Recombinant Human TNF Receptor II Protein (Active) is a Human Fragment protein, in the 24 to 206 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
P A Q V A F T P Y A P E P G S T C R L R E Y Y D Q T A Q M C C S K C S P G Q H A K V F C T K T S D T V C D S C E D S T Y T Q L W N W V P E C L S C G S R C S S D Q V E T Q A C T R E Q N R I C T C R P G W Y C A L S K Q E G C R L C A P L R K C R P G F G V A R P G T E T S D V V C K P C A P G T F S N T T S S T D I C R P H Q I C N V V A I P G N A S M D A V C T S T S P T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Alternative names
CD120b, TNFBR, TNFR2, TNFRSF1B, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II
Recommended products
Recombinant Human TNF Receptor II Protein (Active) is a Human Fragment protein, in the 24 to 206 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent inhibition of TNF alpha induced NF-ƙb activation in mouse RAW 264.7 NF-ƙb luciferase reporter cell line.
ED50 is ≤ 1.9 ug/ml, corresponding to a specific activity of 5.26 x 102 units/mg.
- Accession
- P20333-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- P A Q V A F T P Y A P E P G S T C R L R E Y Y D Q T A Q M C C S K C S P G Q H A K V F C T K T S D T V C D S C E D S T Y T Q L W N W V P E C L S C G S R C S S D Q V E T Q A C T R E Q N R I C T C R P G W Y C A L S K Q E G C R L C A P L R K C R P G F G V A R P G T E T S D V V C K P C A P G T F S N T T S S T D I C R P H Q I C N V V A I P G N A S M D A V C T S T S P T
- Accession
- P20333
- Protein length
- Fragment
- Predicted molecular weight
- 19.9 kDa
- Actual molecular weight
- 19.9 kDa
- Amino acids
- 24 to 206
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
- Post-translational modifications
Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Mass Spectrometry - Recombinant Human TNF Receptor II Protein (Active) (ab290100)
Mass determination by ESI-TOF. Predicted MW is 19899.44 Da (+/-10 Da by ESI-TOF). Observed MW is 19899.96 Da.
Biological Activity - Recombinant Human TNF Receptor II Protein (Active) (ab290100)
Fully biologically active determined by the dose dependent inhibition of TNF alpha induced NF-ƙb activation in mouse RAW 264.7 NF-ƙb luciferase reporter cell line.
ED50 is ≤ 1.9 ug/ml, corresponding to a specific activity of 5.26 x 102 units/mg.SDS-PAGE - Recombinant Human TNF Receptor II Protein (Active) (ab290100)
SDS-PAGE analysis of ab290100
HPLC - Recombinant Human TNF Receptor II Protein (Active) (ab290100)
HPLC analysis of ab290100
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Product protocols
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