Recombinant Human TWF1/Twinfilin-1 protein is a Human Fragment protein, in the 100 to 350 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M M L Y A A T R A T L K K E F G G G H I K D E V F G T V K E D V S L H G Y K K Y L L S Q S S P A P L T A A E E E L R Q I K I N E V Q T D V G V D T K H Q T L Q G V A F P I S R E A F Q A L E K L N N R Q L N Y V Q L E I D I K N E I I I L A N T T N T E L K D L P K R I P K D S A R Y H F F L Y K H S H E G D Y L E S I V F I Y S M P G Y T C S I R E R M L Y S S C K S R L L E I V E R Q L Q M D V I R K I E I D N G D E L T A D F L Y E E V H P K Q H A H K Q S F A K P K G P A G K R G I R R L I R G P A E T E A T T D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).
Alternative names
PTK9, TWF1, Twinfilin-1, Protein A6, Protein tyrosine kinase 9
Recommended products
Recombinant Human TWF1/Twinfilin-1 protein is a Human Fragment protein, in the 100 to 350 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q12792-2
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M M L Y A A T R A T L K K E F G G G H I K D E V F G T V K E D V S L H G Y K K Y L L S Q S S P A P L T A A E E E L R Q I K I N E V Q T D V G V D T K H Q T L Q G V A F P I S R E A F Q A L E K L N N R Q L N Y V Q L E I D I K N E I I I L A N T T N T E L K D L P K R I P K D S A R Y H F F L Y K H S H E G D Y L E S I V F I Y S M P G Y T C S I R E R M L Y S S C K S R L L E I V E R Q L Q M D V I R K I E I D N G D E L T A D F L Y E E V H P K Q H A H K Q S F A K P K G P A G K R G I R R L I R G P A E T E A T T D
- Accession
- Q12792
- Protein length
- Fragment
- Predicted molecular weight
- 31.5 kDa
- Amino acids
- 100 to 350
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
purified by using conventional chromatography
General info
- Function
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).
- Sequence similarities
Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
- Post-translational modifications
Phosphorylated on serine and threonine residues.
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as TWF1
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TWF1 also known as Twinfilin-1 or A6-related protein is an actin-binding protein with a molecular mass of about 38 kDa. This protein is expressed in various tissues including skeletal muscle heart and brain. TWF1 playing a role in actin dynamics binds to actin monomers and may affect actin filament turnover. It acts by promoting actin depolymerization and inhibiting actin nucleation contributing to the dynamic remodeling of the actin cytoskeleton.
- Biological function summary
TWF1 is important in regulating cell motility morphology and division by influencing actin filament dynamics. TWF1 does not necessarily exist as part of a complex but its activity interacts with components of the cytoskeleton. By modulating actin dynamics TWF1 influences processes such as endocytosis cellular movement and response to external stimuli.
- Pathways
Actin dynamics mediated by TWF1 is integral to the cytoskeleton organization pathway and the endocytic pathway. Within these pathways it interacts indirectly with proteins like cofilin and profilin which also regulate actin cytoskeleton. Its activity helps cells to adapt their shape and movement facilitating diverse cellular functions and signaling processes.
- Associated diseases and disorders
TWF1 has relevance to cancer progression and conditions affecting cell mobility. Abnormal regulation of actin dynamics due to altered TWF1 activity might lead to enhanced cancer cell invasion and metastasis. Additionally TWF1 connects with proteins like ADF/Cofilin regarding cancer-related pathways influencing actin structure remodeling and cell movement in malignant cells.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human TWF1/Twinfilin-1 protein (ab116148)
15% SDS-PAGE showing ab116148 at approximately 31.5kDa (3µg)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com