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AB153605

Recombinant Human TRIF protein (GST tag N-Terminus)

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Recombinant Human TRIF protein (GST tag N-Terminus) is a Human Fragment protein, in the 1 to 110 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

PRVTIRB, TRIF, TICAM1, TIR domain-containing adapter molecule 1, TICAM-1, Putative NF-kappa-B-activating protein 502H, Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta, MyD88-3, TIR domain-containing adapter protein inducing IFN-beta

1 Images
SDS-PAGE - Recombinant Human TRIF protein (GST tag N-Terminus) (AB153605)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human TRIF protein (GST tag N-Terminus) (AB153605)

ab153605 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q8IUC6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB13810

Anti-TRIF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIF antibody (AB13810)

  • 3
  • 6
Primary Antibodies

AB33915

Anti-TRAF6 antibody [EP591Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody [EP591Y] (AB33915)

  • 4
  • 9
Primary Antibodies

AB22048

Anti-TLR4 antibody [76B357.1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR4 antibody [76B357.1] (AB22048)

  • 4
  • 29
Primary Antibodies

AB16502

Anti-NF-kB p65 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody (AB16502)

  • 4
  • 40
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"AIDHSAFIILLLTSNFDCRLSLHQVNQAMMSNLTRQGSPDCVIPFLPLESSPAQLSSDTASLLSGLVRLDEHSQIFARKVANTFKPHRLQARKAMWRKEQDTRALREQSQ","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":110,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8IUC6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIF also known as TIR-domain-containing adapter-inducing interferon-β is an adapter protein that plays an important role in the immune response. It has a molecular mass of about 85 kDa. TRIF is expressed in various tissues but most highly in immune cells like macrophages and dendritic cells. Mechanically TRIF acts within the signaling pathways by connecting Toll-like receptors (TLR) with downstream signaling molecules. It is essential for activating pathways that lead to the production of type I interferons and other cytokines.
Biological function summary

TRIF is part of the innate immune response mechanism. It functions as a central component in signal transduction processes especially in response to pathogen-associated molecular patterns (PAMPs). TRIF often forms complexes with other signaling proteins to activate transcription factors such as IRF3 and NF-kB which stimulate the expression of inflammatory cytokines and antiviral genes. This function allows the body to respond quickly to infections and initiate immune defenses.

Pathways

TRIF participates in important immune signaling pathways notably the TLR and interferon signaling pathways. In the TLR pathway TRIF interacts with proteins such as MyD88 and TRAF6 to propagate signals from TLR4 critical in detecting Gram-negative bacteria. Through its role in the interferon signaling pathway TRIF helps in the regulation of antiviral responses and is essential for the expression of interferon-stimulated genes that combat viral infections.

TRIF is associated with conditions involving abnormal immune responses such as autoimmune disorders and sepsis. Dysregulation of TRIF-mediated signaling can lead to excessive inflammation or defective immune responses contributing to disease pathogenesis. For example an overactive TRIF pathway may lead to increased susceptibility to sepsis due to augmented inflammatory cytokine production. TRIF also connects with proteins like IRF3 and NF-kB which are often implicated in autoimmune conditions due to their roles in persistent inflammation and immune cell activation.
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Specifications

Form

Liquid

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General info

Function

Involved in innate immunity against invading pathogens. Adapter used by TLR3, TLR4 (through TICAM2) and TLR5 to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis (PubMed : 12471095, PubMed : 12539043, PubMed : 14739303, PubMed : 28747347, PubMed : 35215908). Ligand binding to these receptors results in TRIF recruitment through its TIR domain (PubMed : 12471095, PubMed : 12539043, PubMed : 14739303). Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively (PubMed : 12471095, PubMed : 12539043, PubMed : 14739303). Phosphorylation by TBK1 on the pLxIS motif leads to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent immunity against invading pathogens (PubMed : 25636800). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines (By similarity).

Post-translational modifications

Phosphorylated by TBK1 (PubMed:14530355, PubMed:25636800). Following activation, phosphorylated by TBK1 at Ser-210 in the pLxIS motif (PubMed:25636800). The phosphorylated pLxIS motif constitutes an IRF3-binding motif, leading to recruitment of the transcription factor IRF3 to induce type-I interferons and other cytokines (PubMed:25636800, PubMed:27302953).. Polyubiquitinated at Lys-229 by TRIM38 with 'Lys-48'-linked chains, leading to proteasomal degradation (PubMed:23056470). Polyubiquitinated with 'Lys-6'- and 'Lys-33'-linked chains in a TRIM8-dependent manner; ubiquitination disrupts the interaction with TBK1 and subsequent interferon production (PubMed:28747347).. (Microbial infection) Cleaved and degraded by hepatitis A virus (HAV) protein 3CD allowing the virus to disrupt host TLR3 signaling.. (Microbial infection) Cleaved by CVB3 protease 3C allowing the virus to disrupt host TLR3 signaling.. (Microbial infection) Cleaved by Seneca Valley virus protease 3C allowing the virus to disrupt host TLR3 signaling.. (Microbial infection) Cleaved by protease 3C of human enterovirus D68 (EV68) allowing the virus to disrupt host TLR3 signaling.. (Microbial infection) Cleaved by HCV protease NS3/4A, thereby disrupting TLR3 signaling and preventing the establishment of an antiviral state.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Involved in innate immunity against invading pathogens. Adapter used by TLR3, TLR4 (through TICAM2) and TLR5 to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis (PubMed : 12471095, PubMed : 12539043, PubMed : 14739303, PubMed : 28747347, PubMed : 35215908). Ligand binding to these receptors results in TRIF recruitment through its TIR domain (PubMed : 12471095, PubMed : 12539043, PubMed : 14739303). Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively (PubMed : 12471095, PubMed : 12539043, PubMed : 14739303). Phosphorylation by TBK1 on the pLxIS motif leads to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent immunity against invading pathogens (PubMed : 25636800). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines (By similarity).
See full target information TICAM1
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