Recombinant human TLK2 protein
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Recombinant human TLK2 protein is a Human Fragment protein, in the 388 to 772 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.
View Alternative Names
Serine/threonine-protein kinase tousled-like 2, HsHPK, PKU-alpha, Tousled-like kinase 2, TLK2
- FuncS
Unknown
Functional Studies - Recombinant human TLK2 protein (AB89855)
Kinase Assay demonstrating specific activity of ab89855
- FuncS
Unknown
Functional Studies - Recombinant human TLK2 protein (AB89855)
The specific activity of TLK2 (ab89855) was determined to be 19 nmol/min/mg as per activity assay protocol and was equivalent to 85 nmol/min/mg as per radiometric assay
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human TLK2 protein (AB89855)
SDS-PAGE showing ab89855 at approximately 67kDa
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human TLK2 protein (AB89855)
SDS PAGE analysis of ab89855
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TLK2 acts as a modulator of chromatin assembly by interacting with the ASF1 proteins forming a critical complex in cellular processes. Its activity is essential for the proper progression of the S-phase during the cell cycle. This function connects TLK2 to the maintenance of genomic stability largely through regulating histone incorporation. Such interactions are necessary for the preservation of the genomic integrity during DNA replication.
Pathways
TLK2 significantly impacts cell cycle regulation and DNA damage response pathways. Its participation in the cell cycle pathway intersecting with other kinases is evident as it influences the activity of proteins like CHK1 impacting the G2/M checkpoint. TLK2's involvement in the DNA damage response closely related to the ATM/ATR signaling pathway positions it as an essential player in responding to DNA insults and maintaining cell survival during stress.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation (PubMed : 10523312, PubMed : 11470414, PubMed : 12660173, PubMed : 12955071, PubMed : 29955062, PubMed : 33323470, PubMed : 9427565). Phosphorylates the chromatin assembly factors ASF1A and ASF1B (PubMed : 11470414, PubMed : 20016786, PubMed : 29955062, PubMed : 35136069). Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly (PubMed : 20016786). Negative regulator of amino acid starvation-induced autophagy (PubMed : 22354037).
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Post-translational modifications
Phosphorylated at Ser-750, probably by CHEK1.. Autophosphorylated; phosphorylation promotes the assembly of higher order oligomers and enzymatic activity.
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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