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AB89589

Recombinant human TTK/Mps1 protein

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Recombinant human TTK/Mps1 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

MPS1, MPS1L1, TTK, Dual specificity protein kinase TTK, Phosphotyrosine picked threonine-protein kinase, PYT

5 Images
Functional Studies - Recombinant human TTK/Mps1 protein (AB89589)
  • FuncS

Unknown

Functional Studies - Recombinant human TTK/Mps1 protein (AB89589)

The specific activity of TTK/Mps1 (ab89589) was determined to be 18 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human TTK/Mps1 protein (AB89589)
  • FuncS

Unknown

Functional Studies - Recombinant human TTK/Mps1 protein (AB89589)

Kinase Assay demonstrating specific activity of ab89589.

Western blot - Recombinant human TTK/Mps1 protein (AB89589)
  • WB

Unknown

Western blot - Recombinant human TTK/Mps1 protein (AB89589)

ab11108 recognizes the full length tagged recombinant TTK/Mps1 protein (ab89589) which has an expected molecular weight of 130 kDa.

All lanes:

Western blot - Anti-TTK/Mps1 antibody [N1] - BSA free (<a href='/en-us/products/primary-antibodies/ttk-mps1-antibody-n1-bsa-free-ab11108'>ab11108</a>) at 10 µg/mL

All lanes:

Western blot - Recombinant human TTK/Mps1 protein (ab89589) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 97 kDa

true

Exposure time: 8min

SDS-PAGE - Recombinant human TTK/Mps1 protein (AB89589)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TTK/Mps1 protein (AB89589)

SDS-PAGE showing ab89589 at approximately 130kDa.

SDS-PAGE - Recombinant human TTK/Mps1 protein (AB89589)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human TTK/Mps1 protein (AB89589)

SDS PAGE analysis of ab89589

Key facts

Purity

>75% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The Specific activity of ab89589 was determined to be 19 nmol/min/mg.

Accession

P33981

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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  • 0
Primary Antibodies

AB187520

Anti-TTK/Mps1 antibody

Western blot - Anti-TTK/Mps1 antibody (AB187520)

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  • 1
Primary Antibodies

AB121989

Anti-DNAH8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNAH8 antibody (AB121989)

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Proteins & Peptides

AB152423

Recombinant Human GFPT1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human GFPT1 protein (GST tag N-Terminus) (AB152423)

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Proteins & Peptides

AB116177

Recombinant Human PINK1 protein (denatured) (Tag Free)

SDS-PAGE - Recombinant Human PINK1 protein (denatured) (Tag Free) (AB116177)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab89589 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/ttk-mps1-antibody-n1-bsa-free-ab11108'>ab11108</a>.</p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P33981","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTK also known as Mps1 is a dual-specificity protein kinase with a molecular mass of approximately 97 kDa. It plays a mechanical role in the regulation of the cell cycle by phosphorylating various substrates required for accurate chromosome alignment and segregation during mitosis. TTK/Mps1 is expressed in many tissues but shows the highest levels in rapidly dividing cells such as in the testis and in tumors. The presence of this protein in proliferative tissues suggests its involvement in cell cycle control mechanisms.
Biological function summary

TTK/Mps1 protein is essential for maintaining genomic stability. It functions as part of the mitotic checkpoint complex (MCC) which prevents cells from prematurely advancing from metaphase to anaphase until all chromosomes are correctly aligned on the mitotic spindle. By doing so TTK/Mps1 ensures proper chromosomal attachment and tension at kinetochores reducing the likelihood of aneuploidy. This regulation highlights its critical role in preventing genetic variation during cell division.

Pathways

TTK/Mps1 is an important component of the spindle assembly checkpoint (SAC) pathway which plays an important role in cell cycle regulation. It works closely with other proteins such as BUBR1 BUB3 and MAD2 to halt cell cycle progression until chromosomal alignment is verified. In addition TTK/Mps1 interacts with the aurora kinase A (AURKA) pathway which is instrumental in centrosome maturation and spindle assembly further emphasizing its role in ensuring accurate mitosis.

Aberrations in TTK/Mps1 activity are associated with cancer and aneuploidy. Overexpression or amplification of TTK/Mps1 is frequently observed in various tumors linking it with cancer progression and poor prognosis. Its interaction with other proteins like p53 a well-known tumor suppressor is significant in understanding its role in oncogenesis. Therapeutic targeting of TTK/Mps1 is being explored to exploit its critical function in rapidly proliferating cancer cells aiming to induce mitotic catastrophe and mitigate tumor growth.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments (PubMed : 18243099, PubMed : 28441529, PubMed : 29162720). Phosphorylates MAD1L1 to promote the mitotic spindle assembly checkpoint (PubMed : 18243099, PubMed : 29162720). Phosphorylates CDCA8/Borealin leading to enhanced AURKB activity at the kinetochore (PubMed : 18243099). Phosphorylates SKA3 at 'Ser-34' leading to dissociation of the SKA complex from microtubules and destabilization of microtubule-kinetochore attachments (PubMed : 28441529). Phosphorylates KNL1, KNTC1 and autophosphorylates (PubMed : 28441529). Phosphorylates MCRS1 which enhances recruitment of KIF2A to the minus end of spindle microtubules and promotes chromosome alignment (PubMed : 30785839).

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

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Product protocols

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Target data

Involved in mitotic spindle assembly checkpoint signaling, a process that delays anaphase until chromosomes are bioriented on the spindle, and in the repair of incorrect mitotic kinetochore-spindle microtubule attachments (PubMed : 18243099, PubMed : 28441529, PubMed : 29162720). Phosphorylates MAD1L1 to promote the mitotic spindle assembly checkpoint (PubMed : 18243099, PubMed : 29162720). Phosphorylates CDCA8/Borealin leading to enhanced AURKB activity at the kinetochore (PubMed : 18243099). Phosphorylates SKA3 at 'Ser-34' leading to dissociation of the SKA complex from microtubules and destabilization of microtubule-kinetochore attachments (PubMed : 28441529). Phosphorylates KNL1, KNTC1 and autophosphorylates (PubMed : 28441529). Phosphorylates MCRS1 which enhances recruitment of KIF2A to the minus end of spindle microtubules and promotes chromosome alignment (PubMed : 30785839).
See full target information TTK
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