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AB206456

Recombinant human TPH2 protein

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Recombinant human TPH2 protein is a Human Fragment protein, in the 151 to 466 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

NTPH, TPH2, Tryptophan 5-hydroxylase 2, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

2 Images
Functional Studies - Recombinant human TPH2 protein (AB206456)
  • FuncS

Supplier Data

Functional Studies - Recombinant human TPH2 protein (AB206456)

Activity assay using ab206456

SDS-PAGE - Recombinant human TPH2 protein (AB206456)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human TPH2 protein (AB206456)

6-20% SDS-PAGE stained with Coomassie Blue.
Lane 1 : ab206456 (5 μg)
Lane 2 : Protein Marker

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Assay Conditions: Reaction was performed at room temperature in assay buffer containing 50 mM MOPS, pH 7.0, 60 μM tryptophan, 100 mM ammonium sulfate, 100 μM ferrous ammonium sulfate, 0.5 mM TCEP, 0.3 mM 6-methyl tetrahydropterin, 0.05 mg/ml catalase, 1 mM DTT and TPH2. Fluorescence signal was measured at Em360nm/Ex300nm.

Accession

Q8IWU9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.09% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"VPWFPRKISELDKCSHRVLMYGSELDADHPGFKDNVYRQRRKYFVDVAMGYKYGQPIPRVEYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYCGYREDNVPQLEDVSMFLKERSGFTVRPVAGYLSPRDFLAGLAYRVFHCTQYIRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCYFFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQEAYFVSESFEEAKEKMRDFAKSITRPFSVYFNPYTQSIEILKDTRSIENV","proteinLength":"Fragment","predictedMolecularWeight":"37 kDa","actualMolecularWeight":null,"aminoAcidEnd":466,"aminoAcidStart":151,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8IWU9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TPH2 protein also known as Tryptophan Hydroxylase 2 acts as a catalyst in the rate-limiting step of serotonin biosynthesis. It resides mostly in the brain specifically within neurons of the central nervous system where it facilitates the conversion of tryptophan to 5-hydroxytryptophan. TPH2 has a molecular mass of approximately 55 kDa. TPH2 expression is mainly observed in the raphe nuclei a region involved in mood regulation and other neural functions.
Biological function summary

TPH2 is essential for serotonin production impacting mood cognition and neurodevelopment. It functions independently rather than as part of a complex. By controlling serotonin levels TPH2 influences many physiological processes including sleep cycles appetite and emotional status. Its activity is important for maintaining normal levels of serotonin in the synaptic cleft therefore contributing to overall neural communication.

Pathways

TPH2 is involved in the serotonin biosynthesis pathway where it regulates serotonin production. This pathway is vital for mood and emotional regulation. TPH2 also interacts with enzymes like AADC (Aromatic L-amino acid decarboxylase) which further converts 5-hydroxytryptophan into serotonin. These interactions ensure a balanced production of serotonin essential for proper neuronal signaling and stable mental states.

TPH2 has connections with mental health conditions such as depression and anxiety disorders. Altered TPH2 function can lead to reduced serotonin levels contributing to the pathophysiology of these disorders. Studies show that TPH2 may interact with proteins like MAOA (Monoamine oxidase A) where mutations or dysregulation can exacerbate mood disorders. Understanding TPH2's role offers insights into therapeutic strategies targeting serotonin imbalances in mental health conditions.
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Specifications

Form

Liquid

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General info

Sequence similarities

Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

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Product protocols

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Target data

See full target information TPH2
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