Recombinant Human TLR2 protein (His tag) is a Human Fragment protein, in the 19 to 588 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
K E E S S N Q A S L S C D R N G I C K G S S G S L N S I P S G L T E A V K S L D L S N N R I T Y I S N S D L Q R C V N L Q A L V L T S N G I N T I E E D S F S S L G S L E H L D L S Y N Y L S N L S S S W F K P L S S L T F L N L L G N P Y K T L G E T S L F S H L T K L Q I L R V G N M D T F T K I Q R K D F A G L T F L E E L E I D A S D L Q S Y E P K S L K S I Q N V S H L I L H M K Q H I L L L E I F V D V T S S V E C L E L R D T D L D T F H F S E L S T G E T N S L I K K F T F R N V K I T D E S L F Q V M K L L N Q I S G L L E L E F D D C T L N G V G N F R A S D N D R V I D P G K V E T L T I R R L H I P R F Y L F Y D L S T L Y S L T E R V K R I T V E N S K V F L V P C L L S Q H L K S L E Y L D L S E N L M V E E Y L K N S A C E D A W P S L Q T L I L R Q N H L A S L E K T G E T L L T L K N L T N I D I S K N S F H S M P E T C Q W P E K M K Y L N L S S T R I H S V T G C I P K T L E I L D V S N N N L N L F S L N L P Q L K E L Y I S R N K L M T L P D A S L L P M L L V L K I S R N A I T T F S K E Q L D S F H T L K T L E A G G N N F I C S C E F L S F T Q E Q Q A L A K V L I D W P A N Y L C D S P S H V R G Q Q V Q D V R L S V S E C H R T L E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:17889651, PubMed:21078852). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed:10426995, PubMed:10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed:11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed:16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed:16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed:15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (PubMed:16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (By similarity).
Alternative names
CD282, TIL4, TLR2, Toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4
Recommended products
Recombinant Human TLR2 protein (His tag) is a Human Fragment protein, in the 19 to 588 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O60603-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- K E E S S N Q A S L S C D R N G I C K G S S G S L N S I P S G L T E A V K S L D L S N N R I T Y I S N S D L Q R C V N L Q A L V L T S N G I N T I E E D S F S S L G S L E H L D L S Y N Y L S N L S S S W F K P L S S L T F L N L L G N P Y K T L G E T S L F S H L T K L Q I L R V G N M D T F T K I Q R K D F A G L T F L E E L E I D A S D L Q S Y E P K S L K S I Q N V S H L I L H M K Q H I L L L E I F V D V T S S V E C L E L R D T D L D T F H F S E L S T G E T N S L I K K F T F R N V K I T D E S L F Q V M K L L N Q I S G L L E L E F D D C T L N G V G N F R A S D N D R V I D P G K V E T L T I R R L H I P R F Y L F Y D L S T L Y S L T E R V K R I T V E N S K V F L V P C L L S Q H L K S L E Y L D L S E N L M V E E Y L K N S A C E D A W P S L Q T L I L R Q N H L A S L E K T G E T L L T L K N L T N I D I S K N S F H S M P E T C Q W P E K M K Y L N L S S T R I H S V T G C I P K T L E I L D V S N N N L N L F S L N L P Q L K E L Y I S R N K L M T L P D A S L L P M L L V L K I S R N A I T T F S K E Q L D S F H T L K T L E A G G N N F I C S C E F L S F T Q E Q Q A L A K V L I D W P A N Y L C D S P S H V R G Q Q V Q D V R L S V S E C H R T L E H H H H H H
- Accession
- O60603
- Protein length
- Fragment
- Predicted molecular weight
- 65.5 kDa
- Amino acids
- 19 to 588
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:17889651, PubMed:21078852). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed:10426995, PubMed:10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed:11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed:16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed:16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed:15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (PubMed:16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (By similarity).
- Sequence similarities
Belongs to the Toll-like receptor family.
- Post-translational modifications
Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Toll-like receptor 2 (TLR2) also called CD282 is a protein that detects molecules from bacteria and fungi. It has a mass of about 90 kDa. TLR2 is expressed on the surface of immune cells such as monocytes macrophages and neutrophils. This receptor recognizes pathogen-associated molecular patterns (PAMPs) and activates the immune response.
- Biological function summary
TLR2 initiates signaling pathways that lead to the production of cytokines and chemokines helping to control infection. It forms dimers with other TLRs like TLR1 or TLR6 to expand its recognition range of microbial components. TLR2's activity is important for balancing pathogen recognition and avoiding overactive immune response. On macrophages TLR2 activation enhances their phagocytic ability.
- Pathways
TLR2 plays roles in innate immune signaling pathways and inflammation pathways. It contributes significantly to the NF-kB and MAPK pathways which regulate inflammation and immune response. TLR2 often cooperates with proteins like MyD88 and TRAF6 in these pathways leading to downstream activation of transcription factors and production of pro-inflammatory molecules.
- Associated diseases and disorders
TLR2 is associated with inflammatory and infectious diseases including sepsis and tuberculosis. During tuberculosis interactions between TLR2 and its ligands trigger immune responses that are important for bacterial control. Additionally TLR2’s overactivation links to sepsis by causing excessive inflammation. Researchers study TLR2 for potential therapeutic targeting in these diseases assessing how modulation can improve outcomes or reduce detrimental inflammation.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human TLR2 protein (His tag) (ab222352)
15% SDS-PAGE analysis of 3 μg ab222352.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com