Recombinant Human TRAF6 protein is a Human Full Length protein, in the 1 to 522 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M S L L N C E N S C G F S Q S E S D C C V A M A S S C S A V T K D D S V G G T A S T G N L S S S F M E E I Q G Y D V E F D P P L E S K Y E C P I C L M A L R E A V Q T P C G H R F C K A C I I K S I R D A G H K C P V D N E I L L E N Q L F P D N F A K R E I L S L M V K C P N E G C L H K M E L R H L E D H Q A H C E F A L M D C P Q C Q R P F Q K F H I N I H I L K D C P R R Q V S C D N C A A S M A F E D K E I H D Q N C P L A N V I C E Y C N T I L I R E Q M P N H Y D L D C P T A P I P C T F S T F G C H E K M Q R N H L A R H L Q E N T Q S H M R M L A Q A V H S L S V I P D S G Y I S E V R N F Q E T I H Q L E G R L V R Q D H Q I R E L T A K M E T Q S M Y V S E L K R T I R T L E D K V A E I E A Q Q C N G I Y I W K I G N F G M H L K C Q E E E K P V V I H S P G F Y T G K P G Y K L C M R L H L Q L P T A Q R C A N Y I S L F V H T M Q G E Y D S H L P W P F Q G T I R L T I L D Q S E A P V R Q N H E E I M D A K P E L L A F Q R P T I P R N P K G F G Y V T F M H L E A L R Q R T F I K D D T L L V R C E V S T R F D M G S L R R E G F Q P R S T D A G V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes (Recombinant protein). |
Target data
Function
E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as ECSIT, IKBKG, IRAK1, AKT1 and AKT2 (PubMed:11057907, PubMed:18347055, PubMed:19465916, PubMed:19713527, PubMed:31620128). Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation (PubMed:19675569). Leads to the activation of NF-kappa-B and JUN (PubMed:16378096, PubMed:17135271, PubMed:17703191). Seems to also play a role in dendritic cells (DCs) maturation and/or activation (By similarity). Represses c-Myb-mediated transactivation, in B-lymphocytes (PubMed:18093978, PubMed:18758450). Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor (PubMed:12140561, PubMed:19825828, PubMed:8837778). Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation (By similarity). Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production (By similarity). Participates also in the TCR signaling by ubiquitinating LAT (PubMed:23514740, PubMed:25907557).
Alternative names
RNF85, TRAF6, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6
Recommended products
Recombinant Human TRAF6 protein is a Human Full Length protein, in the 1 to 522 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q9Y4K3-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M S L L N C E N S C G F S Q S E S D C C V A M A S S C S A V T K D D S V G G T A S T G N L S S S F M E E I Q G Y D V E F D P P L E S K Y E C P I C L M A L R E A V Q T P C G H R F C K A C I I K S I R D A G H K C P V D N E I L L E N Q L F P D N F A K R E I L S L M V K C P N E G C L H K M E L R H L E D H Q A H C E F A L M D C P Q C Q R P F Q K F H I N I H I L K D C P R R Q V S C D N C A A S M A F E D K E I H D Q N C P L A N V I C E Y C N T I L I R E Q M P N H Y D L D C P T A P I P C T F S T F G C H E K M Q R N H L A R H L Q E N T Q S H M R M L A Q A V H S L S V I P D S G Y I S E V R N F Q E T I H Q L E G R L V R Q D H Q I R E L T A K M E T Q S M Y V S E L K R T I R T L E D K V A E I E A Q Q C N G I Y I W K I G N F G M H L K C Q E E E K P V V I H S P G F Y T G K P G Y K L C M R L H L Q L P T A Q R C A N Y I S L F V H T M Q G E Y D S H L P W P F Q G T I R L T I L D Q S E A P V R Q N H E E I M D A K P E L L A F Q R P T I P R N P K G F G Y V T F M H L E A L R Q R T F I K D D T L L V R C E V S T R F D M G S L R R E G F Q P R S T D A G V
- Accession
- Q9Y4K3
- Protein length
- Full Length
- Predicted molecular weight
- 86 kDa
- Amino acids
- 1 to 522
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as ECSIT, IKBKG, IRAK1, AKT1 and AKT2 (PubMed:11057907, PubMed:18347055, PubMed:19465916, PubMed:19713527, PubMed:31620128). Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation (PubMed:19675569). Leads to the activation of NF-kappa-B and JUN (PubMed:16378096, PubMed:17135271, PubMed:17703191). Seems to also play a role in dendritic cells (DCs) maturation and/or activation (By similarity). Represses c-Myb-mediated transactivation, in B-lymphocytes (PubMed:18093978, PubMed:18758450). Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor (PubMed:12140561, PubMed:19825828, PubMed:8837778). Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation (By similarity). Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production (By similarity). Participates also in the TCR signaling by ubiquitinating LAT (PubMed:23514740, PubMed:25907557).
- Sequence similarities
Belongs to the TNF receptor-associated factor family. A subfamily.
- Post-translational modifications
Sumoylated on Lys-124, Lys-142 and Lys-453 with SUMO1.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAF6 also known as TNF receptor-associated factor 6 is an E3 ubiquitin ligase that is part of the TRAF family. It has a molecular mass of approximately 60 kDa. TRAF6 is ubiquitously expressed in many types of tissues and cells including immune cells like macrophages and dendritic cells. Its primary role is to mediate signal transduction from members of the TNF receptor and Toll-like receptor (TLR) families which it achieves through polyubiquitination of target proteins. This process facilitates the recruitment of downstream signaling components necessary for cellular responses.
- Biological function summary
TRAF6 plays a critical role in regulating immune and inflammatory responses. It acts within a complex often associating with other signaling proteins such as IRAK1 and TAK1 to activate downstream kinases like IKK and JNK. These signaling pathways lead to the activation of transcription factors such as NF-kB and AP-1 which regulate the expression of genes involved in inflammation and immune responses. TRAF6 is an important modulator of adaptive and innate immunity influencing both the maturation of immune cells and the body's response to infection.
- Pathways
TRAF6 significantly contributes to the NF-kB and MAPK signaling pathways. In the NF-kB pathway TRAF6 mediates signal transduction following TLR engagement interacting with proteins such as MyD88 and RIP1 to drive inflammatory cytokine production. In the MAPK pathway it associates predominantly with TAK1 to facilitate the phosphorylation and activation of downstream kinases. These pathways are essential for transmitting signals from cell surface receptors to the nucleus controlling the magnitude of immune signaling and cellular stress responses.
- Associated diseases and disorders
TRAF6 has a significant association with autoimmune diseases and certain cancers. In autoimmune disorders aberrant activation of TRAF6 can lead to prolonged inflammation and detrimental immune responses. For cancers TRAF6 is linked to tumor progression and metastasis acting alongside proteins like IRAK1 and RIP1 which can further promote malignancy through chronic inflammation and immune evasion. Understanding TRAF6's role in these diseases provides insights that could lead to targeted therapeutic strategies.
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1 product image
SDS-PAGE - Recombinant Human TRAF6 protein (ab132023)
12.5% SDS-PAGE stained with Coomassie Blue showing ab132023 at approximately 86 kDa.
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