Recombinant Human TUBA1B protein is a Human Full Length protein, in the 1 to 451 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M R E C I S I H V G Q A G V Q I G N A C W E L Y C L E H G I Q P D G Q M P S D K T I G G G D D S F N T F F S E T G A G K H V P R A V F V D L E P T V I D E V R T G T Y R Q L F H P E Q L I T G K E D A A N N Y A R G H Y T I G K E I I D L V L D R I R K L A D Q C T G L Q G F L V F H S F G G G T G S G F T S L L M E R L S V D Y G K K S K L E F S I Y P A P Q V S T A V V E P Y N S I L T T H T T L E H S D C A F M V D N E A I Y D I C R R N L D I E R P T Y T N L N R L I S Q I V S S I T A S L R F D G A L N V D L T E F Q T N L V P Y P R I H F P L A T Y A P V I S A E K A Y H E Q L S V A E I T N A C F E P A N Q M V K C D P R H G K Y M A C C L L Y R G D V V P K D V N A A I A T I K T K R S I Q F V D W C P T G F K V G I N Y Q P P T V V P G G D L A K V Q R A V C M L S N T T A I A E A W A R L D H K F D L M Y A K R A F V H W Y V G E G M E E G E F S E A R E D M A A L E K D Y E E V G V D S V E G E G E E E G E E Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871).
Alternative names
Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B
Recommended products
Recombinant Human TUBA1B protein is a Human Full Length protein, in the 1 to 451 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P68363-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M R E C I S I H V G Q A G V Q I G N A C W E L Y C L E H G I Q P D G Q M P S D K T I G G G D D S F N T F F S E T G A G K H V P R A V F V D L E P T V I D E V R T G T Y R Q L F H P E Q L I T G K E D A A N N Y A R G H Y T I G K E I I D L V L D R I R K L A D Q C T G L Q G F L V F H S F G G G T G S G F T S L L M E R L S V D Y G K K S K L E F S I Y P A P Q V S T A V V E P Y N S I L T T H T T L E H S D C A F M V D N E A I Y D I C R R N L D I E R P T Y T N L N R L I S Q I V S S I T A S L R F D G A L N V D L T E F Q T N L V P Y P R I H F P L A T Y A P V I S A E K A Y H E Q L S V A E I T N A C F E P A N Q M V K C D P R H G K Y M A C C L L Y R G D V V P K D V N A A I A T I K T K R S I Q F V D W C P T G F K V G I N Y Q P P T V V P G G D L A K V Q R A V C M L S N T T A I A E A W A R L D H K F D L M Y A K R A F V H W Y V G E G M E E G E F S E A R E D M A A L E K D Y E E V G V D S V E G E G E E E G E E Y
- Accession
- P68363
- Protein length
- Full Length
- Amino acids
- 1 to 451
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871).
- Sequence similarities
Belongs to the tubulin family.
- Post-translational modifications
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity).
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TUBA1B also known as tubulin alpha-1B chain is a subunit of microtubules that plays a structural role in the cellular cytoskeleton. It has a molecular mass of about 50 kDa. TUBA1B distributes widely across various tissues with elevated expression in neuronal cells. It characterizes by its involvement in the assembly and stabilization of microtubules which are essential for maintaining cell shape promoting intracellular transport and facilitating chromosome segregation during cell division.
- Biological function summary
TUBA1B contributes significantly to the formation and function of the cytoskeletal microtubule network. It functions as part of the heterodimeric complex pairing with beta-tubulin to form the building blocks of microtubules. These structures are central to diverse cellular activities such as intracellular trafficking axonal transport in neurons and the migration and division of cells. In this implicit role TUBA1B influences the dynamics and stability of microtubule polymers impacting the cell’s ability to respond to mechanical and environmental stimuli.
- Pathways
TUBA1B is integral to the mitotic spindle assembly pathway and neuronal signaling. In the context of these pathways TUBA1B's interactions with proteins like dynein and kinesin facilitate its role in intracellular transport and spindle orientation during cell division. TUBA1B also participates in neuronal development pathways where its association with tau and other microtubule-associated proteins contributes to the formation of neuronal axons and dendrites.
- Associated diseases and disorders
Alterations in TUBA1B have been linked to certain neurodevelopmental disorders and cancers such as lissencephaly and glioblastoma. In lissencephaly mutations in TUBA1B disrupt proper neuronal migration contributing to the abnormal brain development associated with the disorder. In glioblastoma TUBA1B's aberrant expression impacts microtubule dynamics aiding in cancer cell proliferation and resistance to therapies. These pathological conditions often involve interactions with other proteins including those found in the microtubule-associated protein family such as MAP2 and MAPT which further influence disease progression.
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1 product image
SDS-PAGE - Recombinant Human TUBA1B protein (ab160967)
ab160967 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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