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AB276511

Recombinant Human TPO protein (His tag)

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Recombinant Human TPO protein (His tag) is a Human Fragment protein, in the 1 to 846 aa range, expressed in Baculovirus infected insect cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Thyroid peroxidase, TPO

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SDS-PAGE - Recombinant Human TPO protein (His tag) (AB276511)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TPO protein (His tag) (AB276511)

SDS-PAGE analysis of ab276511

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P07202

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 10% Glycerol (glycerin, glycerine), 2.9% Sodium chloride, 0.24% Tris

storage-buffer

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRALAVLSVTLVMACTEAFFPFISRGKELLWGKPEESRVSSVLEESKRLVDTAMYATMQRNLKKRGILSPAQLLSFSKLPEPTSGVIARAAEIMETSIQAMKRKVNLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLPPKCPNTCLANKYRPITGACNNRDHPRWGASNTALARWLPPVYEDGFSQPRGWNPGFLYNGFPLPPVREVTRHVIQVSNEVVTDDDRYSDLLMAWGQYIDHDIAFTPQSTSKAAFGGGADCQMTCENQNPCFPIQLPEEARPAAGTACLPFYRSSAACGTGDQGALFGNLSTANPRQQMNGLTSFLDASTVYGSSPALERQLRNWTSAEGLLRVHARLRDSGRAYLPFVPPRAPAACAPEPGIPGETRGPCFLAGDGRASEVPSLTALHTLWLREHNRLAAALKALNAHWSADAVYQEARKVVGALHQIITLRDYIPRILGPEAFQQYVGPYEGYDSTANPTVSNVFSTAAFRFGHATIHPLVRRLDASFQEHPDLPGLWLHQAFFSPWTLLRGGGLDPLIRGLLARPAKLQVQDQLMNEELTERLFVLSNSSTLDLASINLQRGRDHGLPGYNEWREFCGLPRLETPADLSTAIASRSVADKILDLYKHPDNIDVWLGGLAENFLPRARTGPLFACLIGKQMKALRDGDWFWWENSHVFTDAQRRELEKHSLSRVICDNTGLTRVPMDAFQVGKFPEDFESCDSITGMNLEAWRETFPQDDKCGFPESVENGDFVHCEESGRRVLVYSCRHGYELQGREQLTCTQEGWDFQPPLCKDVNECADGAHPPCHASARCRNTKGGFQCLCADPYELGDDGRTCVDSGRLPR","proteinLength":"Fragment","predictedMolecularWeight":"93.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":846,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":null,"tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thyroid peroxidase (TPO) also known as thyroid microsomal antigen is an essential enzyme involved in the biosynthesis of thyroid hormones. TPO mechanistically catalyzes the iodination of tyrosine residues in thyroglobulin and the coupling of iodotyrosines to produce thyroxine (T4) and triiodothyronine (T3). This enzyme has a molecular mass approximately of 105 kDa and expresses mainly in the thyroid gland. Researchers often use anti-TPO antibodies to study this protein in scientific investigations because of its significant role in thyroid hormone production.
Biological function summary

TPO is integral in facilitating the thyroid gland's production of hormones necessary for metabolism. It operates in the thyroid follicular cells and works in conjunction with other components like hydrogen peroxide to ensure efficient hormone synthesis. TPO is not part of a larger complex but acts closely with the dual oxidase system that provides the hydrogen peroxide required for its enzymatic activity. This relationship is important for maintaining optimal levels of thyroid hormones necessary for various physiological functions.

Pathways

The biosynthesis of thyroid hormones is the primary pathway involving TPO. TPO plays a role along with thyroid-stimulating hormone receptor (TSHR) to regulate hormone synthesis and release. Another pathway the iodine metabolism pathway incorporates TPO's function to ensure that iodine an essential element effectively integrates into thyroglobulin. This integration is facilitated with the help of the sodium/iodide symporter (NIS) which allows the active transport of iodide ions into the thyroid cells.

TPO is closely related to autoimmune thyroid diseases (AITD) such as Hashimoto's thyroiditis and Graves' disease. These conditions often see the presence of anti-TPO antibodies which indicate an autoimmune response against TPO leading to thyroid dysfunction. A connection to these disorders can also involve the protein thyroglobulin (Tg) as both TPO and Tg are critical in thyroid hormone synthesis and are targets for autoantibodies in AITD. Understanding TPO's role helps in diagnosing and managing thyroid disorders effectively.
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Specifications

Form

Lyophilized

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General info

Function

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).

Sequence similarities

Belongs to the peroxidase family. XPO subfamily.

Post-translational modifications

Glycosylated.. Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.. Cleaved in its N-terminal part.

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Product protocols

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Target data

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
See full target information Thyroid peroxidase
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Product promise

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