Recombinant Human TRP2/DCT protein is a Human Full Length protein, in the 1 to 519 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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- In vitro cellular & developmental biology. Animal 52:806-112016The effects of IGF1 on the melanogenesis in alpaca melanocytes in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuaipeng Hu et. al.PubMed 27173613
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M S P L W W G F L L S C L G C K I L P G A Q G Q F P R V C M T V D S L V N K E C C P R L G A E S A N V C G S Q Q G R G Q C T E V R A D T R P W S G P Y I L R N Q D D R E L W P R K F F H R T C K C T G N F A G Y N C G D C K F G W T G P N C E R K K P P V I R Q N I H S L S P Q E R E Q F L G A L D L A K K R V H P D Y V I T T Q H W L G L L G P N G T Q P Q F A N C S V Y D F F V W L H Y Y S V R D T L L G P G R P Y R A I D F S H Q G P A F V T W H R Y H L L C L E R D L Q R L I G N E S F A L P Y W N F A T G R N E C D V C T D Q L F G A A R P D D P T L I S R N S R F S S W E T V C D S L D D Y N H L V T L C N G T Y E G L L R R N Q M G R N S M K L P T L K D I R D C L S L Q K F D N P P F F Q N S T F S F R N A L E G F D K A D G T L D S Q V M S L H N L V H S F L N G T N A L P H S A A N D P I F V V L H S F T D A I F D E W M K R F N P P A D A W P Q E L A P I G H N R M Y N M V P F F P P V T N E E L F L T S D Q L G Y S Y A I D L P V S V E E T P G W P T T L L V V M G T L V A L V G L F V L L A F L Q Y R R L R K G Y T P L M E T H L S S K R Y T E E A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Plays a role in melanin biosynthesis (PubMed:33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).
Alternative names
TYRP2, DCT, L-dopachrome tautomerase, DT, L-dopachrome Delta-isomerase, Tyrosinase-related protein 2, TRP-2, TRP2
Recommended products
Recombinant Human TRP2/DCT protein is a Human Full Length protein, in the 1 to 519 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P40126
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M S P L W W G F L L S C L G C K I L P G A Q G Q F P R V C M T V D S L V N K E C C P R L G A E S A N V C G S Q Q G R G Q C T E V R A D T R P W S G P Y I L R N Q D D R E L W P R K F F H R T C K C T G N F A G Y N C G D C K F G W T G P N C E R K K P P V I R Q N I H S L S P Q E R E Q F L G A L D L A K K R V H P D Y V I T T Q H W L G L L G P N G T Q P Q F A N C S V Y D F F V W L H Y Y S V R D T L L G P G R P Y R A I D F S H Q G P A F V T W H R Y H L L C L E R D L Q R L I G N E S F A L P Y W N F A T G R N E C D V C T D Q L F G A A R P D D P T L I S R N S R F S S W E T V C D S L D D Y N H L V T L C N G T Y E G L L R R N Q M G R N S M K L P T L K D I R D C L S L Q K F D N P P F F Q N S T F S F R N A L E G F D K A D G T L D S Q V M S L H N L V H S F L N G T N A L P H S A A N D P I F V V L H S F T D A I F D E W M K R F N P P A D A W P Q E L A P I G H N R M Y N M V P F F P P V T N E E L F L T S D Q L G Y S Y A I D L P V S V E E T P G W P T T L L V V M G T L V A L V G L F V L L A F L Q Y R R L R K G Y T P L M E T H L S S K R Y T E E A
- Accession
- P40126
- Protein length
- Full Length
- Amino acids
- 1 to 519
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Plays a role in melanin biosynthesis (PubMed:33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).
- Sequence similarities
Belongs to the tyrosinase family.
- Post-translational modifications
Glycosylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Protein concentration is above or equal to 0.05 mg/ml.Previously labelled as TRP2
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRP2 also known as DCT or dopachrome tautomerase is a protein involved in melanin synthesis and is part of the tyrosinase-related protein family. It has a molecular weight of approximately 60 kDa. TRP2/DCT is mainly expressed in melanocytes which are cells responsible for pigment production. The protein functions in the melanin biosynthetic pathway converting dopachrome into DHICA contributing to the eumelanin formation which impacts skin and hair color.
- Biological function summary
TRP2/DCT participates in pigmentation and photoprotection processes. It functions in tandem with other enzymes like tyrosinase and TRP1 to form a multiprotein complex important for melanin synthesis. The coordination of these proteins ensures the effective production and regulation of melanin which absorbs ultraviolet radiation and reduces DNA damage in skin cells.
- Pathways
TRP2/DCT plays a role in the melanin biosynthetic pathway and influences the Wnt signaling pathway. The melanin pathway involves enzymes such as tyrosinase which initiates the conversion of tyrosine to L-DOPA and eventually to melanin pigments. The Wnt signaling pathway critical for cellular processes like proliferation and differentiation interacts with melanogenesis through proteins like beta-catenin influencing TRP2/DCT expression and activity.
- Associated diseases and disorders
TRP2/DCT has significant associations with melanoma and vitiligo. In melanoma altered expression of TRP2/DCT can impact tumor cell proliferation and survival often interacting with proteins like MITF an important regulator of melanocyte development. In vitiligo changes in TRP2/DCT expression can disrupt melanin production leading to depigmented patches on the skin. Understanding these interactions and expressions aids in potential therapeutic approaches for these skin conditions.
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1 product image
SDS-PAGE - Recombinant Human TRP2/DCT protein (ab158267)
ab158267 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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