Recombinant human TNF alpha protein is a Human Full Length protein, in the 77 to 233 aa range with >98% purity, < 1 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab9642 protein is 17.4 kDa.
- Save time and ensure accurate results - use our TNF alpha protein as a control
- Optimal protein bioactivity and stability
- Available in different sizes to fit your experimental needs
- Cited in over 25 publications
Images
Publications
Filter by
- The Journal of clinical investigation 134:2024Gasdermin C sensitizes tumor cells to PARP inhibitor therapy in cancer models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuanglian Wang,Chiung-Wen Chang,Juan Huang,Shan Zeng,Xin Zhang,Mien-Chie Hung,Junwei HouPubMed 37883181
- Nature communications 14:62452023Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Sunshine,Andreas S Puschnik,Joseph M Replogle,Matthew T Laurie,Jamin Liu,Beth Shoshana Zha,James K Nuñez,Janie R Byrum,Aidan H McMorrow,Matthew B Frieman,Juliane Winkler,Xiaojie Qiu,Oren S Rosenberg,Manuel D Leonetti,Chun Jimmie Ye,Jonathan S Weissman,Joseph L DeRisi,Marco Y HeinPubMed 37803001
- Nature biomedical engineering 7:943-9582023High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaeid Ansaryan,Yen-Cheng Liu,Xiaokang Li,Augoustina Maria Economou,Christiane Sigrid Eberhardt,Camilla Jandus,Hatice AltugPubMed 37012313
- Molecular pharmaceutics 20:1884-18972023Discovery of a Non-competitive TNFR1 Antagonist Affibody with Picomolar Monovalent Potency That Does Not Affect TNFR2 Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNagamani Vunnam,MaryJane Been,Evan Huber,Carolyn Paulson,Sophia Szymonski,Benjamin J Hackel,Jonathan N SachsPubMed 36897792
- The Analyst 147:5409-54182022Multiplexed microfluidic chip for cell co-culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCraig Watson,Chao Liu,Ali Ansari,Helen C Miranda,Rodrigo A Somoza,Samuel E SenyoPubMed 36300548
- Nature communications 13:44952022Engineering micro oxygen factories to slow tumour progression via hyperoxic microenvironments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeili Wang,Huizhen Zheng,Jun Jiang,Zhi Li,Dongpeng Jiang,Xiangru Shi,Hui Wang,Jie Jiang,Qianqian Xie,Meng Gao,Jianhong Chu,Xiaoming Cai,Tian Xia,Ruibin LiPubMed 35918337
- Science advances 8:eabn72982022Development of a physiological insulin resistance model in human stem cell-derived adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMax Friesen,Andrew S Khalil,M Inmaculada Barrasa,Jacob F Jeppesen,David J Mooney,Rudolf JaenischPubMed 35714187
- Cells 11:2022Rhoptry Protein 7 (ROP7) Interacts with NLRP3 and Promotes Inflammasome Hyperactivation in THP-1-Derived Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijun Zhu,Wanjun Qi,Guang Yang,Yurong Yang,Yuwen Wang,Lulu Zheng,Yongfeng Fu,Xunjia ChengPubMed 35626667
- Science signaling 14:eabj28072021Adipocyte-derived exosomes may promote breast cancer progression in type 2 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaser Jafari,Manohar Kolla,Tova Meshulam,Jordan S Shafran,Yuhan Qiu,Allison N Casey,Isabella R Pompa,Christina S Ennis,Carla S Mazzeo,Nabil Rabhi,Stephen R Farmer,Gerald V DenisPubMed 34813359
- Frontiers in pharmacology 12:7351942021Cinacalcet Targets the Neurokinin-1 Receptor and Inhibits PKCδ/ERK/P65 Signaling to Alleviate Dextran Sulfate Sodium-Induced Colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuehong Chen,Huan Liu,Qiuping Zhang,Yubin Luo,Liang Wu,Yutong Zhong,Zhigang Tang,Yaoyu Pu,Chenyang Lu,Geng Yin,Qibing XiePubMed 34880751
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, HPLC, sELISA
- Biologically active
- Yes
Amino acid sequence
V R S S S R T P S D K P V A H V V A N P Q A E G Q L Q W L N R R A N A L L A N G V E L R D N Q L V V P S E G L Y L I Y S Q V L F K G Q G C P S T H V L L T H T I S R I A V S Y Q T K V N L L S A I K S P C Q R E T P E G A E A K P W Y E P I Y L G G V F Q L E K G D R L S A E I N R P D Y L D F A E S G Q V Y F G I I A L
Consider this alternative
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
Recommended products
Recombinant human TNF alpha protein is a Human Full Length protein, in the 77 to 233 aa range with >98% purity, < 1 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab9642 protein is 17.4 kDa.
- Save time and ensure accurate results - use our TNF alpha protein as a control
- Optimal protein bioactivity and stability
- Available in different sizes to fit your experimental needs
- Cited in over 25 publications
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, HPLC, sELISA
- Biologically active
- Yes
- Biological activity
- The ED50, as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D, is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 x 107 units/mg.
- Accession
- P01375-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
Constituents: 0.12% Sodium chloride, 0.049% Sodium phosphate
Sequence info
Amino acid sequence
- V R S S S R T P S D K P V A H V V A N P Q A E G Q L Q W L N R R A N A L L A N G V E L R D N Q L V V P S E G L Y L I Y S Q V L F K G Q G C P S T H V L L T H T I S R I A V S Y Q T K V N L L S A I K S P C Q R E T P E G A E A K P W Y E P I Y L G G V F Q L E K G D R L S A E I N R P D Y L D F A E S G Q V Y F G I I A L
- Accession
- P01375
- Protein length
- Full Length
- Predicted molecular weight
- 17.4 kDa
- Amino acids
- 77 to 233
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
>98% by HPLC analyses.Sterile filtered.
General info
- Function
The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells.
This supplementary information is collated from multiple sources and compiled automatically.- Sequence similarities
Belongs to the tumor necrosis factor family.
- Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our bioactive recombinant human TNF alpha protein ab9642 as a positive control in SDS-PAGE and HPLC. The rTNF-alpha has a molecular weight of 17.4 kDa and corresponds to the C-terminal extracellular domain of the full length transmembrane protein.
Analyze your ELISA data using the rTNF alpha ab9642 protein to generate and plot the standard curve.
Check out our protein gel staining guide for SDS-PAGE here
Check out our ELISA protocol for more information here.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunoprecipitation - Recombinant human TNF alpha protein (ab9642)
MLKL (phospho S345) was immunoprecipitated from 1mg of L-929 (Mouse connective tissue fibroblast cells) whole cell lysate treated with 20 ng/ml TNF alpha (ab9642) + 100 nM Smac mimetic + 20 μM z-VAD compound (Z-VAD(OH)-FMK, Irreversible general caspase inhibitor ab120382) for 8h using Anti-MLKL (phospho S345) antibody [EPR9515(2)] ab196436 at 1/150 dilution. Western blot was performed from the immunoprecipitate using Anti-MLKL (phospho S345) antibody [EPR9515(2)] ab196436 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: L-929 whole cell lysate treated with 20 ng/ml TNF alpha (ab9642) + 100 nM Smac mimetic+ 20 μM z-VAD compound (Z-VAD(OH)-FMK, Irreversible general caspase inhibitor ab120382) for 8h;10 μg (Input).
Lane 2: Anti-MLKL (phospho S345) antibody [EPR9515(2)] ab196436 IP in L-929 whole cell lysate treated with 20 ng/ml TNF alpha (ab9642) + 100 nM Smac mimetic+ 20 μM z-VAD compound (Z-VAD(OH)-FMK, Irreversible general caspase inhibitor ab120382) for 8h.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MLKL (phospho S345) antibody [EPR9515(2)] ab196436 in L-929 whole cell lysate treated with 20 ng/ml TNF alpha (ab9642) + 100 nM Smac mimetic+ 20 μM z-VAD compound (Z-VAD(OH)-FMK, Irreversible general caspase inhibitor ab120382) for 8h.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-MLKL (phospho S345) antibody [EPR9515(2)] (Anti-MLKL (phospho S345) antibody [EPR9515(2)] ab196436)
All lanes: Immunoprecipitation - Recombinant human TNF alpha protein (ab9642)
Predicted band size: 54 kDa
Observed band size: 54 kDa
ChIP - Recombinant human TNF alpha protein (ab9642)
Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with and without 20 ng/ml TNF-α (ab9642) for 60 minutes according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade ab218533 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach).
The ChIP data are consistent with the literature (PMID: 16135789).
Sandwich ELISA - Recombinant human TNF alpha protein (ab9642)
Standard curve for TNF alpha (Analyte: ab9642); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [2C8] to TNF alpha (ab8348) at 5μg/ml and Detector Antibody Rabbit polyclonal to TNF alpha (Anti-TNF alpha antibody ab9635) at 0.5μg/ml.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com