Recombinant human TNF alpha protein (Active) (ab155699)

Recombinant human TNF alpha protein (Active) is a Human Full Length protein in the 77 to 233 aa range with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA and Functional studies.The predicted molecular weight of ab155699 protein is 17.4 kDa.

- Save time and ensure accurate results - use our recombinant human TNF alpha as a control
- Optimal protein bioactivity and stability

Be the first to review this product! Submit a review

Images

Functional Studies - Recombinant human TNF alpha protein (Active) (AB155699), expandable thumbnail

Publications

Key facts

Purity: >95% SDS-PAGE
Endotoxin level: < 0.1 EU/µg
Expression system: HEK 293 cells
Tags: Tag free
Applications: SDS-PAGE, ELISA, FuncS
Biologically active: Yes

Amino acid sequence

V R S S S R T P S D K P V A H V V A N P Q A E G Q L Q W L N R R A N A L L A N G V E L R D N Q L V V P S E G L Y L I Y S Q V L F K G Q G C P S T H V L L T H T I S R I A V S Y Q T K V N L L S A I K S P C Q R E T P E G A E A K P W Y E P I Y L G G V F Q L E K G D R L S A E I N R P D Y L D F A E S G Q V Y F G I I A L

Consider this alternative

Reactivity data

Application	Reactivity	Dilution info	Notes
Application SDS-PAGE	Reactivity Reacts	Dilution info -	Notes -
Application ELISA	Reactivity Reacts	Dilution info -	Notes -
Application FuncS	Reactivity Reacts	Dilution info -	Notes -

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

See full target information TNF

Function

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

Key facts

Purity: >95% SDS-PAGE
Endotoxin level: < 0.1 EU/µg
Expression system: HEK 293 cells
Tags: Tag free
Applications: SDS-PAGE, ELISA, FuncS
Biologically active: Yes
Biological activity: Bioactivity is measured by its binding ability in a functional ELISA.
Immobilized ab155699 at 2 μg/mL ( 100 μl/well ) can bind Humira with a linear range of 0.2-2 ng/mL.
Accession: P01375-1
Animal free: No
Species: Human
Reconstitution: Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
Concentration
Storage buffer: pH: 7.4
Constituents: PBS, 5% Trehalose

Sequence info

Amino acid sequence

V R S S S R T P S D K P V A H V V A N P Q A E G Q L Q W L N R R A N A L L A N G V E L R D N Q L V V P S E G L Y L I Y S Q V L F K G Q G C P S T H V L L T H T I S R I A V S Y Q T K V N L L S A I K S P C Q R E T P E G A E A K P W Y E P I Y L G G V F Q L E K G D R L S A E I N R P D Y L D F A E S G Q V Y F G I I A L
Accession: P01375
Protein length: Full Length
Predicted molecular weight: 17.4 kDa
Amino acids: 77 to 233
Nature: Recombinant

Specifications

Form: Lyophilized
Additional notes: >95% SEC-MALS.Purified by ion exchange chromatography.

General info

Function: The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells.

This supplementary information is collated from multiple sources and compiled automatically.
Sequence similarities: Belongs to the tumor necrosis factor family.
Post-translational modifications: The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: Up to 12 months
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Ensure the validity of your result using our bioactive recombinant human TNF alpha ab155699 as a control in SDS-PAGE.

Analyze your human TNF alpha ELISA data using the ab155699 protein to generate and plot a standard curve.

Check out our protein gel staining guide for SDS-PAGE here

Check out our ELISA protocol for more information here.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

Functional Studies - Recombinant human TNF alpha protein (Active) (ab155699)
ab155699 induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D.
The EC₅₀ for this effect is 0.007-0.014 ng/ml (Routinely tested).
Functional Studies - Recombinant human TNF alpha protein (Active) (ab155699)
Neutralization assay shows that the cytotoxicity effect of ab155699 was inhibited by increasing concentration of Adalimumab.
The concentration of ab155699 used is 1 ng/mL.
The IC50 is 29 ng/mL (Routinely tested).
ELISA - Recombinant human TNF alpha protein (Active) (ab155699)
Immobilized ab155699 at 2 μg/mL (100 μL/well) binds Humira.
Linear range: 0.2-2 ng/mL (QC tested).
ELISA - Recombinant human TNF alpha protein (Active) (ab155699)
Immobilized ab155699 at 2 μg/mL (100 μL/well) binds Recombinant human TNF Receptor I protein (Fc Chimera Active) ab174046.
Linear range: 0.2-3 ng/mL (Routinely tested).
Functional Studies - Recombinant human TNF alpha protein (Active) (ab155699)
Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bindab155699 with an affinity constant of 0.255 nM, as determined in a SPR assay (Biacore T200) (Routinely tested).
SDS-PAGE - Recombinant human TNF alpha protein (Active) (ab155699)
Reduced ab155699 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >95%
The protein migrates as 17 kDa and 18 kDa under reducing conditions.
SEC-MALS - Recombinant human TNF alpha protein (Active) (ab155699)
Purity of ab155699 was more than 95% in HP-SEC, and around 47-60 kDa verified by SEC-MALS.