Recombinant Human TPX2 protein is a Human Full Length protein, in the 1 to 747 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
>80% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, WB
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).
Alternative names
Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, DIL-2, p100, C20orf2, HCA519, DIL2, TPX2, C20orf1
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Recombinant Human TPX2 protein is a Human Full Length protein, in the 1 to 747 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
>80% SDS-PAGE
- Expression system
Baculovirus infected Sf9 cells
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, WB
- Biologically active
No
- Accession
- Q9ULW0-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.7% Sodium phosphate, 0.003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- Protein length
Full Length
- Predicted molecular weight
110 kDa
- Amino acids
1 to 747
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
The purity was determined to be 80% by densitometry. Affinity purified.
General info
- Function
Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).
- Sequence similarities
Belongs to the TPX2 family.
- Subcellular localisation
Nucleus, Cytoskeleton, Spindle, Spindle pole
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Targeting Protein for Xklp2 (TPX2) also known as Microtubule-Associated Protein Required for Spindle acts mechanically in the assembly of mitotic spindles. With a molecular mass of approximately 82 kDa TPX2 localizes to the nucleus in interphase and distributes along the spindle microtubules during mitosis. Its expression is high in actively dividing cells such as those found in developing tissues and proliferative zones. This association with microtubules positions TPX2 as a critical player in cell division.
- Biological function summary
TPX2 contributes to the proper regulation and progression of mitosis. As part of the mitotic spindle apparatus TPX2 interacts with other spindle assembly factors to facilitate the nucleation and stabilization of microtubules. It orchestrates the proper arrangement of spindle poles ensuring accurate chromosome segregation. TPX2 operates within a complex that includes proteins such as Aurora A kinase which further accentuates its role in spindle dynamics and mitotic progression.
- Pathways
TPX2 interacts intricately with the cell cycle and mitotic spindle checkpoint pathways. As an important regulator in these pathways it works alongside proteins like Aurora A kinase and Eg5 to control spindle formation and function which are necessary for the G2/M phase transition. These pathways ensure that cell division proceeds only when all chromosomal alignments and spindle assemblies meet the required fidelity underlining the importance of TPX2 in maintaining genomic stability.
- Associated diseases and disorders
The overexpression of TPX2 often correlates with several types of cancer including breast and pancreatic cancers. Aberrant TPX2 levels disturb normal mitosis contributing to aneuploidy and tumor progression. Furthermore TPX2 overactivity can interact with oncogenic proteins such as MYC enhancing tumorigenic potential. Understanding TPX2's role in these diseases offers potential targets for therapeutic intervention making it an important subject of study in cancer research.
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1 product image
SDS-PAGE - Recombinant Human TPX2 protein (ab119735)
The purity was determined to be >80% by densitometry.
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