Recombinant Human Tryptophanyl tRNA synthetase/WRS protein is a Human Full Length protein, in the 1 to 471 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M P N S E P A S L L E L F N S I A T Q G E L V R S L K A G N A S K D E I D S A V K M L V S L K M S Y K A A A G E D Y K A D C P P G N P A P T S N H G P D A T E A E E D F V D P W T V Q T S S A K G I D Y D K L I V R F G S S K I D K E L I N R I E R A T G Q R P H H F L R R G I F F S H R D M N Q V L D A Y E N K K P F Y L Y T G R G P S S E A M H V G H L I P F I F T K W L Q D V F N V P L V I Q M T D D E K Y L W K D L T L D Q A Y S Y A V E N A K D I I A C G F D I N K T F I F S D L D Y M G M S S G F Y K N V V K I Q K H V T F N Q V K G I F G F T D S D C I G K I S F P A I Q A A P S F S N S F P Q I F R D R T D I Q C L I P C A I D Q D P Y F R M T R D V A P R I G Y P K P A L L H S T F F P A L Q G A Q T K M S A S D P N S S I F L T D T A K Q I K T K V N K H A F S G G R D T I E E H R Q F G G N C D V D V S F M Y L T F F L E D D D K L E Q I R K D Y T S G A M L T G E L K K A L I E V L Q P L I A E H Q A R R K E V T D E I V K E F M T P R K L S F D F Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp). Isoform 1. Has no angiostatic activity. T2-TrpRS. Possesses an angiostatic activity but has no aminoacylation activity (PubMed:11773625, PubMed:11773626, PubMed:14630953). Inhibits fluid shear stress-activated responses of endothelial cells (PubMed:14630953). Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (PubMed:14630953). Isoform 2. Has an angiostatic activity.
Alternative names
IFI53, WARS, WRS, WARS1, Interferon-induced protein 53, Tryptophanyl-tRNA synthetase, IFP53, TrpRS, hWRS
Recommended products
Recombinant Human Tryptophanyl tRNA synthetase/WRS protein is a Human Full Length protein, in the 1 to 471 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P23381-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M P N S E P A S L L E L F N S I A T Q G E L V R S L K A G N A S K D E I D S A V K M L V S L K M S Y K A A A G E D Y K A D C P P G N P A P T S N H G P D A T E A E E D F V D P W T V Q T S S A K G I D Y D K L I V R F G S S K I D K E L I N R I E R A T G Q R P H H F L R R G I F F S H R D M N Q V L D A Y E N K K P F Y L Y T G R G P S S E A M H V G H L I P F I F T K W L Q D V F N V P L V I Q M T D D E K Y L W K D L T L D Q A Y S Y A V E N A K D I I A C G F D I N K T F I F S D L D Y M G M S S G F Y K N V V K I Q K H V T F N Q V K G I F G F T D S D C I G K I S F P A I Q A A P S F S N S F P Q I F R D R T D I Q C L I P C A I D Q D P Y F R M T R D V A P R I G Y P K P A L L H S T F F P A L Q G A Q T K M S A S D P N S S I F L T D T A K Q I K T K V N K H A F S G G R D T I E E H R Q F G G N C D V D V S F M Y L T F F L E D D D K L E Q I R K D Y T S G A M L T G E L K K A L I E V L Q P L I A E H Q A R R K E V T D E I V K E F M T P R K L S F D F Q
- Accession
- P23381
- Protein length
- Full Length
- Predicted molecular weight
- 55.3 kDa
- Amino acids
- 1 to 471
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab99263 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp).
- Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
- Post-translational modifications
Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as Tryptophanyl tRNA synthetase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tryptophanyl tRNA synthetase also known as WRS or TrpRS is an enzyme responsible for attaching tryptophan to its corresponding tRNA during protein synthesis. This enzyme is approximately 53 kDa in mass. It ensures the accurate translation of the genetic code in mRNA into proteins by catalyzing the esterification of tryptophan to the tRNA^Trp. WRS is mostly found in the cytoplasm of cells across various tissues contributing to its role in protein biosynthesis.
- Biological function summary
WRS is involved in the important process of protein translation ensuring fidelity in the genetic code transfer from RNA to protein. As part of the aminoacyl-tRNA synthetases family WRS helps in maintaining the overall function and stability of the translational apparatus. It is not typically known to be part of any larger protein complex. In addition to its core function in protein synthesis WRS possesses non-canonical roles related to immune response regulation and angiogenesis.
- Pathways
WRS plays key roles in the aminoacyl-tRNA biosynthesis and mTOR signaling pathways. These pathways are essential for regulating protein synthesis and cell growth. In the mTOR pathway WRS's function relates to its interaction with proteins involved in cell growth and proliferation. Its roles within these pathways highlight its influence on cellular homeostasis and protein regulation interacting closely with other synthetases and transcription factors.
- Associated diseases and disorders
WRS has associations with certain cancers and autoimmune disorders. Abnormal expression of WRS is observed in various cancer types reflecting its contribution to dysregulated protein synthesis and cell growth. Additionally WRS has been linked to autoimmune diseases like dermatomyositis where its immune-regulatory roles become compromised. In these conditions WRS's interactions with proteins such as interferons illustrate its importance in the pathophysiology of inflammation and immune response.
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1 product image
SDS-PAGE - Recombinant Human Tryptophanyl tRNA synthetase/WRS protein (ab99263)
15% SDS-PAGE showing ab99263 (3µg).
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