Recombinant Human TNFAIP3 Protein is a Human Fragment protein, in the 2 to 439 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
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Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
HPLC, MS, SDS-PAGE
- Biologically active
No
Amino acid sequence
A E Q V L P Q A L Y L S N M R K A V K I R E R T P E D I F K P T N G I I H H F K T M H R Y T L E M F R T C Q F C P Q F R E I I H K A L I D R N I Q A T L E S Q K K L N W C R E V R K L V A L K T N G D G N C L M H A T S Q Y M W G V Q D T D L V L R K A L F S T L K E T D T R N F K F R W Q L E S L K S Q E F V E T G L C Y D T R N W N D E W D N L I K M A S T D T P M A R S G L Q Y N S L E E I H I F V L C N I L R R P I I V I S D K M L R S L E S G S N F A P L K V G G I Y L P L H W P A Q E C Y R Y P I V L G Y D S H H F V P L V T L K D S G P E I R A V P L V N R D R G R F E D L K V H F L T D P E N E M K E K L L K E Y L M V I E I P V Q G W D H G T T H L I N A A K L D E A N L P K E I N L V D D Y F E L V Q H E Y K K W Q E N S E Q G R R E G H A Q N P M E P S V P Q L S L M D V K C E T P N C P F F M S V N T Q P L C H E C S E R R Q K N Q N K L P K L N S K P G P E G L P G M A L G A S R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of pro-inflammatory cytokines and IFN beta in LPS-tolerized macrophages.
Alternative names
OTUD7C, TNFAIP3, OTUD7C, Tumor necrosis factor alpha-induced protein 3, TNF alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20
Recommended products
Recombinant Human TNFAIP3 Protein is a Human Fragment protein, in the 2 to 439 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
Key facts
- Purity
>=95% HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
HPLC, MS, SDS-PAGE
- Biologically active
No
- Accession
- P21580-1
- Animal free
Yes
- Species
Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
A E Q V L P Q A L Y L S N M R K A V K I R E R T P E D I F K P T N G I I H H F K T M H R Y T L E M F R T C Q F C P Q F R E I I H K A L I D R N I Q A T L E S Q K K L N W C R E V R K L V A L K T N G D G N C L M H A T S Q Y M W G V Q D T D L V L R K A L F S T L K E T D T R N F K F R W Q L E S L K S Q E F V E T G L C Y D T R N W N D E W D N L I K M A S T D T P M A R S G L Q Y N S L E E I H I F V L C N I L R R P I I V I S D K M L R S L E S G S N F A P L K V G G I Y L P L H W P A Q E C Y R Y P I V L G Y D S H H F V P L V T L K D S G P E I R A V P L V N R D R G R F E D L K V H F L T D P E N E M K E K L L K E Y L M V I E I P V Q G W D H G T T H L I N A A K L D E A N L P K E I N L V D D Y F E L V Q H E Y K K W Q E N S E Q G R R E G H A Q N P M E P S V P Q L S L M D V K C E T P N C P F F M S V N T Q P L C H E C S E R R Q K N Q N K L P K L N S K P G P E G L P G M A L G A S R
- Accession
- Protein length
Fragment
- Predicted molecular weight
50.95 kDa
- Actual molecular weight
50.8 kDa
- Amino acids
2 to 439
- Nature
Recombinant
Specifications
- Form
Liquid
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of pro-inflammatory cytokines and IFN beta in LPS-tolerized macrophages.
- Sequence similarities
Belongs to the peptidase C64 family.
- Post-translational modifications
Proteolytically cleaved by MALT1 upon TCR stimulation; disrupts NF-kappa-B inhibitory function and results in increased IL-2 production. It is proposed that only a fraction of TNFAIP3 colocalized with TCR and CBM complex is cleaved, leaving the main TNFAIP3 pool intact.
- Subcellular localisation
Nucleus, Lysosome
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
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3 product images
Mass Spectrometry - Recombinant Human TNFAIP3 Protein (ab307487)
Mass determination by ESI-TOF. Predicted MW is 50952.56 Da (+/-10 Da by ESI-TOF). Observed MW is 50797.33 Da.
SDS-PAGE - Recombinant Human TNFAIP3 Protein (ab307487)
SDS-PAGE analysis of ab307487
HPLC - Recombinant Human TNFAIP3 Protein (ab307487)
HPLC analysis of ab307487
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