Recombinant Human TRIB3 protein (denatured) is a Human Full Length protein, in the 1 to 358 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M R A T P L A A P A G S L S R K K R L E L D D N L D T E R P V Q K R A R S G P Q P R L P P C L L P L S P P T A P D R A T A V A T A S R L G P Y V L L E P E E G G R A Y Q A L H C P T G T E Y T C K V Y P V Q E A L A V L E P Y A R L P P H K H V A R P T E V L A G T Q L L Y A F F T R T H G D M H S L V R S R H R I P E P E A A V L F R Q M A T A L A H C H Q H G L V L R D L K L C R F V F A D R E R K K L V L E N L E D S C V L T G P D D S L W D K H A C P A Y V G P E I L S S R A S Y S G K A A D V W S L G V A L F T M L A G H Y P F Q D S E P V L L F G K I R R G A Y A L P A G L S A P A R C L V R C L L R R E P A E R L T A T G I L L H P W L R Q D P M P L A P T R S H L W E A A Q V V P D G L G L D E A R E E E G D R E V V L Y G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed:15781252, PubMed:15775988). Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (PubMed:15781252, PubMed:15775988). May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (PubMed:15781252, PubMed:15775988). Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (By similarity). Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (By similarity). May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (By similarity). Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (PubMed:12736262). Interacts with MAPK kinases and regulates activation of MAP kinases (PubMed:15299019). Can inhibit APOBEC3A editing of nuclear DNA (PubMed:22977230).
Alternative names
Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, SINK, p65-interacting inhibitor of NF-kappa-B, SKIP3, TRIB3, C20orf97, NIPK, TRB3
Recommended products
Recombinant Human TRIB3 protein (denatured) is a Human Full Length protein, in the 1 to 358 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Alternative names
Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, SINK, p65-interacting inhibitor of NF-kappa-B, SKIP3, TRIB3, C20orf97, NIPK, TRB3
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q96RU7-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M R A T P L A A P A G S L S R K K R L E L D D N L D T E R P V Q K R A R S G P Q P R L P P C L L P L S P P T A P D R A T A V A T A S R L G P Y V L L E P E E G G R A Y Q A L H C P T G T E Y T C K V Y P V Q E A L A V L E P Y A R L P P H K H V A R P T E V L A G T Q L L Y A F F T R T H G D M H S L V R S R H R I P E P E A A V L F R Q M A T A L A H C H Q H G L V L R D L K L C R F V F A D R E R K K L V L E N L E D S C V L T G P D D S L W D K H A C P A Y V G P E I L S S R A S Y S G K A A D V W S L G V A L F T M L A G H Y P F Q D S E P V L L F G K I R R G A Y A L P A G L S A P A R C L V R C L L R R E P A E R L T A T G I L L H P W L R Q D P M P L A P T R S H L W E A A Q V V P D G L G L D E A R E E E G D R E V V L Y G
- Accession
- Protein length
Full Length
- Predicted molecular weight
42 kDa
- Amino acids
1 to 358
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab202154 was purified by conventional chromatography techniques.
General info
- Function
Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed:15781252, PubMed:15775988). Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (PubMed:15781252, PubMed:15775988). May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (PubMed:15781252, PubMed:15775988). Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (By similarity). Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (By similarity). May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (By similarity). Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (PubMed:12736262). Interacts with MAPK kinases and regulates activation of MAP kinases (PubMed:15299019). Can inhibit APOBEC3A editing of nuclear DNA (PubMed:22977230).
- Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRIB3 also known as TRB3 or NIPK is a pseudokinase with a molecular mass of approximately 42 kDa. This protein does not possess catalytic activity. It functions largely through interactions with other proteins. TRIB3 is expressed in various tissues with high levels in the liver kidney and brain. The regulatory functions of TRIB3 involve modulating cellular processes such as stress response and apoptosis by interacting with signaling proteins.
- Biological function summary
The modulation of metabolic pathways and stress responses involves TRIB3 acting in several mechanisms. While TRIB3 itself is not part of a complex it exerts its effects by binding to and inhibiting the activity of other proteins notably AKT1 and several transcription factors. This modulation influences glucose metabolism and cellular response to unfolded protein stress indicating TRIB3's role as a significant regulator of cellular homeostasis.
- Pathways
The influence of TRIB3 on insulin signaling and the unfolded protein response is significant. Within the insulin signaling pathway TRIB3 interacts with AKT1 a central protein that regulates glucose uptake and cell survival. This interaction inhibits AKT1's activity impacting glucose metabolism. In the unfolded protein response pathway TRIB3 impacts stress response regulation through interactions that modulate transcriptional activity further highlighting its regulatory roles.
- Associated diseases and disorders
Alterations in TRIB3 expression and activity relate to conditions such as type 2 diabetes and cancer. In type 2 diabetes increased TRIB3 levels impair insulin signaling via AKT1 inhibition contributing to insulin resistance. In cancer the dysregulation of TRIB3 can promote survival pathways circumventing apoptosis and aiding in tumor progression. The involvement of TRIB3 in these diseases highlights its potential impact in therapeutic targeting.
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1 product image
SDS-PAGE - Recombinant Human TRIB3 protein (denatured) (ab202154)
15% SDS-PAGE analysis of ab202154 (3μg).
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