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AB314625

Recombinant Human TMEM59 Protein (His Tag)

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Recombinant Human TMEM59 Protein (His Tag) is a Human Full Length protein, in the 37 to 323 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

C1orf8, HSPC001, UNQ169/PRO195, TMEM59, Transmembrane protein 59, Liver membrane-bound protein

1 Images
SDS-PAGE - Recombinant Human TMEM59 Protein (His Tag) (AB314625)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TMEM59 Protein (His Tag) (AB314625)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9BXS4

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Storage buffer

pH: 7.4 - 8 Constituents: PBS, 6% Trehalose

storage-buffer

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Complementary Products

Primary Antibodies

AB97597

Anti-TMEM59 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM59 antibody (AB97597)

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Cell Lines & Lysates

AB266468

Human TMEM59 knockout HEK-293T cell line

Sanger Sequencing - Human TMEM59 knockout HEK-293T cell line (AB266468)

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Cell Lines & Lysates

AB263392

Human TMEM59 knockout HEK-293T cell lysate

Sanger Sequencing - Human TMEM59 knockout HEK-293T cell lysate (AB263392)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"AFDSVLGDTASCHRACQLTYPLHTYPKEEELYACQRGCRLFSICQFVDDGIDLNRTKLECESACTEAYSQSDEQYACHLGCQNQLPFAELRQEQLMSLMPKMHLLFPLTLVRSFWSDMMDSAQSFITSSWTFYLQADDGKIVIFQSKPEIQYAPHLEQEPTNLRESSLSKMSYLQMRNSQAHRNFLEDGESDGFLRCLSLNSGWILTTTLVLSVMVLLWICCATVATAVEQYVPSEKLSIYGDLEFMNEQKLNRYPASSLVVVRSKTEDHEEAGPLPTKVNLAHSEI","proteinLength":"Full Length","predictedMolecularWeight":"35.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":323,"aminoAcidStart":37,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BXS4","tags":[{"tag":"10x His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
The product can be stored for up to 12 months
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM59 also known as Transmembrane Protein 59 plays a role in protein glycosylation and modulation of the cell surface expression of certain proteins. It has a molecular mass of approximately 39 kDa. TMEM59 is expressed in a variety of tissues including the brain lung and kidney where it localizes to the endoplasmic reticulum and Golgi apparatus. The protein's structure suggests membrane spanning properties that contribute to its function in cell regulation.
Biological function summary

TMEM59 contributes to proper neuronal function by modulating the cell surface expression of proteins involved in neuron-neuron communications. This regulation has implications for membrane protein function and synaptic stability. TMEM59 participates in certain bionetworks interacting with protein complexes that influence cell signaling pathways. Such interactions play significant roles in controlling protein trafficking and localization within cells impacting the cellular response to environmental signals.

Pathways

TMEM59 influences processes like the Wnt signaling pathway affecting cell fate decisions and embryonic development. It interacts with proteins such as β-catenin and affects their stability and function. TMEM59 also participates in the autophagy-dependent pathways modulating cellular mechanisms involved in the degradation of damaged proteins and organelles therefore maintaining cellular homeostasis.

TMEM59 has links to neurodegenerative diseases including Alzheimer's disease and schizophrenia. TMEM59's regulation of proteins like APP (Amyloid precursor protein) is significant. Disruptions in its function may contribute to aberrant protein aggregation commonly seen in Alzheimer's disease pathology. Additionally disruptions in TMEM59 expression or function could affect cognitive processes and synaptic connections related to schizophrenia as it influences the trafficking of receptor proteins important for synaptic signaling.
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Specifications

Form

Lyophilized

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General info

Function

Acts as a regulator of autophagy in response to S.aureus infection by promoting activation of LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C). Acts by interacting with ATG16L1, leading to promote a functional complex between LC3 and ATG16L1 and promoting LC3 lipidation and subsequent activation of autophagy (PubMed : 23376921, PubMed : 27273576). Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP (PubMed : 20427278). Inhibits APP transport to the cell surface and further shedding (PubMed : 20427278).

Sequence similarities

Belongs to the TMEM59 family.

Post-translational modifications

N-glycosylated.

Subcellular localisation

Late endosome membrane

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Product protocols

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Target data

Acts as a regulator of autophagy in response to S.aureus infection by promoting activation of LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C). Acts by interacting with ATG16L1, leading to promote a functional complex between LC3 and ATG16L1 and promoting LC3 lipidation and subsequent activation of autophagy (PubMed : 23376921, PubMed : 27273576). Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP (PubMed : 20427278). Inhibits APP transport to the cell surface and further shedding (PubMed : 20427278).
See full target information TMEM59
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Product promise

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