JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB101710

Recombinant human TSSK1 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human TSSK1 protein is a Human Full Length protein, in the 1 to 367 aa range, expressed in Baculovirus infected insect cells, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

SPOGA1, SPOGA4, STK22A, STK22D, TSSK1, FKSG81, TSSK1B, Testis-specific serine/threonine-protein kinase 1, TSK-1, TSK1, TSSK-1, Testis-specific kinase 1, Serine/threonine-protein kinase 22A

2 Images
Functional Studies - Recombinant human TSSK1 protein (AB101710)
  • FuncS

Supplier Data

Functional Studies - Recombinant human TSSK1 protein (AB101710)

The specific activity of TSSK1 (ab101710) was determined to be 60 nmol /min/mg as per activity assay protocol.

SDS-PAGE - Recombinant human TSSK1 protein (AB101710)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TSSK1 protein (AB101710)

SDS PAGE analysis of ab101710

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected insect cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of TSSK1 was determined to be 60 nmol/min/mg.

Accession

Q9BXA7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB195879

Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] - Neuronal Marker

KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (AB195879)

  • 0
  • 0
Primary Antibodies

AB195884

Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (AB195884)

  • 0
  • 0
Primary Antibodies

AB204462

HRP Anti-Smad3 antibody [EP568Y]

RabMAb|Recombinant

Western blot - HRP Anti-Smad3 antibody [EP568Y] (AB204462)

  • 0
  • 0
Primary Antibodies

AB185063

HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control

RabMAb|Recombinant

Western blot - HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (AB185063)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MDDAAVLKRRGYLLGINLGEGSYAKVKSAYSERLKFNVAIKIIDRKKAPADFLEKFLPREIEILAMLNHCSIIKTYEIFETSHGKVYIVMELAVQGDLLELIKTRGALHEDEARKKFHQLSLAIKYCHDLDVVHRDLKCDNLLLDKDFNIKLSDFSFSKRCLRDDSGRMALSKTFCGSPAYAAPEVLQGIPYQPKVYDIWSLGVILYIMVCGSMPYDDSNIKKMLRIQKEHRVNFPRSKHLTGECKDLIYHMLQPDVNRRLHIDEILSHCWMQPKARGSPSVAINKEGESSRGTEPLWTPEPGSDKKSATKLEPEGEAQPQAQPETKPEGTAMQMSRQSEILGFPSKPSTMETEEGPPQQPPETRAQ","proteinLength":"Full Length","predictedMolecularWeight":"71 kDa","actualMolecularWeight":null,"aminoAcidEnd":367,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"Q9BXA7","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSSK1 also known as Testis-Specific Serine Kinase 1 is a protein with a mass of approximately 36 kDa. This kinase is highly specialized and expressed mainly in the testes where it plays a role in male reproductive tissues. As a member of the serine/threonine kinase family TSSK1 transfers phosphate groups to serine residues on target proteins modulating their activity. This action is key for regulating various cellular processes especially in germ cells where it influences signaling pathways important for proper sperm development.
Biological function summary

TSSK1 contributes significantly to spermatogenesis and fertility. It forms part of a complex with other proteins to facilitate its role within the reproductive system. The protein modulates essential cellular processes such as cell division and differentiation during sperm cell maturation. Its activity supports the structural and functional integrity of the developing sperm essential for effective fertilization.

Pathways

TSSK1 activity integrates within signaling cascades related to reproductive functions. It participates in pathways like the protein phosphorylation pathway influencing partners such as TSSK2 and TSSK3 which share overlapping roles in spermatogenesis. Such interactions highlight the focus of TSSK1 in coordinating processes that ensure proper sperm function and associated developmental pathways.

TSSK1 links to conditions affecting male fertility including non-obstructive azoospermia and oligozoospermia. Disruption in TSSK1 function can lead to faulty sperm production contributing to infertility. Through its involvement in these conditions TSSK1 may also interact with proteins like YBX3 which holds roles in RNA regulation during spermatogenesis further underpinning the complex network of processes integral to reproductive health.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-288' of TSKS. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Post-translational modifications

Autophosphorylated.. Ubiquitinated; HSP90 activity negatively regulates ubiquitination and degradation.

style
left-column

Product protocols

style
left-column

Target data

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-288' of TSKS. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.
See full target information TSSK1B
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com