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AB172165

Recombinant Human Tollip protein

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Recombinant Human Tollip protein is a Human Full Length protein, in the 2 to 274 aa range, expressed in Escherichia coli, with >95%, 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Toll-interacting protein, TOLLIP

1 Images
SDS-PAGE - Recombinant Human Tollip protein (AB172165)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Tollip protein (AB172165)

SDS-PAGE gel of ab172165 under reduced (lane 1) and non-reduced (lane 2) conditions.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

1 EU/µg

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

Constituents: PBS, 0.88% Sodium chloride, 0.15% EDTA

storage-buffer

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Complementary Products

Primary Antibodies

AB187198

Anti-Tollip antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (AB187198)

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Primary Antibodies

AB193360

Anti-RAB7B antibody [EPR15727]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB7B antibody [EPR15727] (AB193360)

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Proteins & Peptides

AB158120

Recombinant Human CHRNB4 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human CHRNB4 protein (GST tag N-Terminus) (AB158120)

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  • 0
Assay Kits

AB241036

Cardiolipin Assay Kit

Functional Studies - Cardiolipin Assay Kit (AB241036)

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  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEPVEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"31 kDa","actualMolecularWeight":null,"aminoAcidEnd":274,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H0E2","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tollip also known as Toll-interacting protein acts as an important regulator in the immune response. This protein weighs approximately 30 kDa and is found expressed in several tissues especially in the lung liver and spleen. Tollip binds to various ubiquitinated proteins and plays a role in the endocytosis process assisting in protein trafficking. It functions by modulating the signaling pathways that involve innate immunity primarily by interacting with Toll-like receptors (TLRs).
Biological function summary

Toll-interacting protein participates in the negative regulation of the immune response preventing overactivation that could lead to inflammation. Tollip forms a complex with TLRs and IL-1 receptor pathway proteins to control inflammatory signaling. Through its action Tollip binds to Tom1 another protein that links Tollip to phosphatidylinositol-3-phosphate. By regulating these pathways Tollip assists in maintaining a balanced immune response.

Pathways

The protein engages in the TLR signaling pathway and the IL-1 receptor pathway. Both pathways are key in initiating immune responses against pathogens. Tollip as part of these pathways directly associates with proteins like IRAK-1 where it modulates the activity and ensures controlled signaling. This regulation prevents excessive or unnecessary activation that can result in immune-related damage.

Tollip links to conditions such as inflammatory bowel disease and sepsis. Both conditions involve significant immune dysregulation. In these disease states Tollip interacts with proteins like MyD88 affecting the response to pathogens and inflammatory stimuli. Malfunction or altered expression of Tollip can worsen symptoms or contribute to disease progression due to abnormal immune reactions.
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General info

Function

Component of the signaling pathway of IL-1 and Toll-like receptors (PubMed : 10854325, PubMed : 11751856). Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex (PubMed : 10854325). Inhibits IRAK1 phosphorylation and kinase activity (PubMed : 11751856). Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates (PubMed : 25042851). The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates (PubMed : 25042851). In a complex with TOM1, recruits ubiquitin-conjugated proteins onto early endosomes (PubMed : 15047686). Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed : 26320582).

Sequence similarities

Belongs to the tollip family.

Post-translational modifications

Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.

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Product protocols

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Target data

Component of the signaling pathway of IL-1 and Toll-like receptors (PubMed : 10854325, PubMed : 11751856). Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex (PubMed : 10854325). Inhibits IRAK1 phosphorylation and kinase activity (PubMed : 11751856). Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates (PubMed : 25042851). The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates (PubMed : 25042851). In a complex with TOM1, recruits ubiquitin-conjugated proteins onto early endosomes (PubMed : 15047686). Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P) (PubMed : 26320582).
See full target information TOLLIP
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