Recombinant Human TPOR/MPL protein is a Human Fragment protein, in the 22 to 353 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
Q V S S Q D V S L L A S D S E P L K C F S R T F E D L T C F W D E E E A A P S G T Y Q L L Y A Y P R E K P R A C P L S S Q S M P H F G T R Y V C Q F P D Q E E V R L F F P L H L W V K N V F L N Q T R T Q R V L F V D S V G L P A P P S I I K A M G G S Q P G E L Q I S W E E P A P E I S D F L R Y E L R Y G P R D P K N S T G P T V I Q L I A T E T C C P A L Q R P H S A S A L D Q S P C A Q P T M P W Q D G P K Q T S P S R E A S A L T A E G G S C L I S G L Q P G N S Y W L Q L R S E P D G I S L G G S W G S W S L P V T V D L P G D A V A L G L Q C F T L D L K N V T C Q W Q Q Q D H A S S Q G F F Y H S R A R C C P R D R Y P I W E N C E E E E K T N P G L Q T P Q F S R C H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K (PubMed:15899890, PubMed:37633268). In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production.
Alternative names
CD110, TPOR, MPL, Thrombopoietin receptor, TPO-R, Myeloproliferative leukemia protein, Proto-oncogene c-Mpl
Recommended products
Recombinant Human TPOR/MPL protein is a Human Fragment protein, in the 22 to 353 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- No
- Accession
- P40238-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Q V S S Q D V S L L A S D S E P L K C F S R T F E D L T C F W D E E E A A P S G T Y Q L L Y A Y P R E K P R A C P L S S Q S M P H F G T R Y V C Q F P D Q E E V R L F F P L H L W V K N V F L N Q T R T Q R V L F V D S V G L P A P P S I I K A M G G S Q P G E L Q I S W E E P A P E I S D F L R Y E L R Y G P R D P K N S T G P T V I Q L I A T E T C C P A L Q R P H S A S A L D Q S P C A Q P T M P W Q D G P K Q T S P S R E A S A L T A E G G S C L I S G L Q P G N S Y W L Q L R S E P D G I S L G G S W G S W S L P V T V D L P G D A V A L G L Q C F T L D L K N V T C Q W Q Q Q D H A S S Q G F F Y H S R A R C C P R D R Y P I W E N C E E E E K T N P G L Q T P Q F S R C H
- Accession
- P40238
- Protein length
- Fragment
- Predicted molecular weight
- 37.1 kDa
- Actual molecular weight
- 37.1 kDa
- Amino acids
- 22 to 353
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K (PubMed:15899890, PubMed:37633268). In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production.
- Sequence similarities
Belongs to the type I cytokine receptor family. Type 1 subfamily.
- Post-translational modifications
Phosphorylated at Tyr-591 in response to THPO stimulation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target TPOR/MPL also known as the MPL protein or Myeloproliferative Leukemia protein functions as a receptor for thrombopoietin (TPO). It is composed of 635 amino acids with a molecular weight of approximately 70 kDa. This protein is expressed primarily on the surface of hematopoietic progenitor cells megakaryocytes and platelets. The receptor a cell surface-bound protein plays a significant role by binding to TPO facilitating cellular signaling involved in cell proliferation and maturation.
- Biological function summary
The TPOR/MPL protein promotes the growth and differentiation of multiple blood cell types through direct interactions with TPO. When TPO binds to MPL it triggers a signaling cascade essential for the regulation of platelet production and maintenance of hematopoiesis. This receptor forms part of a larger receptor complex that includes JAK2 protein kinase which further transduces signals for cell growth and survival. The engagement of JAK2 enhances the functional capability of MPL leading to the activation of various downstream effectors.
- Pathways
The TPOR/MPL protein is central to the thrombopoietin signaling pathway facilitating the activation of downstream molecules like STAT3 and STAT5. These transcription factors activate gene expression critical for cell proliferation and survival. MPL is closely related to other hematopoietic receptors within the Jak-STAT pathway. The pathway is integral for hematopoietic stem cell function and involves interactions with proteins like EPO receptor which utilizes similar pathways for erythropoiesis.
- Associated diseases and disorders
The TPOR/MPL protein is implicated in myeloproliferative disorders such as essential thrombocythemia and primary myelofibrosis. Mutations in the MPL gene can alter receptor function leading to uncontrolled cell proliferation. Such mutations often present in tandem with mutations in other proteins like JAK2 amplifying the disease processes. The inappropriate activation of the MPL signaling pathway results in excessive production of platelets or fibrosis within the bone marrow contributing significantly to disease phenotypes.
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3 product images
SDS-PAGE - Recombinant Human TPOR/MPL protein (ab310804)
SDS-PAGE analysis of ab310804
Mass Spectrometry - Recombinant Human TPOR/MPL protein (ab310804)
Mass determination by ESI-TOF. Predicted MW is 37124.96808 (+/- 10Da by ESI-TOF). Observed MW is 37127.15 +/-10Da.
HPLC - Recombinant Human TPOR/MPL protein (ab310804)
HPLC analysis of ab310804
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