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Recombinant Human TNFSF12 Protein (Active) is a Human Fragment protein, in the 93 to 249 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human TNFSF12 Protein (Active) (AB283416), expandable thumbnail
  • Functional Studies - Recombinant Human TNFSF12 Protein (Active) (AB283416), expandable thumbnail

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE

Biologically active

Yes

Amino acid sequence

R S A P K G R K T R A R R A I A A H Y E V H P R P G Q D G A Q A G V D G T V S G W E E A R I N S S S P L R Y N R Q I G E F I V T R A G L Y Y L Y C Q V H F D E G K A V Y L K L D L L V D G V L A L R C L E E F S A T A A S S L G P Q L R L C Q V S G L L A L R P G S S L R I R T L P W A H L K A A P F L T Y F G L F Q V H

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.

Alternative names

Recommended products

Recombinant Human TNFSF12 Protein (Active) is a Human Fragment protein, in the 93 to 249 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE

Biological activity

Fully biologically active determined by its ability to dose dependently proliferate human umbilical vein endothelial cells (HUVECs). ED50 is ≤16.7 ng/ml, corresponding to a specific activity of 6.1x105 units/mg.

Accession
O43508-1
Animal free

Yes

Species

Human

Reconstitution

Reconstitute in PBS

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

R S A P K G R K T R A R R A I A A H Y E V H P R P G Q D G A Q A G V D G T V S G W E E A R I N S S S P L R Y N R Q I G E F I V T R A G L Y Y L Y C Q V H F D E G K A V Y L K L D L L V D G V L A L R C L E E F S A T A A S S L G P Q L R L C Q V S G L L A L R P G S S L R I R T L P W A H L K A A P F L T Y F G L F Q V H

Accession

O43508

Protein length

Fragment

Predicted molecular weight

17.41 kDa

Amino acids

93 to 249

Nature

Recombinant

Specifications

Form

Lyophilized

General info

Function

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.

Sequence similarities

Belongs to the tumor necrosis factor family.

Post-translational modifications

The soluble form derives from the membrane form by proteolytic processing.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The TNF-like weak inducer of apoptosis (TWEAK) also known as CD255 is a cytokine involved in a variety of cellular processes. This protein has an approximate mass of 24 kDa. TWEAK is expressed in many tissues including the heart brain and spleen. It functions by binding to the fibroblast growth factor-inducible 14 (Fn14) receptor leading to the activation of downstream signaling pathways. TWEAK's role as a ligand places it in the tumor necrosis factor (TNF) superfamily highlighting its involvement in cell death and inflammation signaling.

Biological function summary

TWEAK modulates processes like cell proliferation differentiation and apoptosis. This cytokine affects the immune response and tissue regeneration becoming significant in processes that require tissue remodeling. While acting independently TWEAK does not form part of a larger protein complex. Instead it signals mainly through its exclusive receptor Fn14 on responsive cells triggering cellular responses that influence its biological activities.

Pathways

TWEAK plays an important role in the NF-kB and MAPK signaling pathways. These pathways are important for immune response regulation and cell survival. In these pathways TWEAK closely interacts with TNF receptor-associated factors (TRAFs) which facilitate TWEAK signaling. The connections to pathways involving inflammatory response highlight its significance in maintaining homeostasis within the cellular environment.

Associated diseases and disorders

TWEAK's activity links to conditions like cancer and rheumatoid arthritis. In cancer excessive TWEAK signaling can contribute to tumor growth and metastasis through its pro-inflammatory and pro-angiogenic effects. In rheumatoid arthritis TWEAK expression relates to joint inflammation and tissue damage where it interacts with proteins like IL-6 and TNF-alpha. These interactions suggest potential therapeutic targets by modulating TWEAK or its signaling partners to treat these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Recombinant Human TNFSF12 Protein (Active) (ab283416), expandable thumbnail

    SDS-PAGE - Recombinant Human TNFSF12 Protein (Active) (ab283416)

    SDS-PAGE analysis of ab283416.

  • Functional Studies - Recombinant Human TNFSF12 Protein (Active) (ab283416), expandable thumbnail

    Functional Studies - Recombinant Human TNFSF12 Protein (Active) (ab283416)

    Fully biologically active determined by its ability to dose dependently proliferate human umbilical vein endothelial cells (HUVECs). ED50 is ≤16.7 ng/ml, corresponding to a specific activity of 6.1x105 units/mg.

    Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

    Lot GR3398997-2

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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