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AB290100

Recombinant Human TNF Receptor II Protein (Active)

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Recombinant Human TNF Receptor II Protein (Active) is a Human Fragment protein, in the 24 to 206 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, Mass Spec, SDS-PAGE, FuncS.

View Alternative Names

CD120b, TNFBR, TNFR2, TNFRSF1B, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, TNFR-II

4 Images
Biological Activity - Recombinant Human TNF Receptor II Protein (Active) (AB290100)
  • Biological Activity

Supplier Data

Biological Activity - Recombinant Human TNF Receptor II Protein (Active) (AB290100)

Fully biologically active determined by the dose dependent inhibition of TNF alpha induced NF-ƙb activation in mouse RAW 264.7 NF-ƙb luciferase reporter cell line. ED50 is ≤ 1.9 ug/ml, corresponding to a specific activity of 5.26 x 102 units/mg.

Mass Spectrometry - Recombinant Human TNF Receptor II Protein (Active) (AB290100)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human TNF Receptor II Protein (Active) (AB290100)

Mass determination by ESI-TOF. Predicted MW is 19899.44 Da (+/-10 Da by ESI-TOF). Observed MW is 19899.96 Da.

HPLC - Recombinant Human TNF Receptor II Protein (Active) (AB290100)
  • HPLC

Supplier Data

HPLC - Recombinant Human TNF Receptor II Protein (Active) (AB290100)

HPLC analysis of ab290100

SDS-PAGE - Recombinant Human TNF Receptor II Protein (Active) (AB290100)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TNF Receptor II Protein (Active) (AB290100)

SDS-PAGE analysis of ab290100

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS, Mass Spec, HPLC

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent inhibition of TNF alpha induced NF-ƙb activation in mouse RAW 264.7 NF-ƙb luciferase reporter cell line.

ED50 is ≤ 1.9 ug/ml, corresponding to a specific activity of 5.26 x 102 units/mg.

Accession

P20333

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }
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Sequence info

[{"sequence":"PAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPT","proteinLength":"Fragment","predictedMolecularWeight":"19.9 kDa","actualMolecularWeight":"19.9 kDa","aminoAcidEnd":206,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P20333","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

SDS-PAGE >= 95%

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General info

Function

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Post-translational modifications

Phosphorylated; mainly on serine residues and with a very low level on threonine residues.. A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.

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Product protocols

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Target data

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
See full target information TNFRSF1B
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Product promise

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