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AB99214

Recombinant Human Tropomyosin 1 (alpha) protein

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Recombinant Human Tropomyosin 1 (alpha) protein is a Human Full Length protein, in the 1 to 284 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C15orf13, TMSA, TPM1, Tropomyosin alpha-1 chain, Alpha-tropomyosin, Tropomyosin-1

1 Images
SDS-PAGE - Recombinant Human Tropomyosin 1 (alpha) protein (AB99214)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human Tropomyosin 1 (alpha) protein (AB99214)

15% SDS-PAGE analysis of 3μg ab99214.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P09493

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGQVRQLEEQLRIMDQTLKALMAAEDKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSM","proteinLength":"Full Length","predictedMolecularWeight":"35 kDa","actualMolecularWeight":null,"aminoAcidEnd":284,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09493","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tropomyosin 1 (alpha) also known as alpha-tropomyosin is a protein widely expressed in skeletal and cardiac muscle tissues. This protein plays a central role in muscle contraction by binding to actin filaments. Its precise molecular weight is approximately 32.7 kDa. Alpha-tropomyosin stabilizes actin filaments and regulates the interaction of actin with myosin which is essential for muscular contraction.
Biological function summary

Alpha-tropomyosin ensures the regulation of actin-myosin interactions during muscle contraction. It forms part of the troponin-tropomyosin complex which includes other key proteins such as troponin I T and C. This complex undergoes conformational changes in response to calcium ion concentrations allowing muscle contraction to occur. Alterations in this complex can significantly affect muscle function highlighting the central role of alpha-tropomyosin.

Pathways

Alpha-tropomyosin operates within the actin cytoskeleton regulatory pathway and the calcium signaling pathway. It is closely related to the troponin complex proteins facilitating muscle contraction in these pathways by controlling actin and myosin interactions. Calcium ions bind to troponin C causing structural changes that shift tropomyosin on the actin filament therefore enabling the binding of myosin heads to actin.

Mutations or dysregulation of alpha-tropomyosin have been linked to conditions like hypertrophic cardiomyopathy and nemaline myopathy. These disorders often result from altered interactions within the troponin-tropomyosin complex and other proteins like myosin heavy chain. Defective regulation of these interactions impairs muscle contraction leading to the clinical manifestations seen in these diseases.
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Specifications

Form

Liquid

Additional notes

ab99214 is purified using conventional chromatography techniques.

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General info

Function

Binds to actin filaments in muscle and non-muscle cells (PubMed : 23170982). Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction (PubMed : 23170982). Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

Sequence similarities

Belongs to the tropomyosin family.

Post-translational modifications

Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Binds to actin filaments in muscle and non-muscle cells (PubMed : 23170982). Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction (PubMed : 23170982). Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
See full target information TPM1
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