Recombinant Human Tspan-8 protein (Fc Chimera) is a Human Fragment protein, in the 110 to 205 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
K S K S D R I V N E T L Y E N T K L L S A T G E S E K Q F Q E A I I V F Q E E F K C C G L V N G A A D W G N N F Q H Y P E L C A C L D K Q R P C Q S Y N G K Q V Y K E T C I S F I K D F L A K N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed:27180357, PubMed:36078095). Participates thereby in diverse biological functions such as cell signal transduction, migration and protein trafficking (PubMed:25761241). Promotes ADAM17-mediated TNF-alpha processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs) (PubMed:36078095). Forms a complex with RICTOR and integrin alpha3/ITGA3 to mediate mTORC2 activation and AKT1 phosphorylation leading to cell migration (PubMed:25761241). Reduces apoptosis and autophagy induced by high glucose levels through forming a complex with mTOR and RICTOR (PubMed:35904232). Contributes to the maintenance of intestinal epithelial barrier and plays a role in the regulation of intestine inflammation by switching interferon gamma receptor 1/IFNGR1 from clathrin-dependent to lipid raft-dependent endocytosis route to limit STAT1 activation magnitude and duration (PubMed:37204469). Acts as a modulator of the endothelin axis by associating with endothelin converting enzyme ECE1 and regulating its activity of conversion of the endothelin-1 precursor to endothelin (PubMed:37835445).
Alternative names
TM4SF3, TSPAN8, Tetraspanin-8, Tspan-8, Transmembrane 4 superfamily member 3, Tumor-associated antigen CO-029
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Recombinant Human Tspan-8 protein (Fc Chimera) is a Human Fragment protein, in the 110 to 205 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P19075-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- K S K S D R I V N E T L Y E N T K L L S A T G E S E K Q F Q E A I I V F Q E E F K C C G L V N G A A D W G N N F Q H Y P E L C A C L D K Q R P C Q S Y N G K Q V Y K E T C I S F I K D F L A K N
- Accession
- P19075
- Protein length
- Fragment
- Predicted molecular weight
- 37.6 kDa
- Amino acids
- 110 to 205
- Nature
- Recombinant
- Tags
- Fc tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed:27180357, PubMed:36078095). Participates thereby in diverse biological functions such as cell signal transduction, migration and protein trafficking (PubMed:25761241). Promotes ADAM17-mediated TNF-alpha processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs) (PubMed:36078095). Forms a complex with RICTOR and integrin alpha3/ITGA3 to mediate mTORC2 activation and AKT1 phosphorylation leading to cell migration (PubMed:25761241). Reduces apoptosis and autophagy induced by high glucose levels through forming a complex with mTOR and RICTOR (PubMed:35904232). Contributes to the maintenance of intestinal epithelial barrier and plays a role in the regulation of intestine inflammation by switching interferon gamma receptor 1/IFNGR1 from clathrin-dependent to lipid raft-dependent endocytosis route to limit STAT1 activation magnitude and duration (PubMed:37204469). Acts as a modulator of the endothelin axis by associating with endothelin converting enzyme ECE1 and regulating its activity of conversion of the endothelin-1 precursor to endothelin (PubMed:37835445).
- Sequence similarities
Belongs to the tetraspanin (TM4SF) family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tspan-8 also known as Tetraspanin-8 is a protein with a mass of about 27 kDa. This protein belongs to the tetraspanin family characterized by four transmembrane domains. Tspan-8 is expressed in various tissues including the gastrointestinal tract kidney and lung with notable expression in human tumors. It localizes to membranes where it participates in organizing specific microdomains essential for cellular activities like adhesion and migration.
- Biological function summary
Tspan-8 impacts several cellular functions such as motility and invasion. It often acts as part of a complex interacting with integrins and other tetraspanins. Through these interactions Tspan-8 facilitates communication between the extracellular matrix and the cytoskeleton. This interaction affects processes like cellular attachment and extracellular matrix remodeling contributing to its functions in normal physiological and pathological conditions.
- Pathways
Numerous studies tie Tspan-8 to significant biological pathways particularly those involved in signal transduction and cellular movement. It connects closely with the integrin signaling pathway influencing cellular responses to external signals. In this context Tspan-8 interacts with proteins such as integrin αvβ6 contributing to diverse cellular outcomes. Additionally its role within the epithelial-to-mesenchymal transition (EMT) pathway is relevant for its function in cell plasticity and migration.
- Associated diseases and disorders
Tspan-8 links to cancer and inflammatory conditions. Its overexpression associates with tumor progression and metastasis particularly in cancers like colorectal and pancreatic cancer. The protein's interactions with integrin partners notably integrin αvβ6 could enhance cancer cell invasiveness. Moreover inflammation-related diseases might involve Tspan-8 through its influence on inflammatory cell trafficking and invasion contributing to disease pathology. Understanding these interactions can provide insights into novel therapeutic targets.
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SDS-PAGE - Recombinant Human Tspan-8 protein (Fc Chimera) (ab276679)
SDS-PAGE analysis of ab276679
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