Recombinant Human TNIP1 protein is a Human Fragment protein, in the 94 to 530 aa range, expressed in Escherichia coli, with >75% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S N V T A S P T A P A C P S D K P A P V Q K P P S S G T S S E F E V V T P E E Q N S P E S S S H A N A M A L G P L P R E D G N L M L H L Q R L E T T L S V C A E E P D H G Q L F T H L G R M A L E F N R L A S K V H K N E Q R T S I L Q T L C E Q L R K E N E A L K A K L D K G L E Q R D Q A A E R L R E E N L E L K K L L M S N G N K E G A S G R P G S P K M E G T G K K A V A G Q Q Q A S V T A G K V P E V V A L G A A E K K V K M L E Q Q R S E L L E V N K Q W D Q H F R S M K Q Q Y E Q K I T E L R Q K L A D L Q K Q V T D L E A E R E Q K Q R D F D R K L L L A K S K I E M E E T D K E Q L T A E A K E L R Q K V K Y L Q D Q L S P L T R Q R E Y Q E K E I Q R L N K A L E E A L S I Q T P P S S P P T A F G S P E G A G A L L R K Q E L V T Q N E L L K Q Q V K I F E E D F Q R E R S D R E R M N E E K E E L K K Q V E K L Q A Q V T L S N A Q L K A F K D E E K A R E A L R Q Q K R K A K A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating TAX1BP1 and A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG (PubMed:21885437). Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection.
Alternative names
KIAA0113, NAF1, TNIP1, TNFAIP3-interacting protein 1, A20-binding inhibitor of NF-kappa-B activation 1, HIV-1 Nef-interacting protein, Nef-associated factor 1, Nip40-1, Virion-associated nuclear shuttling protein, ABIN-1, Naf1, VAN, hVAN
Recommended products
Recombinant Human TNIP1 protein is a Human Fragment protein, in the 94 to 530 aa range, expressed in Escherichia coli, with >75% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q15025-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S N V T A S P T A P A C P S D K P A P V Q K P P S S G T S S E F E V V T P E E Q N S P E S S S H A N A M A L G P L P R E D G N L M L H L Q R L E T T L S V C A E E P D H G Q L F T H L G R M A L E F N R L A S K V H K N E Q R T S I L Q T L C E Q L R K E N E A L K A K L D K G L E Q R D Q A A E R L R E E N L E L K K L L M S N G N K E G A S G R P G S P K M E G T G K K A V A G Q Q Q A S V T A G K V P E V V A L G A A E K K V K M L E Q Q R S E L L E V N K Q W D Q H F R S M K Q Q Y E Q K I T E L R Q K L A D L Q K Q V T D L E A E R E Q K Q R D F D R K L L L A K S K I E M E E T D K E Q L T A E A K E L R Q K V K Y L Q D Q L S P L T R Q R E Y Q E K E I Q R L N K A L E E A L S I Q T P P S S P P T A F G S P E G A G A L L R K Q E L V T Q N E L L K Q Q V K I F E E D F Q R E R S D R E R M N E E K E E L K K Q V E K L Q A Q V T L S N A Q L K A F K D E E K A R E A L R Q Q K R K A K A
- Accession
- Q15025
- Protein length
- Fragment
- Predicted molecular weight
- 51.8 kDa
- Amino acids
- 94 to 530
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab101108 was purified using conventional chromatography techniques.
General info
- Function
Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating TAX1BP1 and A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG (PubMed:21885437). Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection.
- Post-translational modifications
Phosphorylation at Tyr-552 by SRC-family kinases recruits phosphoinositide-3-kinase (PI3K) PIK3CD:p85 heterodimer which results in integrin activation and leukocyte adhesion to activated endothelium during inflammation.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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SDS-PAGE - Recombinant Human TNIP1 protein (ab101108)
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