass is approximately 85 kDa. It is widely expressed in various human tissues with notable expression in the testis and brain. TLK1 primarily functions during DNA replication where it phosphorylates substrates that are vital for maintaining genomic stability.
Biological function summary

TLK1 contributes significantly to chromatin remodeling and DNA repair by influencing histone dynamics. TLK1 often acts in concert with other proteins as part of regulatory complexes modulating DNA accessibility during replication and repair processes. As a kinase it phosphorylates and activates targets like Asf1 a histone chaperone impacting nucleosome assembly.

Pathways

TLK1 plays a significant role in the DNA damage response and cell cycle regulation pathways. It is closely related to the ATM/ATR pathway which is central to the cellular response to DNA damage. TLK1 also interacts with proteins like Asf1 influencing replication-coupled chromatin assembly and cell cycle progression by modifying histones and other structural proteins.

TLK1 has been linked to certain cancers and neurodegenerative disorders. In cancer disruptions in TLK1 activity can lead to genomic instability contributing to tumorigenesis. TLK1 also connects with proteins like ASF1A in these contexts affecting cell proliferation and survival. In neurodegenerative diseases alterations in TLK1 function might impact neurological pathways related to DNA repair though specific mechanisms remain under investigation.
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Specifications

Form

Liquid

Additional notes

Purity determined to be >90% by densitometry.

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General info

Function

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.
See full target information TLK1
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Cell Lines & Lysates

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Advanced Validation

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Cell Lines & Lysates

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