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Recombinant human TNF alpha protein (Active) is a Human Full Length protein, in the 77 to 233 aa range, expressed in HEK 293, with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

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Purity

>95% SDS-PAGE

Endotoxin level

< 0.1 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, ELISA, FuncS

Biologically active

Yes

Sequence

V R S S S R T P S D K P V A H V V A N P Q A E G Q L Q W L N R R A N A L L A N G V E L R D N Q L V V P S E G L Y L I Y S Q V L F K G Q G C P S T H V L L T H T I S R I A V S Y Q T K V N L L S A I K S P C Q R E T P E G A E A K P W Y E P I Y L G G V F Q L E K G D R L S A E I N R P D Y L D F A E S G Q V Y F G I I A L

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Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant human TNF alpha protein (Active) is a Human Full Length protein, in the 77 to 233 aa range, expressed in HEK 293, with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA, FuncS.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, ELISA, FuncS

Biological activity

Bioactivity is measured by its binding ability in a functional ELISA.

Immobilized ab155699 at 2 μg/mL ( 100 μl/well ) can bind Humira with a linear range of 0.2-2 ng/mL.

Predicted molecular weight

17.4 kDa

Amino acids

77 to 233

Sequence

V R S S S R T P S D K P V A H V V A N P Q A E G Q L Q W L N R R A N A L L A N G V E L R D N Q L V V P S E G L Y L I Y S Q V L F K G Q G C P S T H V L L T H T I S R I A V S Y Q T K V N L L S A I K S P C Q R E T P E G A E A K P W Y E P I Y L G G V F Q L E K G D R L S A E I N R P D Y L D F A E S G Q V Y F G I I A L

Accession
P01375-1
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Reconstitution

Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

Concentration
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Storage buffer

pH: 7.4
Constituents: PBS, 5% Trehalose

Specifications

Form

Lyophilized

Additional notes

>95% SEC-MALS.Purified by ion exchange chromatography.

General info

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918, PubMed:16829952, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819).

Sequence similarities

Belongs to the tumor necrosis factor family.

Post-translational modifications

The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Endotoxin levels determined by the LAL method.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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