Recombinant Human Topoisomerase II alpha protein is a Human Fragment protein, in the 1435 to 1531 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
R A A P K G T K R D P A L N S G V S Q K P D P A K T K N R R K R K P S T S D D S D S N F E K I V S K A V T S K K S K G E S D D F H M D F D S A V A P R A K S V R A K K P I K Y L E E S D E D D L F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).
Alternative names
TOP2, TOP2A, DNA topoisomerase 2-alpha
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Recombinant Human Topoisomerase II alpha protein is a Human Fragment protein, in the 1435 to 1531 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P11388-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- R A A P K G T K R D P A L N S G V S Q K P D P A K T K N R R K R K P S T S D D S D S N F E K I V S K A V T S K K S K G E S D D F H M D F D S A V A P R A K S V R A K K P I K Y L E E S D E D D L F
- Accession
- P11388
- Protein length
- Fragment
- Amino acids
- 1435 to 1531
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).
- Sequence similarities
Belongs to the type II topoisomerase family.
- Post-translational modifications
Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation.
- Subcellular localisation
- Nucleus, Nucleoplasm, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Topoisomerase II alpha also known as TOP2A is an enzyme that plays a critical role in DNA replication and cell division. It is a homodimeric protein with a mass of around 170 kDa and primarily localizes in the nucleus. TOP2A catalyzes the transient breaking and rejoining of double-stranded DNA necessary for resolving DNA tangles and supercoils during the replication process. TOP2A is highly expressed in proliferating cells due to its role in cell cycle progression making it a significant target in cancer research.
- Biological function summary
TOP2A is essential for chromosome condensation segregation and relief of DNA torsional stress during mitosis. It does not function in isolation but interacts with other proteins as part of the larger DNA topoisomerase complex. This complex maintains DNA integrity during cellular processes such as transcription and replication. TOP2A's activity ensures that cells can duplicate their genetic material accurately facilitating proper cell division and maintaining genomic stability.
- Pathways
TOP2A plays a significant role in the DNA replication and cell cycle regulation pathways. It interacts closely with proteins like CDC2 (cyclin-dependent kinase 1) and is regulated by cyclins which control the timing of cell cycle progression. Additionally TOP2A is involved in the DNA damage response pathway where it collaborates with proteins such as ATM (ataxia-telangiectasia mutated) emphasizing its importance in maintaining DNA structure during replication stress.
- Associated diseases and disorders
Overexpression of TOP2A is frequently associated with various cancers including breast and prostate cancer. The enzyme's increased activity promotes uncontrolled cell proliferation a hallmark of cancer development. Antitopoisomerase drugs which target this pathway are used in cancer treatments to inhibit TOP2A activity. Additionally mutations or dysregulation of TOP2A-related pathways may also contribute to chemoresistance. Understanding TOP2A interactions and regulation provides insight into its role in cancer and opens avenues for targeted therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Human Topoisomerase II alpha protein (ab159735)
ab159735 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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