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AB99218

Recombinant Human TXNDC4 protein (His tag N-Terminus)

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Recombinant Human TXNDC4 protein (His tag N-Terminus) is a Human Full Length protein, in the 30 to 406 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

KIAA0573, TXNDC4, UNQ532/PRO1075, ERP44, Endoplasmic reticulum resident protein 44, ER protein 44, ERp44, Thioredoxin domain-containing protein 4

1 Images
Western blot - Recombinant Human TXNDC4 protein (His tag N-Terminus) (AB99218)
  • WB

Unknown

Western blot - Recombinant Human TXNDC4 protein (His tag N-Terminus) (AB99218)

15% SDS-PAGE analysis of 3μg ab99218.

All lanes:

Western blot - Recombinant Human TXNDC4 protein (His tag N-Terminus) (ab99218)

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Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9BS26

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWAGSMEITSLDTENIDEILNNADVALVNFYADWCRFSQMLHPIFEEASDVIKEEFPNENQVVFARVDCDQHSDIAQRYRISKYPTLKLFRNGMMMKREYRGQRSVKALADYIRQQKSDPIQEIRDLAEITTLDRSKRNIIGYFEQKDSDNYRVFERVANILHDDCAFLSAFGDVSKPERYSGDNIIYKPPGHSAPDMVYLGAMTNFDVTYNWIQDKCVPLVREITFENGEELTEEGLPFLILFHMKEDTESLEIFQNEVARQLISEKGTINFLHADCDKFRHPLLHIQKTPADCPVIAIDSFRHMYVFGDFKDVLIPGKLKQFVFDLHSGKLHREFHHGPDPTDTAPGEQAQDVASSPPESSFQKLAPSEYRYTLLRDRDEL","proteinLength":"Full Length","predictedMolecularWeight":"48 kDa","actualMolecularWeight":null,"aminoAcidEnd":406,"aminoAcidStart":30,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BS26","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TXNDC4 also known as ERp46 or thioredoxin domain-containing protein 4 is an enzyme involved in protein folding within the endoplasmic reticulum. With a molecular mass of approximately 47 kDa this protein exhibits oxidoreductase activity and facilitates the formation and isomerization of disulfide bonds in nascent proteins. TXNDC4 is widely expressed across various tissues with a higher presence in the liver and pancreas reflecting its significant role in protein maturation and quality control processes.
Biological function summary

TXNDC4 influences various cellular mechanisms by maintaining redox homeostasis and promoting the correct folding of proteins. It functions as part of a multi-protein complex interacting with other chaperones and co-chaperones within the endoplasmic reticulum. These interactions are critical for efficient protein maturation preventing the accumulation of misfolded proteins and thereby avoiding cellular stress. TXNDC4 is essential for maintaining the functional integrity of secretory pathways which support numerous cellular functions.

Pathways

TXNDC4 plays a role in the unfolded protein response (UPR) and the oxidative folding pathway. The protein interacts closely with components such as protein disulfide isomerase (PDI) and ER oxidoreductin 1 (Ero1) within these pathways. Together these proteins facilitate efficient protein folding and prevent overaccumulation of unfolded proteins. The activity of TXNDC4 also relates to the regulation of cellular redox conditions which are pivotal for maintaining cellular homeostasis and proper response to stress.

Research indicates a connection between TXNDC4 and several conditions such as cancer and diabetes. Abnormal expression or malfunction of TXNDC4 can contribute to improper protein folding which may lead to unfolded protein response activation enhancing cellular stress conditions associated with these disorders. In cancer interactions between TXNDC4 and other proteins like Ero1α can alter redox balance affecting cell proliferation and survival. In diabetes improper protein processing in insulin-secreting cells may involve TXNDC4 dysfunction impacting insulin secretion and cellular homeostasis.
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Specifications

Form

Liquid

Additional notes

ab99218 was purified using conventional chromatography techniques.

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General info

Function

Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif (PubMed : 11847130, PubMed : 14517240). Inhibits the calcium channel activity of ITPR1 (PubMed : 15652484). May have a role in the control of oxidative protein folding in the endoplasmic reticulum (PubMed : 11847130, PubMed : 14517240, PubMed : 29858230). Required to retain ERO1A and ERO1B in the endoplasmic reticulum (PubMed : 11847130, PubMed : 29858230).

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Product protocols

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Target data

Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif (PubMed : 11847130, PubMed : 14517240). Inhibits the calcium channel activity of ITPR1 (PubMed : 15652484). May have a role in the control of oxidative protein folding in the endoplasmic reticulum (PubMed : 11847130, PubMed : 14517240, PubMed : 29858230). Required to retain ERO1A and ERO1B in the endoplasmic reticulum (PubMed : 11847130, PubMed : 29858230).
See full target information ERP44
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