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AB83560

Recombinant human Transferrin protein

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Recombinant human Transferrin protein is a Human Full Length protein, expressed in HEK 293 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PRO1400, TF, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin

4 Images
Functional Studies - Recombinant human Transferrin protein (AB83560)
  • FuncS

Unknown

Functional Studies - Recombinant human Transferrin protein (AB83560)

Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates that ab83560 exists in multiple isoforms, which differ according to their level of posttranslational modification. Expression of these isoforms is highly significant for cell biology, as they more closely resemble the native human proteins. The triangle indicates theoretical pI and MW of the protein.

Sandwich ELISA - Recombinant human Transferrin protein (AB83560)
  • sELISA

Unknown

Sandwich ELISA - Recombinant human Transferrin protein (AB83560)

Standard curve for Transferrin (Analyte : ab83560); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [HTF-14] to Transferrin (ab769) at 1μg/ml and Detector Antibody Rabbit polyclonal to Transferrin (ab9538) at 0.5μg/ml.

SDS-PAGE - Recombinant human Transferrin protein (AB83560)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Transferrin protein (AB83560)

A sample of ab83560 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris HCl 2D gel. Approximately 40 μg of protein was load; Gel was stained using Deep Purple™. Spot train indicates presence of multiple isoforms of Transferrin. Spots within the spot train were cut from the gel and identified as Transferrin by protein mass fingerprinting.

SDS-PAGE - Recombinant human Transferrin protein (AB83560)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Transferrin protein (AB83560)

Lane 1 – MW markers; Lane 2 – ab83560; Lane 3 – ab83560 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83560 treated with a glycosidase cocktail to remove potential N- and Olinked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.

Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A further drop in MW after treatment with the glycosidase cocktail indicates the presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.

Key facts

Purity

>95% SDS-PAGE

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The activity of ab83560 was measured by its ability to support the growth of HepG2 cells under conditions of reduced serum. Typically 0.1 to 1 μg enhances cell proliferation.

Accession

P02787

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in 0.5 mL of PBS

Storage buffer

Constituents: 10% Trehalose, 1% Human serum albumin

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB116027

Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa) (AB116027)

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Primary Antibodies

AB109503

Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control

RabMAb|Recombinant|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (AB109503)

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Assay Kits

AB187391

Human Transferrin ELISA Kit

SimpleStep

ELISA - Human Transferrin ELISA Kit (AB187391)

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Proteins & Peptides

AB322088

Recombinant Human Transferrin Receptor Protein Standard (His tag)

Sandwich ELISA - Recombinant Human Transferrin Receptor Protein Standard (His tag) (AB322088)

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Proteins & Peptides

AB78877

Recombinant Human Ferritin Heavy Chain protein (Tag Free)

Western blot - Recombinant Human Ferritin Heavy Chain protein (Tag Free) (AB78877)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Under reducing conditions this protein migrates as a broad band between 70-80 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified form that has a predicted MW of 75.2 kDa. ab83560 consists of 5-20% carbohydrate by weight. This protein separates into a number of isoforms with a pI between 6.0-8.0 in 2D PAGE due to modifications, in particular glycosylation. This compares with the unmodified form that has a predicted pI of 6.8.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"Theoretical sequence: \r\nVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASY\r\nLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKED\r\nPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLL\r\nYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCS\r\nTLNQYFGYSGAFKCLKNGAGDVAFVKHSTIFENLANKADRDQYEL\r\nLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQA\r\nQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYL\r\nGYEYVTAIRNLREGTCQEAPTDECKPVKWCALSHHERLKCDEWSV\r\nNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLV\r\nPVLAENYNKSDNCEDTPEAGYFAVAVVKKSASDLTWDNLKGKKSC\r\nHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLC\r\nKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQ\r\nNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNH\r\nAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLL\r\nFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACT\r\nFRRP","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P02787","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Reconstitute for long term storage
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.
Biological function summary

Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.

Pathways

Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.

Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.
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Specifications

Form

Lyophilized

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General info

Function

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6 : ESAG7 and extract its iron for its own use.

Sequence similarities

Belongs to the transferrin family.

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Product protocols

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Target data

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6 : ESAG7 and extract its iron for its own use.
See full target information TF
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