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AB314626

Recombinant Human TMEM65 Protein (His Tag)

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Recombinant Human TMEM65 Protein (His Tag) is a Human Fragment protein, in the 63 to 240 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

Transmembrane protein 65, TMEM65

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SDS-PAGE - Recombinant Human TMEM65 Protein (His Tag) (AB314626)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TMEM65 Protein (His Tag) (AB314626)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q6PI78

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Storage buffer

pH: 7.4 - 8 Constituents: PBS, 6% Trehalose

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEAPPPTPGQLRYVFIHNAIPFIGFGFLDNAIMIVAGTHIEMSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRLSTHLGKAVGVTIGCILGMFPLIFFGGGEEDEKLETKS","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":"35.1 kDa","aminoAcidEnd":240,"aminoAcidStart":63,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6PI78","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
The product can be stored for up to 12 months
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMEM65 also known as Transmembrane protein 65 is a protein with a mass of approximately 27 kDa. It is a component of mitochondria largely expressed in cardiac and skeletal muscles. TMEM65 resides in the inner mitochondrial membrane where it plays a role in maintaining the structural integrity of the organelle. Its function is linked to the transmembrane transport of ions and metabolites contributing to mitochondrial efficiency.
Biological function summary

TMEM65 influences cellular energy dynamics. This protein interacts with components of the mitochondrial contact site and cristae organizing system (MICOS) complex which is important for maintaining cristae structure. The protein's role in energy production underlines its importance especially in tissues with high energy demand. By stabilizing mitochondrial architecture TMEM65 facilitates effective oxidative phosphorylation supporting ATP synthesis.

Pathways

TMEM65 significantly impacts the oxidative phosphorylation and electron transport chain pathways. It plays a role by interacting with proteins such as Nicotinamide Adenine Dinucleotide (NADH) dehydrogenase. This interaction helps in electron transport essential for generating the proton gradient used to drive ATP synthesis a fundamental cellular process to provide energy.

TMEM65 mutations associate with cardiomyopathy and neurodegenerative diseases. In cardiomyopathy faulty TMEM65 disrupts normal mitochondrial function affecting heart muscle and leading to impaired cardiac contractility. TMEM65's role here connects it to proteins like ATP synthase further influencing its involvement in energy metabolism disorders. Understanding these interactions could offer insights into potential therapeutic approaches targeting mitochondrial function.
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Specifications

Form

Lyophilized

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General info

Function

Essential for maintaining proper cardiac intercalated disk (ICD) structure and function as well as cardiac conduction velocity in the heart. Its association with SCN1B is required for stabilizing the perinexus in the ICD and for localization of GJA1 and SCN5A to the ICD. May regulate the function of the gap junction protein GJA1 and may contribute to the stability and proper localization of GJA1 to cardiac intercalated disk thereby regulating gap junction communication (By similarity). May also play a role in the regulation of mitochondrial respiration and mitochondrial DNA copy number maintenance (PubMed : 28295037).

Subcellular localisation

Mitochondrion inner membrane

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Product protocols

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Target data

Essential for maintaining proper cardiac intercalated disk (ICD) structure and function as well as cardiac conduction velocity in the heart. Its association with SCN1B is required for stabilizing the perinexus in the ICD and for localization of GJA1 and SCN5A to the ICD. May regulate the function of the gap junction protein GJA1 and may contribute to the stability and proper localization of GJA1 to cardiac intercalated disk thereby regulating gap junction communication (By similarity). May also play a role in the regulation of mitochondrial respiration and mitochondrial DNA copy number maintenance (PubMed : 28295037).
See full target information TMEM65
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Complementary Products

Primary Antibodies

AB236861

Anti-TMEM65 antibody

Western blot - Anti-TMEM65 antibody (AB236861)

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