Recombinant Human TRPT1 protein is a Human Full Length protein, in the 1 to 253 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M N F S G G G R Q E A A G S R G R R A P R P R E Q D R D V Q L S K A L S Y A L R H G A L K L G L P M G A D G F V P L G T L L Q L P Q F R G F S A E D V Q R V V D T N R K Q R F A L Q L G D P S T G L L I R A N Q G H S L Q V P K L E L M P L E T P Q A L P P M L V H G T F W K H W P S I L L K G L S C Q G R T H I H L A P G L P G D P G I I S G M R S H C E I A V F I D G P L A L A D G I P F F R S A N G V I L T P G N T D G F L L P K Y F K E A L Q L R P T R K P L S L A G D E E T E C Q S S P K H S S R E R R R I Q Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.
Alternative names
tRNA 2'-phosphotransferase 1, TRPT1
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Recombinant Human TRPT1 protein is a Human Full Length protein, in the 1 to 253 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q86TN4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M N F S G G G R Q E A A G S R G R R A P R P R E Q D R D V Q L S K A L S Y A L R H G A L K L G L P M G A D G F V P L G T L L Q L P Q F R G F S A E D V Q R V V D T N R K Q R F A L Q L G D P S T G L L I R A N Q G H S L Q V P K L E L M P L E T P Q A L P P M L V H G T F W K H W P S I L L K G L S C Q G R T H I H L A P G L P G D P G I I S G M R S H C E I A V F I D G P L A L A D G I P F F R S A N G V I L T P G N T D G F L L P K Y F K E A L Q L R P T R K P L S L A G D E E T E C Q S S P K H S S R E R R R I Q Q
- Accession
- Q86TN4
- Protein length
- Full Length
- Predicted molecular weight
- 30.3 kDa
- Amino acids
- 1 to 253
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab116176 was purified using conventional chromatography.
General info
- Function
Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.
- Sequence similarities
Belongs to the KptA/TPT1 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRPT1 also known as TRNA phosphotransferase 1 is an enzyme responsible for the transfer of phosphate groups from tRNA molecules. It carries a molecular mass of around 42 kDa and is widely expressed in human tissues with notable levels in the liver kidney and brain. The enzyme facilitates the transfer of a 2'-phosphate group at the tRNA splicing junction which is an essential step in tRNA splicing and maturation.
- Biological function summary
TRPT1 plays a significant role in protein synthesis by ensuring the accurate processing of tRNA molecules. This enzyme is part of the tRNA splicing complex and works closely in conjunction with other components to complete the tRNA maturation. Its activity is important for maintaining the integrity and function of tRNA which is an important player in the translation of genetic information.
- Pathways
TRPT1 contributes substantially to the tRNA processing pathway and RNA metabolism. It is involved in pathways related to RNA splicing cooperating with proteins like CLP1 to ensure efficient and error-free splicing. These interactions are critical for maintaining efficient protein synthesis and consequently proper cellular function.
- Associated diseases and disorders
TRPT1's activity links to several conditions one being neurological disorders. Disrupted tRNA processing can lead to dysfunctional protein synthesis which might contribute to neurodegenerative diseases. Furthermore connection between TRPT1 and the protein PNKP implicates its role in disorders related to DNA repair mechanisms such as microcephaly and other neurological impairments.
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1 product image
SDS-PAGE - Recombinant Human TRPT1 protein (ab116176)
3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
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