Recombinant Human TRIB1 protein is a Human Full Length protein, in the 1 to 372 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M R V G P V R S A M S G A S Q P R G P A L L F P A T R G V P A K R L L D A D D A A A V A A K C P R L S E C S S P P D Y L S P P G S P C S P Q P P P A A P G A G G G S G S A P G P S R I A D Y L L L P L A E R E H V S R A L C I H T G R E L R C K V F P I K H Y Q D K I R P Y I Q L P S H S N I T G I V E V I L G E T K A Y V F F E K D F G D M H S Y V R S R K R L R E E E A A R L F K Q I V S A V A H C H Q S A I V L G D L K L R K F V F S T E E R T Q L R L E S L E D T H I M K G E D D A L S D K H G C P A Y V S P E I L N T T G T Y S G K A A D V W S L G V M L Y T L L V G R Y P F H D S D P S A L F S K I R R G Q F C I P E H I S P K A R C L I R S L L R R E P S E R L T A P E I L L H P W F E S V L E P G Y I D S E I G T S D Q I V P E Y Q E D S D I S S F F C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Adapter protein involved in protein degradation by interacting with COP1 ubiquitin ligase (PubMed:27041596). The COP1-binding motif is masked by autoinhibitory interactions with the protein kinase domain (PubMed:26455797). Serves to alter COP1 substrate specificity by directing the activity of COP1 toward CEBPA (PubMed:27041596). Binds selectively the recognition sequence of CEBPA (PubMed:26455797). Regulates myeloid cell differentiation by altering the expression of CEBPA in a COP1-dependent manner (By similarity). Controls macrophage, eosinophil and neutrophil differentiation via the COP1-binding domain (By similarity). Interacts with MAPK kinases and regulates activation of MAP kinases, but has no kinase activity (PubMed:15299019, PubMed:26455797).
Alternative names
C8FW, GIG2, TRB1, TRIB1, Tribbles homolog 1, TRB-1, G-protein-coupled receptor-induced gene 2 protein, SKIP1, GIG-2
Recommended products
Recombinant Human TRIB1 protein is a Human Full Length protein, in the 1 to 372 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- Q96RU8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M R V G P V R S A M S G A S Q P R G P A L L F P A T R G V P A K R L L D A D D A A A V A A K C P R L S E C S S P P D Y L S P P G S P C S P Q P P P A A P G A G G G S G S A P G P S R I A D Y L L L P L A E R E H V S R A L C I H T G R E L R C K V F P I K H Y Q D K I R P Y I Q L P S H S N I T G I V E V I L G E T K A Y V F F E K D F G D M H S Y V R S R K R L R E E E A A R L F K Q I V S A V A H C H Q S A I V L G D L K L R K F V F S T E E R T Q L R L E S L E D T H I M K G E D D A L S D K H G C P A Y V S P E I L N T T G T Y S G K A A D V W S L G V M L Y T L L V G R Y P F H D S D P S A L F S K I R R G Q F C I P E H I S P K A R C L I R S L L R R E P S E R L T A P E I L L H P W F E S V L E P G Y I D S E I G T S D Q I V P E Y Q E D S D I S S F F C
- Accession
- Q96RU8
- Protein length
- Full Length
- Predicted molecular weight
- 70 kDa
- Amino acids
- 1 to 372
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Adapter protein involved in protein degradation by interacting with COP1 ubiquitin ligase (PubMed:27041596). The COP1-binding motif is masked by autoinhibitory interactions with the protein kinase domain (PubMed:26455797). Serves to alter COP1 substrate specificity by directing the activity of COP1 toward CEBPA (PubMed:27041596). Binds selectively the recognition sequence of CEBPA (PubMed:26455797). Regulates myeloid cell differentiation by altering the expression of CEBPA in a COP1-dependent manner (By similarity). Controls macrophage, eosinophil and neutrophil differentiation via the COP1-binding domain (By similarity). Interacts with MAPK kinases and regulates activation of MAP kinases, but has no kinase activity (PubMed:15299019, PubMed:26455797).
- Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRIB1 also known as tribbles homolog 1 is a pseudo kinase protein with a mass of approximately 41 kDa. It lacks catalytic activity typically expected from kinases but it plays a critical mechanical role via its protein interactions. TRIB1 is expressed in various human tissues including liver spleen and heart. It acts mainly through modulating protein degradation and transcription regulation processes.
- Biological function summary
TRIB1 impacts cell signaling and metabolic processes by forming complexes with other proteins. It exerts a regulatory role by binding to E3 ubiquitin ligases influencing the degradation of target proteins. TRIB1 also interacts with transcription factors affecting gene expression. These actions highlight its involvement in maintaining cellular homeostasis by controlling inflammatory responses and metabolic pathways.
- Pathways
TRIB1 influences metabolic and signaling pathways by interacting with key regulatory proteins. It plays a significant role within the MAP kinase pathway and the PI3K-AKT pathway. Both pathways are important for cell proliferation survival and metabolism. In these pathways proteins like ATF4 in the MAP kinase pathway and AKT1 in the PI3K-AKT pathway associate with TRIB1 allowing it to exert control over important cellular processes.
- Associated diseases and disorders
TRIB1 is linked to conditions such as cardiovascular disease and certain cancers. Its regulatory function within metabolic pathways connects it to lipid metabolism disorders impacting cardiovascular health. TRIB1 also relates to leukemia through its interactions with the transcription factor C/EBPα. Dysregulation of TRIB1 expression or function can disrupt normal cellular functions leading to disease progression.
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SDS-PAGE - Recombinant Human TRIB1 protein (ab186079)
SDS-PAGE of ab186079.
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