Recombinant Human TSTA3 protein is a Human Full Length protein, in the 1 to 321 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G E P Q G S M R I L V T G G S G L V G K A I Q K V V A D G A G L P G E D W V F V S S K D A D L T D T A Q T R A L F E K V Q P T H V I H L A A M V G G L F R N I K Y N L D F W R K N V H M N D N V L H S A F E V G A R K V V S C L S T C I F P D K T T Y P I D E T M I H N G P P H N S N F G Y S Y A K R M I D V Q N R A Y F Q Q Y G C T F T A V I P T N V F G P H D N F N I E D G H V L P G L I H K V H L A K S S G S A L T V W G T G N P R R Q F I Y S L D L A Q L F I W V L R E Y N E V E P I I L S V G E E D E V S I K E A A E A V V E A M D F H G E V T F D T T K S D G Q F K K T A S N S K L R T Y L P D F R F T P F K Q A V K E T C A W F T D N Y E Q A R K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.
Alternative names
SDR4E1, TSTA3, GFUS, GDP-L-fucose synthase, Protein FX, Red cell NADP(H)-binding protein, Short-chain dehydrogenase/reductase family 4E member 1
Recommended products
Recombinant Human TSTA3 protein is a Human Full Length protein, in the 1 to 321 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q13630-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.29% Sodium chloride, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G E P Q G S M R I L V T G G S G L V G K A I Q K V V A D G A G L P G E D W V F V S S K D A D L T D T A Q T R A L F E K V Q P T H V I H L A A M V G G L F R N I K Y N L D F W R K N V H M N D N V L H S A F E V G A R K V V S C L S T C I F P D K T T Y P I D E T M I H N G P P H N S N F G Y S Y A K R M I D V Q N R A Y F Q Q Y G C T F T A V I P T N V F G P H D N F N I E D G H V L P G L I H K V H L A K S S G S A L T V W G T G N P R R Q F I Y S L D L A Q L F I W V L R E Y N E V E P I I L S V G E E D E V S I K E A A E A V V E A M D F H G E V T F D T T K S D G Q F K K T A S N S K L R T Y L P D F R F T P F K Q A V K E T C A W F T D N Y E Q A R K
- Accession
- Q13630
- Protein length
- Full Length
- Predicted molecular weight
- 38 kDa
- Amino acids
- 1 to 321
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab99245 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.
- Sequence similarities
Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Trans-sialidase-related protein 3 (TSTA3) also known as GDP-L-fucose synthase is an enzyme responsible for converting GDP-4-keto-6-deoxy-D-mannose to GDP-L-fucose an important step in fucose synthesis. TSTA3 has a molecular mass of approximately 36 kDa. This protein expresses widely with significant expression in tissues like the liver which is a central site for metabolic processes.
- Biological function summary
TSTA3 plays a role in fucosylation a fundamental process for the modification of glycoproteins and glycolipids. Fucosylation affects cellular recognition and signaling events. TSTA3 functions independently and as part of no known physical complex but influences several biological activities indirectly through its enzymatic product GDP-L-fucose. This product is critical in multiple cellular functions and structural carbohydrate assemblies.
- Pathways
TSTA3 participates in the GDP-L-fucose biosynthesis pathway an essential route for the production of fucose necessary for fucosylation processes. This pathway ties directly to protein interactions involving glycosyltransferases such as fucosyltransferases which facilitate the transfer of fucose to glycoproteins and glycolipids. TSTA3's contribution to these pathways affects cell adhesion and immune response signaling.
- Associated diseases and disorders
TSTA3 relates mainly to cancers and congenital disorders of glycosylation. Abnormal fucosylation patterns often due to altered TSTA3 activity have been linked to various cancers impacting proteins like E-selectin which is involved in leukocyte adhesion. Additionally congenital disorders of glycosylation sometimes result from deficiencies in enzymes such as TSTA3 leading to disrupted glycan synthesis and presentation connecting to proteins such as glycoproteins with critical physiological roles.
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1 product image
SDS-PAGE - Recombinant Human TSTA3 protein (ab99245)
15% SDS-PAGE showing ab99245 (3μg).
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