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AB283448

Recombinant Human TNFRSF1A Protein (Active)

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Recombinant Human TNFRSF1A Protein (Active) is a Human Fragment protein, in the 30 to 211 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, Biological Activity, HPLC, FuncS.

View Alternative Names

CD120a, TNFAR, TNFR1, TNFRSF1A, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I

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Mass Spectrometry - Recombinant Human TNFRSF1A Protein (Active) (AB283448)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human TNFRSF1A Protein (Active) (AB283448)

ESI-TOF analysis of ab283448.

Predicted MW is 20515.11 Da (+/- 10 Da by ESI-TOF). Observed MW is 20517.36 Da.

Biochemical assay - Recombinant Human TNFRSF1A Protein (Active) (AB283448)
  • Biochemical assay

Supplier Data

Biochemical assay - Recombinant Human TNFRSF1A Protein (Active) (AB283448)

Fully biologically active determined by the dose dependent inhibition of TNF alpha-induced NF-kb activation in mouse RAW264.7 NF-kb luciferase reporter cell line. ED50 is ≤ 530 ng/ml, corresponding to a specific activity of 1.88 x 103 units/mg.

SDS-PAGE - Recombinant Human TNFRSF1A Protein (Active) (AB283448)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TNFRSF1A Protein (Active) (AB283448)

SDS-PAGE analysis of ab283448.

HPLC - Recombinant Human TNFRSF1A Protein (Active) (AB283448)
  • HPLC

Supplier Data

HPLC - Recombinant Human TNFRSF1A Protein (Active) (AB283448)

HPLC analysis of ab283448.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, SDS-PAGE, Biological Activity, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent inhibition of TNF alpha-induced NF-kb activation in mouse RAW264.7 NF-kb luciferase reporter cell line. ED50 is ≤530 ng/ml, corresponding to a specific activity of 1.88 x 103 units/mg.

Accession

P19438

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Biological Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"LVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTT","proteinLength":"Fragment","predictedMolecularWeight":"20.52 kDa","actualMolecularWeight":"20.52 kDa","aminoAcidEnd":211,"aminoAcidStart":30,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P19438","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

Purity by HPLC =95%

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General info

Function

Receptor for TNFSF2/TNF and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

Post-translational modifications

The soluble form is produced from the membrane form by proteolytic processing.. (Microbial infection) Glycosylated at Arg-376 by enteropathogenic E.coli protein NleB1 and S.typhimurium protein Ssek3: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions.

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Product protocols

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Target data

Receptor for TNFSF2/TNF and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
See full target information TNFRSF1A
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Product promise

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