Recombinant Human TUFM protein (His tag) is a Human Full Length protein, in the 44 to 452 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A V E A K K T Y V R D K P H V N V G T I G H V D H G K T T L T A A I T K I L A E G G G A K F K K Y E E I D N A P E E R A R G I T I N A A H V E Y S T A A R H Y A H T D C P G H A D Y V K N M I T G T A P L D G C I L V V A A N D G P M P Q T R E H L L L A R Q I G V E H V V V Y V N K A D A V Q D S E M V E L V E L E I R E L L T E F G Y K G E E T P V I V G S A L C A L E G R D P E L G L K S V Q K L L D A V D T Y I P V P A R D L E K P F L L P V E A V Y S V P G R G T V V T G T L E R G I L K K G D E C E L L G H S K N I R T V V T G I E M F H K S L E R A E A G D N L G A L V R G L K R E D L R R G L V M V K P G S I K P H Q K V E A Q V Y I L S K E E G G R H K P F V S H F M P V M F S L T W D M A C R I I L P P E K E L A M P G E D L K F N L I L R Q P M I L E K G Q R F T L R D G N R T I G T G L V T N T L A M T E E E K N I K W G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Also plays a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.
Alternative names
EF-Tu, P43, TUFM
Recommended products
Recombinant Human TUFM protein (His tag) is a Human Full Length protein, in the 44 to 452 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P49411-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A V E A K K T Y V R D K P H V N V G T I G H V D H G K T T L T A A I T K I L A E G G G A K F K K Y E E I D N A P E E R A R G I T I N A A H V E Y S T A A R H Y A H T D C P G H A D Y V K N M I T G T A P L D G C I L V V A A N D G P M P Q T R E H L L L A R Q I G V E H V V V Y V N K A D A V Q D S E M V E L V E L E I R E L L T E F G Y K G E E T P V I V G S A L C A L E G R D P E L G L K S V Q K L L D A V D T Y I P V P A R D L E K P F L L P V E A V Y S V P G R G T V V T G T L E R G I L K K G D E C E L L G H S K N I R T V V T G I E M F H K S L E R A E A G D N L G A L V R G L K R E D L R R G L V M V K P G S I K P H Q K V E A Q V Y I L S K E E G G R H K P F V S H F M P V M F S L T W D M A C R I I L P P E K E L A M P G E D L K F N L I L R Q P M I L E K G Q R F T L R D G N R T I G T G L V T N T L A M T E E E K N I K W G
- Accession
- P49411
- Protein length
- Full Length
- Predicted molecular weight
- 49 kDa
- Amino acids
- 44 to 452
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Also plays a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.
- Sequence similarities
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TUFM also known as elongation factor Tu mitochondrial (EF-Tu) is a protein involved in the protein synthesis process within mitochondria. It has a molecular mass of approximately 50 kDa. TUFM plays an essential role in the translation step of protein synthesis by binding aminoacyl-tRNA and positioning it on the ribosome. Researchers have identified the protein as highly expressed in tissues with high energy demand such as the heart brain and skeletal muscle.
- Biological function summary
TUFM assists in mitochondrial protein synthesis which is necessary for the proper function and maintenance of the respiratory chain complexes. It forms part of the mitochondrial ribosome complex operating alongside other elongation factors like EF-G. TUFM’s activity is essential for the production of proteins encoded by mitochondrial DNA which are they key components of oxidative phosphorylation machinery.
- Pathways
TUFM integrates into the mitochondrial translation pathway which supports the production of proteins critical for the electron transport chain. This pathway is important for ATP production through oxidative phosphorylation. TUFM interacts with other components such as MRPS18 and MRPL3 to mediate the elongation cycle during mitochondrial protein synthesis. These relationships are essential for coordinating mitochondrial function and bioenergetics.
- Associated diseases and disorders
TUFM mutations or disruptions have connections to mitochondrial disorders such as MELAS syndrome (Mitochondrial Encephalomyopathy Lactic Acidosis and Stroke-like episodes) which affects energy production. The protein's dysfunction can also relate to Parkinson's Disease where impaired mitochondrial activity plays a role. TUFM’s interaction with other mitochondrial proteins like NDUFS1 part of complex I of the electron transport chain underlines its potential impact in these disorders.
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1 product image
SDS-PAGE - Recombinant Human TUFM protein (His tag) (ab236184)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab236184.
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