Recombinant human TPM3 + TRKA protein is a Human Fragment protein, in the 1 to 258 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
M M E A I K K K M Q M L K L D K E N A L D R A E Q A E A E Q K Q A E E R S K Q L E D E L A A M Q K K L K G T E D E L D K Y S E A L K D A Q E K L E L A E K K A A D A E A E V A S L N R R I Q L V E E E L D R A Q E R L A T A L Q K L E E A E K A A D E S E R G M K V I E N R A L K D E E K M E L Q E I Q L K E A K H I A E E A D R K Y E E V A R K L V I I E G D L E R T E E R A E L A E S K C S E L E E E L K N V T N N L K S L E A Q A E K Y M M E A I K K K M Q M L K L D K E N A L D R A E Q A E A E Q K Q A E E R S K Q L E D E L A A M Q K K L K G T E D E L D K Y S E A L K D A Q E K L E L A E K K A A D A E A E V A S L N R R I Q L V E E E L D R A Q E R L A T A L Q K L E E A E K A A D E S E R G M K V I E N R A L K D E E K M E L Q E I Q L K E A K H I A E E A D R K Y E E V A R K L V I I E G D L E R T E E R A E L A E S K C S E L E E E L K N V T N N L K S L E A Q A E K Y S Q K E D K Y E E E I K I L T D K L K E A E T R A E F A E R S V A K L E K T I D D L E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1281417, PubMed:15488758, PubMed:17196528, PubMed:1849459, PubMed:1850821, PubMed:22649032, PubMed:27445338, PubMed:8325889). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. Isoform TrkA-III. Resistant to NGF, it constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
Additional Targets
TPM3
Alternative names
MTC, TRK, TRKA, NTRK1, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, Trk-A
Recommended products
Recombinant human TPM3 + TRKA protein is a Human Fragment protein, in the 1 to 258 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 12 nmol/min/mg.
- Accession
- P04629-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 1.71% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.001% PMSF
Sequence info
Amino acid sequence 1
- M M E A I K K K M Q M L K L D K E N A L D R A E Q A E A E Q K Q A E E R S K Q L E D E L A A M Q K K L K G T E D E L D K Y S E A L K D A Q E K L E L A E K K A A D A E A E V A S L N R R I Q L V E E E L D R A Q E R L A T A L Q K L E E A E K A A D E S E R G M K V I E N R A L K D E E K M E L Q E I Q L K E A K H I A E E A D R K Y E E V A R K L V I I E G D L E R T E E R A E L A E S K C S E L E E E L K N V T N N L K S L E A Q A E K Y M M E A I K K K M Q M L K L D K E N A L D R A E Q A E A E Q K Q A E E R S K Q L E D E L A A M Q K K L K G T E D E L D K Y S E A L K D A Q E K L E L A E K K A A D A E A E V A S L N R R I Q L V E E E L D R A Q E R L A T A L Q K L E E A E K A A D E S E R G M K V I E N R A L K D E E K M E L Q E I Q L K E A K H I A E E A D R K Y E E V A R K L V I I E G D L E R T E E R A E L A E S K C S E L E E E L K N V T N N L K S L E A Q A E K Y S Q K E D K Y E E E I K I L T D K L K E A E T R A E F A E R S V A K L E K T I D D L E
- Accession
- P06753
- Protein length
- Fragment
- Predicted molecular weight
- 105 kDa
- Amino acids
- 1 to 258
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Amino acid sequence 2
- D T N S T S G D P V E K K D E T P F G V S V A V G L A V F A C L F L S T L L L V L N K C G R R N K F G I N R P A V L A P E D G L A M S L H F M T L G G S S L S P T E G K G S G L Q G H I I E N P Q Y F S D A C V H H I K R R D I V L K W E L G E G A F G K V F L A E C H N L L P E Q D K M L V A V K A L K E A S E S A R Q D F Q R E A E L L T M L Q H Q H I V R F F G V C T E G R P L L M V F E Y M R H G D L N R F L R S H G P D A K L L A G G E D V A P G P L G L G Q L L A V A S Q V A A G M V Y L A G L H F V H R D L A T R N C L V G Q G L V V K I G D F G M S R D I Y S T D Y Y R V G G R T M L P I R W M P P E S I L Y R K F T T E S D V W S F G V V L W E I F T Y G K Q P W Y Q L S N T E A I D C I T Q G R E L E R P R A C P P E V Y A I M R G C W Q R E P Q Q R H S I K D V H A R L Q A L A Q A P P V Y L D V L G
- Accession
- P04629
- Protein length
- Fragment
- Amino acids
- 399 to 796
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1281417, PubMed:15488758, PubMed:17196528, PubMed:1849459, PubMed:1850821, PubMed:22649032, PubMed:27445338, PubMed:8325889). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
- Post-translational modifications
Ligand-mediated autophosphorylation (PubMed:1281417, PubMed:15488758, PubMed:27676246, PubMed:28177573, PubMed:2927393, PubMed:7510697, PubMed:8155326, PubMed:8325889). Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1 (PubMed:15488758, PubMed:7510697, PubMed:8155326, PubMed:8325889).
- Subcellular localisation
- Early endosome membrane, Late endosome membrane, Recycling endosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TPM3 also known as tropomyosin 3 and TRKA also referred to as NTRK1 are important proteins in cellular function. TPM3 is a low molecular weight protein of about 33 kDa. It is part of the tropomyosin family and stabilizes actin filaments in muscle fibers and non-muscle cells. TRKA is a receptor tyrosine kinase with a mass of approximately 87 kDa which localizes primarily on the cell membrane. TPM3 shows significant expression in muscle tissues while TRKA is present in neuronal tissues and certain peripheral tissues as well.
- Biological function summary
TPM3 supports the regulation of actin filament dynamics impacting muscle contraction and cell motility. In neurons TRKA is essential for nerve growth factor (NGF) binding influencing neurotrophic signaling pathways. Both proteins can exist within larger protein complexes contributing to their respective functions. TPM3 interacts with actin and other motor proteins whereas TRKA often forms complexes with adaptor proteins in receptor-mediated signaling.
- Pathways
TPM3 and TRKA each engage distinct yet critical cellular pathways. TPM3 integrates with the cytoskeletal pathway influencing cellular architecture and mechanical stability. TRKA on the other hand is pivotal in the MAPK/ERK and PI3K/AKT signaling pathways which regulate cell growth and survival. In this context TRKA interacts with proteins like SHC and GRB2 intermediaries in these signaling pathways.
- Associated diseases and disorders
TPM3 mutations can lead to congenital myopathies affecting muscle structure and function. Meanwhile TRKA is linked to certain cancers for instance neuroblastoma due to its role in cell proliferation and differentiation. In these conditions TRKA's interaction with ligands such as NGF has implications for tumor growth and treatment strategies.
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2 product images
SDS-PAGE - Recombinant human TPM3 + TRKA protein (ab191465)
SDS-PAGE analysis of ab191465
Functional Studies - Recombinant human TPM3 + TRKA protein (ab191465)
Specific Activity: Sample Kinase Activity Plot. The specific activity of ab191465 was determined to be 12 nmol /min/mg as per activity assay protocol.
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