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AB181920

Recombinant Human TRBP protein (denatured) (Tag Free)

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(1 Publication)

Recombinant Human TRBP protein (denatured) (Tag Free) is a Human Full Length protein, in the 1 to 345 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

TRBP, TARBP2, RISC-loading complex subunit TARBP2, TAR RNA-binding protein 2, Trans-activation-responsive RNA-binding protein

1 Images
SDS-PAGE - Recombinant Human TRBP protein (denatured) (Tag Free) (AB181920)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TRBP protein (denatured) (Tag Free) (AB181920)

15% SDS PAGE analysis of ab181920 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q15633

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGDTSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSPLDSSLPEDIPVFTAAAAATPVPSVVLTRSPPMELQPPVSPQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFIEIGSGTSKKLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLRSCSLGSLGALGPACCRVLSELSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCHGSATTREAARGEAARRALQYLKIMAGSK","proteinLength":"Full Length","predictedMolecularWeight":"36.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":345,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15633","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transactivation response RNA-binding protein (TRBP) also known as TARBP2 is a protein with a molecular weight of approximately 39 kDa. It is widely expressed in various tissues and plays a role in the RNA interference (RNAi) pathway. TRBP binds to double-stranded RNA and aids in the processing of microRNA (miRNA) by interacting with proteins such as Dicer and Argonaute. This protein participates significantly in the regulation of gene expression by influencing the stability and translation of target mRNAs.
Biological function summary

TRBP acts as a section of the RNA-induced silencing complex (RISC) where it directly interacts with Dicer and facilitates the maturation of miRNA precursors into their active forms. Through these interactions TRBP helps control diverse biological processes including cell growth differentiation and apoptosis. Its role in miRNA processing highlights its importance in maintaining cellular homeostasis. TRBP’s interactions extend beyond Dicer as it also stabilizes protein structures through its ability to homodimerize.

Pathways

TRBP contributes to the miRNA-mediated gene silencing pathway and the RNAi pathway. It works closely with proteins such as Dicer and Argonaute to ensure the effective formation of miRNA mature strands. TRBP's participation in these pathways links it with key cellular processes and suggests its implication in gene regulatory networks. This functional involvement with miRNA highlights TRBP's role in regulating post-transcriptional gene silencing adding layers of control over gene expression that are critical to normal cellular function.

TRBP has associations with cancer and neurological disorders. In cancer TRBP can regulate tumor suppressor miRNAs and oncogenic miRNAs influencing tumor progression and metastasis. TRBP is also connected to Neurological disorders where its dysregulation may affect neuronal gene expression. These connections position TRBP as a significant player in pathological conditions and stress the importance of understanding its role for potential therapeutic strategies.
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Specifications

Form

Liquid

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General info

Function

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1 (By similarity) (PubMed : 15973356, PubMed : 16142218, PubMed : 16271387, PubMed : 16357216, PubMed : 16424907, PubMed : 17452327, PubMed : 18178619). Binds in vitro to the PRM1 3'-UTR (By similarity). Seems to act as a repressor of translation (By similarity). For some pre-miRNA substrates, may also alter the choice of cleavage site by DICER1 (PubMed : 23063653). Negatively regulates IRF7-mediated IFN-beta signaling triggered by viral infection by inhibiting the phosphorylation of IRF7 and promoting its 'Lys'-48-linked ubiquitination and degradation (PubMed : 30927622).. (Microbial infection) Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs (PubMed : 11438532, PubMed : 12475984, PubMed : 2011739). This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha (PubMed : 11438532). May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR (PubMed : 12475984). Mediates recruitment of FTSJ3 methyltransferase to HIV-1 RNA, leading to 2'-O-methylation of the viral genome, allowing HIV-1 to escape the innate immune system (PubMed : 30626973).

Sequence similarities

Belongs to the TARBP2 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1 (By similarity) (PubMed : 15973356, PubMed : 16142218, PubMed : 16271387, PubMed : 16357216, PubMed : 16424907, PubMed : 17452327, PubMed : 18178619). Binds in vitro to the PRM1 3'-UTR (By similarity). Seems to act as a repressor of translation (By similarity). For some pre-miRNA substrates, may also alter the choice of cleavage site by DICER1 (PubMed : 23063653). Negatively regulates IRF7-mediated IFN-beta signaling triggered by viral infection by inhibiting the phosphorylation of IRF7 and promoting its 'Lys'-48-linked ubiquitination and degradation (PubMed : 30927622).. (Microbial infection) Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs (PubMed : 11438532, PubMed : 12475984, PubMed : 2011739). This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha (PubMed : 11438532). May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR (PubMed : 12475984). Mediates recruitment of FTSJ3 methyltransferase to HIV-1 RNA, leading to 2'-O-methylation of the viral genome, allowing HIV-1 to escape the innate immune system (PubMed : 30626973).
See full target information TARBP2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0120614 PubMed25793518

2015

DICER-ARGONAUTE2 complex in continuous fluorogenic assays of RNA interference enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Mark A Bernard,Leyu Wang,Souvenir D Tachado
View all publications
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