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AB190993

Recombinant human TPM3 ALK protein

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(1 Publication)

Recombinant human TPM3 ALK protein is a Human Fragment protein, in the 1 to 258 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Tropomyosin alpha-3 chain, Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5, hTM5, TPM3

2 Images
Functional Studies - Recombinant human TPM3 ALK protein (AB190993)
  • FuncS

Supplier Data

Functional Studies - Recombinant human TPM3 ALK protein (AB190993)

The specific activitity of ab190993 was determined to be 30 nmol/ min/ mg.

SDS-PAGE - Recombinant human TPM3 ALK protein (AB190993)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human TPM3 ALK protein (AB190993)

The purity of ab190993 was determined to be >70% by densitometry, approx MW ~125 kDa.

Key facts

Purity

>70% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activitity of ab190993 was determined to be 30 nmol/ min/ mg.

Accession

P06753

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Recombinant fusion protein of TPM3 (1-258) - ALK (1058-1620) expressed in baculovirus in Sf9 insect cells using an N-terminal proprietary tag.
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Sequence info

[{"sequence":"MMEAIKKKMQMLKLDKENALDRAEQAEAEQKQAEERSKQLEDELAAMQKKLKGTEDELDKYSEALKDAQEKLELAEKKAADAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESKCSELEEELKNVTNNLKSLEAQAEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLE","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":258,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P06753","tags":[]},{"sequence":"VYRRKHQELQAMQMELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALIISKFNHQNIVRCIGVSLQSLPRFILLELMAGGDLKSFLRETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPGPGRVAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVYRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEKVPVRPKDPEGVPPLLVSQQAKREEERSPAAPPPLPTTSSGKAAKKPTAAEISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGSWFTEKPTKKNNPIAKKEPHDRGNLGLEGSCTVPPNVATGRLPGASLLLEPSSLTANMKEVPLFRLRHFPCGNVNYGYQQQGLPLEAATAPGAGHYEDTILKSKNSMNQPGP","proteinLength":"Fragment","predictedMolecularWeight":"125 kDa","actualMolecularWeight":null,"aminoAcidEnd":1620,"aminoAcidStart":1058,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9UM73","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPM3 ALK also known as Tropomyosin 3-Anaplastic Lymphoma Kinase is a fusion protein made by the joining of the TPM3 and ALK genes. The TPM3 part of the protein weighs about 32 kDa and it typically helps with muscle contraction while ALK is a receptor tyrosine kinase usually involved in cellular signaling and weighs approximately 162 kDa. TPM3 ALK results from a genetic rearrangement and this fusion protein is expressed in certain cancer cells particularly in some cases of anaplastic large cell lymphomas and inflammatory myofibroblastic tumors.
Biological function summary

This fusion protein acts as a constitutively active kinase meaning it remains active without normal regulation. The TPM3 ALK fusion circumvents regulatory mechanisms leading to unchecked cellular proliferation and survival. It doesn't involve participation in traditional cellular complexes but instead promotes tumorigenesis directly through its deregulated kinase activity.

Pathways

TPM3 ALK interacts significantly with signaling pathways that involve cell survival and proliferation. The fusion protein aberrantly activates the PI3K/AKT pathway and the RAS/MAPK pathway which are vital for cell growth and metabolic regulation. Through these pathways TPM3 ALK influences proteins such as STAT3 and PLCγ linking it further to the signaling network responsible for cell growth and division.

The presence of TPM3 ALK has strong links to specific types of cancer. Its expression is a marker for some anaplastic large cell lymphomas and certain inflammatory myofibroblastic tumors. The fusion between TPM3 and ALK creates a continual growth signal that parallels similar disease mechanisms seen with other ALK fusions like NPM1-ALK. This aberrant activation of ALK pathophysiology makes TPM3 ALK a potential target for therapeutic interventions aimed at inhibiting its kinase activity in affected patients.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Sequence similarities

Belongs to the tropomyosin family.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
See full target information Tropomyosin-3

Additional targets

ALK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational pediatrics 10:2083-2094 PubMed34584879

2021

Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Gedong Ming,Wanliang Guo,Yuan Cheng,Jian Wang
View all publications
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