Recombinant Human TPRKB protein is a Human Full Length protein, in the 1 to 175 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M Q L T H Q L D L F P E C R V T L L L F K D V K N A G D L R R K A M E G T I D G S L I N P T V I V D P F Q I L V A A N K A V H L Y K L G K M K T R T L S T E I I F N L S P N N N I S E A L K K F G I S A N D T S I L I V Y I E E G E K Q I N Q E Y L I S Q V E G H Q V S L K N L P E I M N I T E V K K I Y K L S S Q E E S I G T L L D A I I C R M S T K D V L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:28805828). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:28805828). TPRKB acts as an allosteric effector that regulates the t(6)A activity of the complex. TPRKB is not required for tRNA modification (PubMed:22912744, PubMed:28805828).
Alternative names
CGI-121, My019, TPRKB, EKC/KEOPS complex subunit TPRKB, PRPK-binding protein, TP53RK-binding protein
Recommended products
Recombinant Human TPRKB protein is a Human Full Length protein, in the 1 to 175 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9Y3C4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M Q L T H Q L D L F P E C R V T L L L F K D V K N A G D L R R K A M E G T I D G S L I N P T V I V D P F Q I L V A A N K A V H L Y K L G K M K T R T L S T E I I F N L S P N N N I S E A L K K F G I S A N D T S I L I V Y I E E G E K Q I N Q E Y L I S Q V E G H Q V S L K N L P E I M N I T E V K K I Y K L S S Q E E S I G T L L D A I I C R M S T K D V L
- Accession
- Q9Y3C4
- Protein length
- Full Length
- Predicted molecular weight
- 22.2 kDa
- Amino acids
- 1 to 175
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified by conventional chromatography techniques.
General info
- Function
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:28805828). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:28805828). TPRKB acts as an allosteric effector that regulates the t(6)A activity of the complex. TPRKB is not required for tRNA modification (PubMed:22912744, PubMed:28805828).
- Sequence similarities
Belongs to the CGI121/TPRKB family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TPRKB also known as TP53RK-binding protein is a protein with a mass of approximately 39 kDa. It is expressed widely in various human tissues with notable levels seen in the testis. Mechanically TPRKB operates as part of the KEOPS/EKC complex which is essential for certain cellular processes. This complex participates in tRNA modifications and plays a role in maintaining certain chromosome structures.
- Biological function summary
TPRKB contributes significantly to cell growth and survival. The KEOPS/EKC complex which includes TPRKB is involved in a range of cellular activities such as the addition of threonylcarbamoyl-AMP (t6A) to tRNAs. This modification ensures proper translation of the genetic code important for protein synthesis. Through these actions TPRKB assists in regulating cell cycle and cellular responses to stress. Proper functioning of tRNA modifications is essential for accurate protein translation impacting cellular homeostasis and division.
- Pathways
TPRKB is integral to both the KEOPS/EKC pathway and the mTOR signaling pathway. The proper function of these pathways is important for nutrient sensing and regulation of cell growth. TPRKB interacts with other proteins such as TP53RK within the KEOPS/EKC complex. In the mTOR signaling pathway its role contributes to nutrient sensing and energy balance. These interactions hint at TPRKB's potential involvement in cellular metabolic regulation.
- Associated diseases and disorders
TPRKB has associations with certain cancers and growth-related abnormalities. It relates to cancer through its role in cell cycle regulation and genomic stability impacting tumorigenesis processes. The interaction of TPRKB with the protein TP53RK in the KEOPS/EKC complex may affect cell cycle control and tumor suppression. Additionally changes or mutations in TPRKB can link to growth disorders due to its critical functions in tRNA modification and cell growth pathways.
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SDS-PAGE - Recombinant Human TPRKB protein (ab128435)
Analysis of ab128435 (tagged Human TPRKB protein) on a 15% SDS-PAGE gel:
Lane 1: MWt marker
Lane 2 ab128435 at 3 µg
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