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AB271775

Recombinant human TTBK1 protein (GST N-Terminus + His tag C-Terminus)

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Recombinant human TTBK1 protein (GST N-Terminus + His tag C-Terminus) is a Human Fragment protein, in the 1 to 479 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

BDTK, KIAA1855, TTBK1, Tau-tubulin kinase 1, Brain-derived tau kinase

2 Images
Functional Studies - Recombinant human TTBK1 protein (GST N-Terminus + His tag C-Terminus) (AB271775)
  • FuncS

Unknown

Functional Studies - Recombinant human TTBK1 protein (GST N-Terminus + His tag C-Terminus) (AB271775)

Specific activity of ab271775.

SDS-PAGE - Recombinant human TTBK1 protein (GST N-Terminus + His tag C-Terminus) (AB271775)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human TTBK1 protein (GST N-Terminus + His tag C-Terminus) (AB271775)

SDS-PAGE analysis of ab271775.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Assay Conditions: Assay was done in a Kinase buffer using MBP (0.1 mg/ml) as a substrate with 20 μM ATP at 30°C for 45 min. The amount of ATP transferred was calculated using Kinase-Glo reagent.

Accession

Q5TCY1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.06% Glutathione, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MQCLAAALKDETNMSGGGEQADILPANYVVKDRWKVLKKIGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLDFGLARQYTNTTGDVRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIASLDYFTKPDYQLIMSVFENSMKERGIAENEAFDWEKAGTDALLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPILPGRPSEGLGPSPHLVPHPGGPEAEVWEETDVNRNKLRINIGKSPCVEEEQSRGMGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLSTADGRVELPERRSRMD","proteinLength":"Fragment","predictedMolecularWeight":"81 kDa","actualMolecularWeight":null,"aminoAcidEnd":479,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q5TCY1","tags":[{"tag":"GST","terminus":"N-Terminus"},{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau-tubulin kinase 1 (TTBK1) also known as TTBK-1 plays an important role in phosphorylating proteins involved in microtubule dynamics. It has a molecular weight of approximately 150 kDa. TTBK1 is highly expressed in the central nervous system especially in the brain but also occurs in other tissues to a lesser extent. The protein functions as a serine/threonine kinase targeting tau and tubulin proteins amongst others which are essential for maintaining neuronal structure and function.
Biological function summary

This kinase takes part in stabilizing microtubules by phosphorylating tau a protein important for microtubule assembly and stabilization. TTBK1's action helps regulate neuronal cell signaling and maintains proper axonal transport. It does not function in solitude but associates with other proteins as it may form part of larger multi-protein complexes essential for executing its functions. The phosphorylation activity of TTBK1 modulates interactions between microtubules and other cytoskeletal elements in neurons.

Pathways

TTBK1 activities are integral to the tau phosphorylation pathway and the Wnt signaling pathway. In the tau phosphorylation pathway it works alongside other kinases like glycogen synthase kinase 3 beta (GSK3β) contributing to tau modifications affecting microtubule stability. Moreover through Wnt signaling TTBK1 influences pathways responsible for synaptic plasticity and nerve cell communication interacting with proteins like beta-catenin and the Dishevelled protein family. These pathways impact neuron development and functioning.

TTBK1's role is linked significantly to Alzheimer's disease and other neurodegenerative disorders. The over-phosphorylation of tau by TTBK1 contributes to tau aggregation forming neurofibrillary tangles a hallmark of Alzheimer's disease. In this context TTBK1 interacts with tau and partners with GSK3β in pathways that lead to abnormal tau phosphorylation. Mutations or dysregulation of TTBK1 could also contribute to Parkinson's disease emphasizing its involvement in neurodegeneration processes driven by improper tau metabolism and/or interaction with alpha-synuclein.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Serine/threonine kinase which is able to phosphorylate TAU on serine, threonine and tyrosine residues. Induces aggregation of TAU.

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.

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Product protocols

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Target data

Serine/threonine kinase which is able to phosphorylate TAU on serine, threonine and tyrosine residues. Induces aggregation of TAU.
See full target information TTBK1
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