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AB116176

Recombinant Human TRPT1 protein

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Recombinant Human TRPT1 protein is a Human Full Length protein, in the 1 to 253 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

tRNA 2'-phosphotransferase 1, TRPT1

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SDS-PAGE - Recombinant Human TRPT1 protein (AB116176)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human TRPT1 protein (AB116176)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q86TN4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMNFSGGGRQEAAGSRGRRAPRPREQDRDVQLSKALSYALRHGALKLGLPMGADGFVPLGTLLQLPQFRGFSAEDVQRVVDTNRKQRFALQLGDPSTGLLIRANQGHSLQVPKLELMPLETPQALPPMLVHGTFWKHWPSILLKGLSCQGRTHIHLAPGLPGDPGIISGMRSHCEIAVFIDGPLALADGIPFFRSANGVILTPGNTDGFLLPKYFKEALQLRPTRKPLSLAGDEETECQSSPKHSSRERRRIQQ","proteinLength":"Full Length","predictedMolecularWeight":"30.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":253,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q86TN4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRPT1 also known as TRNA phosphotransferase 1 is an enzyme responsible for the transfer of phosphate groups from tRNA molecules. It carries a molecular mass of around 42 kDa and is widely expressed in human tissues with notable levels in the liver kidney and brain. The enzyme facilitates the transfer of a 2'-phosphate group at the tRNA splicing junction which is an essential step in tRNA splicing and maturation.
Biological function summary

TRPT1 plays a significant role in protein synthesis by ensuring the accurate processing of tRNA molecules. This enzyme is part of the tRNA splicing complex and works closely in conjunction with other components to complete the tRNA maturation. Its activity is important for maintaining the integrity and function of tRNA which is an important player in the translation of genetic information.

Pathways

TRPT1 contributes substantially to the tRNA processing pathway and RNA metabolism. It is involved in pathways related to RNA splicing cooperating with proteins like CLP1 to ensure efficient and error-free splicing. These interactions are critical for maintaining efficient protein synthesis and consequently proper cellular function.

TRPT1's activity links to several conditions one being neurological disorders. Disrupted tRNA processing can lead to dysfunctional protein synthesis which might contribute to neurodegenerative diseases. Furthermore connection between TRPT1 and the protein PNKP implicates its role in disorders related to DNA repair mechanisms such as microcephaly and other neurological impairments.
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Specifications

Form

Liquid

Additional notes

ab116176 was purified using conventional chromatography.

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General info

Function

Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.

Sequence similarities

Belongs to the KptA/TPT1 family.

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Product protocols

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Target data

Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.
See full target information TRPT1
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