Recombinant human TRAP/CD40L protein (Animal Free) is a Human Full Length protein, in the 113 to 261 aa range, expressed in Escherichia coli, with >98% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.
>98% SDS-PAGE
< 0.1 EU/µg
Escherichia coli
Tag free
SDS-PAGE, FuncS, HPLC
Yes
M Q K G D Q N P Q I A A H V I S E A S S K T T S V L Q W A E K G Y Y T M S N N L V T L E N G K Q L T V K R Q G L Y Y I Y A Q V T F C S N R E A S S Q A P F I A S L W L K S P G R F E R I L L R A A N T H S S A K P C G Q Q S I H L G G V F E L Q P G A S V F V N V T D P S Q V S H G T G F T S F G L L K L
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).CD40 ligand, soluble formActs as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, TRAP, TRAP, TNFSF5, CD40L, CD40LG
Recombinant human TRAP/CD40L protein (Animal Free) is a Human Full Length protein, in the 113 to 261 aa range, expressed in Escherichia coli, with >98% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.
CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, TRAP, TRAP, TNFSF5, CD40L, CD40LG
>98% SDS-PAGE
< 0.1 EU/µg
Escherichia coli
Tag free
SDS-PAGE, FuncS, HPLC
Yes
Determined by its ability to stimulate the proliferation of HEK-Blue™ CD40 reporter cells.
Yes
Human
Lyophilised from a sterile filtered solution. Centrifuge the vial prior to opening. Reconstitute in Water to a concentration of 0.1 - 1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C
M Q K G D Q N P Q I A A H V I S E A S S K T T S V L Q W A E K G Y Y T M S N N L V T L E N G K Q L T V K R Q G L Y Y I Y A Q V T F C S N R E A S S Q A P F I A S L W L K S P G R F E R I L L R A A N T H S S A K P C G Q Q S I H L G G V F E L Q P G A S V F V N V T D P S Q V S H G T G F T S F G L L K L
Full Length
16.3 kDa
113 to 261
Recombinant
Lyophilized
assessed also by HPLC
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).
Belongs to the tumor necrosis factor family.
The soluble form derives from the membrane form by proteolytic processing.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.
The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.
CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.
CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.
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