Recombinant Human TRAIL protein is a Human Fragment protein, in the 114 to 281 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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- Cell metabolism 33:1124-1136.e52021Oxylipin biosynthesis reinforces cellular senescence and allows detection of senolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher D Wiley,Rishi Sharma,Sonnet S Davis,Jose Alberto Lopez-Dominguez,Kylie P Mitchell,Samantha Wiley,Fatouma Alimirah,Dong Eun Kim,Therese Payne,Andrew Rosko,Eliezer Aimontche,Sharvari M Deshpande,Francesco Neri,Chisaka Kuehnemann,Marco Demaria,Arvind Ramanathan,Judith CampisiPubMed 33811820
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M V R E R G P Q R V A A H I T G T R G R S N T L S S P N S K N E K A L G R K I N S W E S S R S G H S F L S N L H L R N G E L V I H E K G F Y Y I Y S Q T Y F R F Q E E I K E N T K N D K Q M V Q Y I Y K Y T S Y P D P I L L M K S A R N S C W S K D A E Y G L Y S I Y Q G G I F E L K E N D R I F V S V T N E H L I D M D H E A S F F G A F L V G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:10549288, PubMed:26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Alternative names
CD253, APO2L, TRAIL, TNFSF10, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, TNF-related apoptosis-inducing ligand, Apo-2L, Protein TRAIL
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Recombinant Human TRAIL protein is a Human Fragment protein, in the 114 to 281 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P50591-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.242% Tris, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M V R E R G P Q R V A A H I T G T R G R S N T L S S P N S K N E K A L G R K I N S W E S S R S G H S F L S N L H L R N G E L V I H E K G F Y Y I Y S Q T Y F R F Q E E I K E N T K N D K Q M V Q Y I Y K Y T S Y P D P I L L M K S A R N S C W S K D A E Y G L Y S I Y Q G G I F E L K E N D R I F V S V T N E H L I D M D H E A S F F G A F L V G
- Accession
- P50591
- Protein length
- Fragment
- Amino acids
- 114 to 281
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab78818 is purified using conventional chromatography techniques.
General info
- Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:10549288, PubMed:26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
- Sequence similarities
Belongs to the tumor necrosis factor family.
- Post-translational modifications
Tyrosine phosphorylated by PKDCC/VLK.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.
- Biological function summary
TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.
- Pathways
TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.
- Associated diseases and disorders
TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.
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Sandwich ELISA - Recombinant Human TRAIL protein (ab78818)
Standard curve for TRAIL (Analyte: ab78818); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [55B709.3] to TRAIL (ab12124) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to TRAIL (Anti-TRAIL antibody ab9959) at 0.1μg/ml.
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