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AB217836

Recombinant human TRIO protein (Active)

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Recombinant human TRIO protein (Active) is a Human Fragment protein, in the 2627 to 3097 aa range, expressed in Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Triple functional domain protein, PTPRF-interacting protein, TRIO

4 Images
Functional Studies - Recombinant human TRIO protein (Active) (AB217836)
  • FuncS

Supplier Data

Functional Studies - Recombinant human TRIO protein (Active) (AB217836)

The specific activity of TRIO (ab217836) was determined to be 1.6 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human TRIO protein (Active) (AB217836)
  • FuncS

Supplier Data

Functional Studies - Recombinant human TRIO protein (Active) (AB217836)

Kinase activity plot. The specific activity was determined to be 1.6 nmol/min/mg.

SDS-PAGE - Recombinant human TRIO protein (Active) (AB217836)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TRIO protein (Active) (AB217836)

SDS PAGE analysis of ab217836

SDS-PAGE - Recombinant human TRIO protein (Active) (AB217836)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human TRIO protein (Active) (AB217836)

The purity of ab217836 was determined to be >80% by densitometry. MW ~82 kDa.

Key facts

Purity

>80% SDS-PAGE

Expression system

Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab217836 was determined to be 1.6 nmol/min/mg.

Accession

O75962

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

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  • 0
Primary Antibodies

AB194365

Anti-TRIO antibody

Western blot - Anti-TRIO antibody (AB194365)

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Cell Lines & Lysates

AB267298

Human TRIO knockout HEK-293T cell line

Sanger Sequencing - Human TRIO knockout HEK-293T cell line (AB267298)

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Cell Lines & Lysates

AB259210

Human TRIO knockout HEK-293T cell lysate

Sanger Sequencing - Human TRIO knockout HEK-293T cell lysate (AB259210)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KKSTSWHTALRLRKKSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGETVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCIAVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKRAVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDCVVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDAVQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEETCLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGVLDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRV","proteinLength":"Fragment","predictedMolecularWeight":"82 kDa","actualMolecularWeight":null,"aminoAcidEnd":3097,"aminoAcidStart":2627,"nature":"Recombinant","expressionSystem":"Sf9 cells","accessionNumber":"O75962","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIO also known as Triple Functional Domain Protein or Trio Rho guanine nucleotide exchange factor acts mechanically as a guanine nucleotide exchange factor. It facilitates the activation of the Rho family of small GTPases specifically Rac1 and RhoG. TRIO weighs approximately 349 kDa and is widely expressed in different tissues especially in the central nervous system. This protein modulates signal transduction associated with cytoskeletal dynamics cell migration and neuronal development.
Biological function summary

TRIO influences cellular processes like actin cytoskeleton organization and axonal outgrowth. It serves as part of multi-protein complexes that guide the intracellular signaling cascades. Particularly TRIO plays a central role in developmental pathways by promoting neuronal growth and differentiation through Rac1 activation. Its function is important for establishing the architecture of the nervous system.

Pathways

TRIO participates in key signal transduction pathways such as the Rho GTPase cycle and the axon guidance pathway. It acts in concert with other proteins like Rac1 and RhoG to regulate cell adhesion and neuronal pathfinding. Through these pathways TRIO contributes to the polarization and directional movement of cells which is necessary for proper tissue and organ development.

TRIO connects to several neurological conditions including intellectual disability and schizophrenia. Mutations in the TRIO gene as well as dysregulation of its related proteins can disrupt normal brain development and function. Particularly alterations in Rac1 and RhoG signaling have been implicated in these disorders linking TRIO to their pathogenesis and potential therapeutic targets.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Guanine nucleotide exchange factor (GEF) for RHOA and RAC1 GTPases (PubMed : 22155786, PubMed : 27418539, PubMed : 8643598). Involved in coordinating actin remodeling, which is necessary for cell migration and growth (PubMed : 10341202, PubMed : 22155786). Plays a key role in the regulation of neurite outgrowth and lamellipodia formation (PubMed : 32109419). In developing hippocampal neurons, limits dendrite formation, without affecting the establishment of axon polarity. Once dendrites are formed, involved in the control of synaptic function by regulating the endocytosis of AMPA-selective glutamate receptors (AMPARs) at CA1 excitatory synapses (By similarity). May act as a regulator of adipogenesis (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Post-translational modifications

Phosphorylated on serine residue(s).

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Product protocols

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Target data

Guanine nucleotide exchange factor (GEF) for RHOA and RAC1 GTPases (PubMed : 22155786, PubMed : 27418539, PubMed : 8643598). Involved in coordinating actin remodeling, which is necessary for cell migration and growth (PubMed : 10341202, PubMed : 22155786). Plays a key role in the regulation of neurite outgrowth and lamellipodia formation (PubMed : 32109419). In developing hippocampal neurons, limits dendrite formation, without affecting the establishment of axon polarity. Once dendrites are formed, involved in the control of synaptic function by regulating the endocytosis of AMPA-selective glutamate receptors (AMPARs) at CA1 excitatory synapses (By similarity). May act as a regulator of adipogenesis (By similarity).
See full target information TRIO
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