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AB128435

Recombinant Human TPRKB protein (His tag N-Terminus)

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Recombinant Human TPRKB protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 175 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CGI-121, My019, TPRKB, EKC/KEOPS complex subunit TPRKB, PRPK-binding protein, TP53RK-binding protein

1 Images
SDS-PAGE - Recombinant Human TPRKB protein (His tag N-Terminus) (AB128435)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TPRKB protein (His tag N-Terminus) (AB128435)

Analysis of ab128435 (tagged Human TPRKB protein) on a 15% SDS-PAGE gel : Lane 1 : MWt marker
Lane 2 ab128435 at 3 µg

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9Y3C4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMQLTHQLDLFPECRVTLLLFKDVKNAGDLRRKAMEGTIDGSLINPTVIVDPFQILVAANKAVHLYKLGKMKTRTLSTEIIFNLSPNNNISEALKKFGISANDTSILIVYIEEGEKQINQEYLISQVEGHQVSLKNLPEIMNITEVKKIYKLSSQEESIGTLLDAIICRMSTKDVL","proteinLength":"Full Length","predictedMolecularWeight":"22.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":175,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y3C4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TPRKB also known as TP53RK-binding protein is a protein with a mass of approximately 39 kDa. It is expressed widely in various human tissues with notable levels seen in the testis. Mechanically TPRKB operates as part of the KEOPS/EKC complex which is essential for certain cellular processes. This complex participates in tRNA modifications and plays a role in maintaining certain chromosome structures.
Biological function summary

TPRKB contributes significantly to cell growth and survival. The KEOPS/EKC complex which includes TPRKB is involved in a range of cellular activities such as the addition of threonylcarbamoyl-AMP (t6A) to tRNAs. This modification ensures proper translation of the genetic code important for protein synthesis. Through these actions TPRKB assists in regulating cell cycle and cellular responses to stress. Proper functioning of tRNA modifications is essential for accurate protein translation impacting cellular homeostasis and division.

Pathways

TPRKB is integral to both the KEOPS/EKC pathway and the mTOR signaling pathway. The proper function of these pathways is important for nutrient sensing and regulation of cell growth. TPRKB interacts with other proteins such as TP53RK within the KEOPS/EKC complex. In the mTOR signaling pathway its role contributes to nutrient sensing and energy balance. These interactions hint at TPRKB's potential involvement in cellular metabolic regulation.

TPRKB has associations with certain cancers and growth-related abnormalities. It relates to cancer through its role in cell cycle regulation and genomic stability impacting tumorigenesis processes. The interaction of TPRKB with the protein TP53RK in the KEOPS/EKC complex may affect cell cycle control and tumor suppression. Additionally changes or mutations in TPRKB can link to growth disorders due to its critical functions in tRNA modification and cell growth pathways.
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Specifications

Form

Liquid

Additional notes

Purified by conventional chromatography techniques.

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General info

Function

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed : 22912744, PubMed : 28805828). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed : 22912744, PubMed : 28805828). TPRKB acts as an allosteric effector that regulates the t(6)A activity of the complex. TPRKB is not required for tRNA modification (PubMed : 22912744, PubMed : 28805828).

Sequence similarities

Belongs to the CGI121/TPRKB family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed : 22912744, PubMed : 28805828). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed : 22912744, PubMed : 28805828). TPRKB acts as an allosteric effector that regulates the t(6)A activity of the complex. TPRKB is not required for tRNA modification (PubMed : 22912744, PubMed : 28805828).
See full target information TPRKB
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