Recombinant Human Twist protein is a Human Full Length protein, in the 1 to 202 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Publications
Filter by
- The Journal of biological chemistry 294:5082-50932019Protein kinase Cα-mediated phosphorylation of Twist1 at Ser-144 prevents Twist1 ubiquitination and stabilizes it.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoslyn Tedja et. al.PubMed 30733340
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M M Q D V S S S P V S P A D D S L S N S E E E P D R Q Q P P S G K R G G R K R R S S R R S A G G G A G P G G A A G G G V G G G D E P G S P A Q G K R G K K S A G C G G G G G A G G G G G S S S G G G S P Q S Y E E L Q T Q R V M A N V R E R Q R T Q S L N E A F A A L R K I I P T L P S D K L S K I Q T L K L A A R Y I D F L Y Q V L Q S D E L D S K M A S C S Y V A H E R L S Y A F S V W R M E G A W S M S A S H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-BMAL1 heterodimer (By similarity).
Alternative names
BHLHA38, TWIST, TWIST1, Twist-related protein 1, Class A basic helix-loop-helix protein 38, H-twist, bHLHa38
Recommended products
Recombinant Human Twist protein is a Human Full Length protein, in the 1 to 202 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q15672-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M M Q D V S S S P V S P A D D S L S N S E E E P D R Q Q P P S G K R G G R K R R S S R R S A G G G A G P G G A A G G G V G G G D E P G S P A Q G K R G K K S A G C G G G G G A G G G G G S S S G G G S P Q S Y E E L Q T Q R V M A N V R E R Q R T Q S L N E A F A A L R K I I P T L P S D K L S K I Q T L K L A A R Y I D F L Y Q V L Q S D E L D S K M A S C S Y V A H E R L S Y A F S V W R M E G A W S M S A S H
- Accession
- Q15672
- Protein length
- Full Length
- Predicted molecular weight
- 47.85 kDa
- Amino acids
- 1 to 202
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-BMAL1 heterodimer (By similarity).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Twist protein also known as Twist1 is a basic helix-loop-helix (bHLH) transcription factor. It has a molecular mass of approximately 21 kDa. Twist is widely expressed in mesodermal tissues during embryonic development including the somites and limb buds. In adults its expression continues in areas involved in tissue regeneration and wound healing.
- Biological function summary
Twist plays significant roles in embryogenesis and in wound healing processes. It functions as an important regulator of mesenchymal cell differentiation and is part of the bHLH family of transcription factors which often form dimers to bind specific DNA sequences. Twist modulates the transcription of genes involved in cell movement and survival. It acts as an important player in the epithelial-mesenchymal transition (EMT) a process important for development and cellular dynamics.
- Pathways
Twist integrates into multiple biological networks including the Wnt and Notch signaling pathways. It interacts with proteins like β-catenin in the Wnt pathway and Notch receptors impacting cellular proliferation and fate determination. These interactions highlight its role in regulating cellular behavior and influence various processes such as stem cell maintenance and differentiation.
- Associated diseases and disorders
Twist is implicated in cancer and fibrosis. Its overexpression is often observed in aggressive tumors influencing metastasis through its role in EMT. Twist can interact with oncogenic proteins such as MYC in cancer pathways. In fibrosis aberrant Twist activity leads to excessive tissue remodeling and accumulation of connective tissue. Understanding Twist's function and interactions can aid in developing therapeutic strategies targeting these conditions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Twist protein (ab132349)
12.5% SDS-PAGE analysis of ab132349 stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com