Recombinant Human TPST1 protein (His tag) is a Human Fragment protein, in the 26 to 370 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P Q H A M E C H H R I E E R S Q P V K L E S T R T T V R T G L D L K A N K T F A Y H K D M P L I F I G G V P R S G T T L M R A M L D A H P D I R C G E E T R V I P R I L A L K Q M W S R S S K E K I R L D E A G V T D E V L D S A M Q A F L L E I I V K H G E P A P Y L C N K D P F A L K S L T Y L S R L F P N A K F L L M V R D G R A S V H S M I S R K V T I A G F D L N S Y R D C L T K W N R A I E T M Y N Q C M E V G Y K K C M L V H Y E Q L V L H P E R W M R T L L K F L Q I P W N H S V L H H E E M I G K A G G V S L S K V E R S T D Q V I K P V N V G A L S K W V G K I P P D V L Q D M A V I A P M L A K L G Y D P Y A N P P N Y G K P D P K I I E N T R R V Y K G E F Q L P D F L K E K P Q T E Q V E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.
Alternative names
Protein-tyrosine sulfotransferase 1, Tyrosylprotein sulfotransferase 1, TPST-1, TPST1
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Recombinant Human TPST1 protein (His tag) is a Human Fragment protein, in the 26 to 370 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O60507-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P Q H A M E C H H R I E E R S Q P V K L E S T R T T V R T G L D L K A N K T F A Y H K D M P L I F I G G V P R S G T T L M R A M L D A H P D I R C G E E T R V I P R I L A L K Q M W S R S S K E K I R L D E A G V T D E V L D S A M Q A F L L E I I V K H G E P A P Y L C N K D P F A L K S L T Y L S R L F P N A K F L L M V R D G R A S V H S M I S R K V T I A G F D L N S Y R D C L T K W N R A I E T M Y N Q C M E V G Y K K C M L V H Y E Q L V L H P E R W M R T L L K F L Q I P W N H S V L H H E E M I G K A G G V S L S K V E R S T D Q V I K P V N V G A L S K W V G K I P P D V L Q D M A V I A P M L A K L G Y D P Y A N P P N Y G K P D P K I I E N T R R V Y K G E F Q L P D F L K E K P Q T E Q V E H H H H H H
- Accession
- O60507
- Protein length
- Fragment
- Predicted molecular weight
- 40.6 kDa
- Amino acids
- 26 to 370
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.
- Sequence similarities
Belongs to the protein sulfotransferase family.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TPST1 also known as tyrosylprotein sulfotransferase 1 plays an important role in the sulfation of tyrosine residues within proteins. This enzyme has an approximate mass of 50 kDa. TPST1 localizes mainly in the Golgi apparatus where it conducts its enzymatic activity. Expression of TPST1 occurs in various tissues with notable presence in platelets lung and liver.
- Biological function summary
TPST1 contributes to post-translational modification processes by facilitating the transfer of sulfate groups to proteins specifically on tyrosine residues. This modification influences protein-protein interactions and protein stability and occurs as a part of the protein sulfation complex. This complex comprises multiple enzymes working together to enhance the functionality of the target proteins impacting cellular communication and signaling.
- Pathways
TPST1 is involved in pathways associated with cell signaling and immune response. TPST1 contributes actively to the chemokine signaling pathway where it regulates the binding and activity of chemokines. This interaction frequently involves the CCR5 receptor an important element in immune responses and HIV infection pathways. Another significant pathway including TPST1 is G-protein coupled receptor signaling where its sulfation activity influences receptor binding and downstream effects.
- Associated diseases and disorders
TPST1 plays a role in disorders such as asthma and certain types of cancer. The enzyme can affect pathways linked to cell proliferation and inflammation and abnormal TPST1 activity associates with cancer progression particularly in conjunction with the protein CCR5. Understanding TPST1's role provides insight into the molecular basis of these diseases and helps identify potential targets for therapeutic intervention.
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1 product image
SDS-PAGE - Recombinant Human TPST1 protein (His tag) (ab222445)
ab222445 (3μg) was analysed by 15% SDS-PAGE.
Predicted molecular mass: 40.6 kDa
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