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AB239570

Recombinant Human TRIM5 alpha protein (Tagged)

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Recombinant Human TRIM5 alpha protein (Tagged) is a Human Full Length protein, in the 1 to 493 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

RNF88, TRIM5, Tripartite motif-containing protein 5, RING finger protein 88, RING-type E3 ubiquitin transferase TRIM5

1 Images
SDS-PAGE - Recombinant Human TRIM5 alpha protein (Tagged) (AB239570)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TRIM5 alpha protein (Tagged) (AB239570)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab239570.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag N-Terminus B2M tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9C035

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Complementary Products

Primary Antibodies

AB68474

Anti-eIF4B antibody [EP2299Y]

RabMAb

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] (AB68474)

  • 5
  • 1
Primary Antibodies

AB235448

Anti-Acid phosphatase antibody [EPR21787]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21787] (AB235448)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASGILVNVKEEVTCPICLELLTQPLSLDCGHSFCQACLTANHKKSMLDKGESSCPVCRISYQPENIRPNRHVANIVEKLREVKLSPEGQKVDHCARHGEKLLLFCQEDGKVICWLCERSQEHRGHHTFLTEEVAREYQVKLQAALEMLRQKQQEAEELEADIREEKASWKTQIQYDKTNVLADFEQLRDILDWEESNELQNLEKEEEDILKSLTNSETEMVQQTQSLRELISDLEHRLQGSVMELLQGVDGVIKRTENVTLKKPETFPKNQRRVFRAPDLKGMLEVFRELTDVRRYWVDVTVAPNNISCAVISEDKRQVSSPKPQIIYGARGTRYQTFVNFNYCTGILGSQSITSGKHYWEVDVSKKTAWILGVCAGFQPDAMCNIEKNENYQPKYGYWVIGLEEGVKCSAFQDSSFHTPSVPFIVPLSVIICPDRVGVFLDYEACTVSFFNITNHGFLIYKFSHCSFSQPVFPYLNPRKCGVPMTLCSPSS","proteinLength":"Full Length","predictedMolecularWeight":"70.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":493,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9C035","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"B2M","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM5 alpha also known as Tripartite Motif Containing 5 Alpha is a protein that acts as a restriction factor against retroviruses. It has a mass of approximately 56 kDa. This protein expresses in various tissues especially in the lymphoid tissues and is part of the TRIM family of proteins. TRIM5 alpha contains a RING finger B-box type 2 and a coiled-coil domain which enable it to interact with other proteins and bind to virus capsids.
Biological function summary

TRIM5 alpha functions as an innate immune factor that interferes with early stages of retroviral infections in host cells. It does not function alone; it is part of a complex including other TRIM proteins that work together to recognize and block the reverse transcription of viral RNA into DNA. This action prevents viral replication at very early stages providing antiviral defenses even before adaptive immunity kicks in.

Pathways

TRIM5 alpha plays a significant role in the innate immune pathway specifically influencing the recognition and response to retroviral infections. It interacts with proteins like cyclophilin A to enhance its ability to restrict retroviruses like HIV-1. TRIM5 alpha activity also connects with the ubiquitin-proteasome system facilitating the degradation of viral components that enter host cells reducing the potential for infection.

TRIM5 alpha has a strong association with HIV/AIDS due to its role in restricting HIV-1 in certain primates although it does not effectively restrict all strains in humans. Mutations or varying sequence polymorphisms in TRIM5 alpha can influence susceptibility or resistance to HIV infection. It also relates to other retroviral diseases through its interaction with capsid proteins of diverse retroviruses highlighting its broad antiviral role across different species.
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Specifications

Form

Liquid

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General info

Function

Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV) (PubMed : 17156811). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2 (PubMed : 25127057). Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction (PubMed : 25127057).

Sequence similarities

Belongs to the TRIM/RBCC family.

Post-translational modifications

Degraded in a proteasome-independent fashion in the absence of viral infection but in a proteasome-dependent fashion following exposure to restriction sensitive virus.. Autoubiquitinated in a RING finger- and UBE2D2-dependent manner. Monoubiquitinated by TRIM21. Deubiquitinated by Yersinia YopJ. Ubiquitination may not lead to proteasomal degradation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV) (PubMed : 17156811). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2 (PubMed : 25127057). Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction (PubMed : 25127057).
See full target information TRIM5
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