Recombinant Human TNFRSF1A Protein (Active) is a Human Fragment protein, in the 30 to 211 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, Biological Activity, HPLC.
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Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, Biological Activity, HPLC
- Biologically active
- Yes
Amino acid sequence
L V P H L G D R E K R D S V C P Q G K Y I H P Q N N S I C C T K C H K G T Y L Y N D C P G P G Q D T D C R E C E S G S F T A S E N H L R H C L S C S K C R K E M G Q V E I S S C T V D R D T V C G C R K N Q Y R H Y W S E N L F Q C F N C S L C L N G T V H L S C Q E K Q N T V C T C H A G F F L R E N E C V S C S N C K K S L E C T K L C L P Q I E N V K G T E D S G T T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application Biological Activity | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Alternative names
CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I
Recommended products
Recombinant Human TNFRSF1A Protein (Active) is a Human Fragment protein, in the 30 to 211 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, Biological Activity, HPLC.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, MS, Biological Activity, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent inhibition of TNF alpha-induced NF-kb activation in mouse RAW264.7 NF-kb luciferase reporter cell line. ED50 is ≤530 ng/ml, corresponding to a specific activity of 1.88 x 103 units/mg.
- Accession
- P19438-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- L V P H L G D R E K R D S V C P Q G K Y I H P Q N N S I C C T K C H K G T Y L Y N D C P G P G Q D T D C R E C E S G S F T A S E N H L R H C L S C S K C R K E M G Q V E I S S C T V D R D T V C G C R K N Q Y R H Y W S E N L F Q C F N C S L C L N G T V H L S C Q E K Q N T V C T C H A G F F L R E N E C V S C S N C K K S L E C T K L C L P Q I E N V K G T E D S G T T
- Accession
- P19438
- Protein length
- Fragment
- Predicted molecular weight
- 20.52 kDa
- Actual molecular weight
- 20.52 kDa
- Amino acids
- 30 to 211
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity by HPLC =95%
General info
- Function
Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
- Post-translational modifications
The soluble form is produced from the membrane form by proteolytic processing.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
SDS-PAGE - Recombinant Human TNFRSF1A Protein (Active) (ab283448)
SDS-PAGE analysis of ab283448.
Mass Spectrometry - Recombinant Human TNFRSF1A Protein (Active) (ab283448)
ESI-TOF analysis of ab283448.
Predicted MW is 20515.11 Da (+/- 10 Da by ESI-TOF). Observed MW is 20517.36 Da.
HPLC - Recombinant Human TNFRSF1A Protein (Active) (ab283448)
HPLC analysis of ab283448.
Biochemical assay - Recombinant Human TNFRSF1A Protein (Active) (ab283448)
Fully biologically active determined by the dose dependent inhibition of TNF alpha-induced NF-kb activation in mouse RAW264.7 NF-kb luciferase reporter cell line.
ED50 is ≤ 530 ng/ml, corresponding to a specific activity of 1.88 x 103 units/mg.
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Product protocols
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