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AB119735

Recombinant Human TPX2 protein

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Recombinant Human TPX2 protein is a Human Full Length protein, in the 1 to 747 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB.

View Alternative Names

C20orf1, C20orf2, DIL2, HCA519, TPX2, Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, DIL-2, p100

1 Images
SDS-PAGE - Recombinant Human TPX2 protein (AB119735)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TPX2 protein (AB119735)

The purity was determined to be >80% by densitometry.

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

Q9ULW0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.7% Sodium phosphate, 0.003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Primary Antibodies

AB252944

Anti-TPX2 antibody [EPR23182-47]

RabMAb|Recombinant

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"110 kDa","actualMolecularWeight":null,"aminoAcidEnd":747,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9ULW0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Targeting Protein for Xklp2 (TPX2) also known as Microtubule-Associated Protein Required for Spindle acts mechanically in the assembly of mitotic spindles. With a molecular mass of approximately 82 kDa TPX2 localizes to the nucleus in interphase and distributes along the spindle microtubules during mitosis. Its expression is high in actively dividing cells such as those found in developing tissues and proliferative zones. This association with microtubules positions TPX2 as a critical player in cell division.
Biological function summary

TPX2 contributes to the proper regulation and progression of mitosis. As part of the mitotic spindle apparatus TPX2 interacts with other spindle assembly factors to facilitate the nucleation and stabilization of microtubules. It orchestrates the proper arrangement of spindle poles ensuring accurate chromosome segregation. TPX2 operates within a complex that includes proteins such as Aurora A kinase which further accentuates its role in spindle dynamics and mitotic progression.

Pathways

TPX2 interacts intricately with the cell cycle and mitotic spindle checkpoint pathways. As an important regulator in these pathways it works alongside proteins like Aurora A kinase and Eg5 to control spindle formation and function which are necessary for the G2/M phase transition. These pathways ensure that cell division proceeds only when all chromosomal alignments and spindle assemblies meet the required fidelity underlining the importance of TPX2 in maintaining genomic stability.

The overexpression of TPX2 often correlates with several types of cancer including breast and pancreatic cancers. Aberrant TPX2 levels disturb normal mitosis contributing to aneuploidy and tumor progression. Furthermore TPX2 overactivity can interact with oncogenic proteins such as MYC enhancing tumorigenic potential. Understanding TPX2's role in these diseases offers potential targets for therapeutic intervention making it an important subject of study in cancer research.
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Specifications

Form

Liquid

Additional notes

The purity was determined to be 80% by densitometry. Affinity purified.

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General info

Function

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed : 18663142, PubMed : 19208764, PubMed : 37728657). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed : 18663142, PubMed : 19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed : 26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activate AURKA kinase and stimulate local microtubule nucleation (PubMed : 26165940).

Sequence similarities

Belongs to the TPX2 family.

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Product protocols

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Target data

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed : 18663142, PubMed : 19208764, PubMed : 37728657). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed : 18663142, PubMed : 19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed : 26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activate AURKA kinase and stimulate local microtubule nucleation (PubMed : 26165940).
See full target information TPX2
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