Recombinant Human TXNIP protein is a Human Full Length protein, in the 1 to 391 aa range, expressed in Cell free, with >=90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Cell free
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M V M F K K I K S F E V V F N D P E K V Y G S G E K V A G R V I V E V C E V T R V K A V R I L A C G V A K V L W M Q G S Q Q C K Q T S E Y L R Y E D T L L L E D Q P T G E N E M V I M R P G N K Y E Y K F G F E L P Q G P L G T S F K G K Y G C V D Y W V K A F L D R P S Q P T Q E T K K N F E V V D L V D V N T P D L M A P V S A K K E K K V S C M F I P D G R V S V S A R I D R K G F C E G D E I S I H A D F E N T C S R I V V P K A A I V A R H T Y L A N G Q T K V L T Q K L S S V R G N H I I S G T C A S W R G K S L R V Q K I R P S I L G C N I L R V E Y S L L I Y V S V P G S K K V I L D L P L V I G S R S G L S S R T S S M A S R T S S E M S W V D L N I P D T P E A P P C Y M D V I P E D H R L E S P T T P L L D D M D G S Q D S P I F M Y A P E F K F M P P P T Y T E V D P C I L N N N V Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability (PubMed:17603038). Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest (PubMed:12821938). Required for the maturation of natural killer cells (By similarity). Acts as a suppressor of tumor cell growth (PubMed:18541147). Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (PubMed:21460850).
Alternative names
VDUP1, TXNIP, Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1
Recommended products
Recombinant Human TXNIP protein is a Human Full Length protein, in the 1 to 391 aa range, expressed in Cell free, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Cell free
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9H3M7-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% 2-Octadecoxyethanol
Sequence info
Amino acid sequence
- M V M F K K I K S F E V V F N D P E K V Y G S G E K V A G R V I V E V C E V T R V K A V R I L A C G V A K V L W M Q G S Q Q C K Q T S E Y L R Y E D T L L L E D Q P T G E N E M V I M R P G N K Y E Y K F G F E L P Q G P L G T S F K G K Y G C V D Y W V K A F L D R P S Q P T Q E T K K N F E V V D L V D V N T P D L M A P V S A K K E K K V S C M F I P D G R V S V S A R I D R K G F C E G D E I S I H A D F E N T C S R I V V P K A A I V A R H T Y L A N G Q T K V L T Q K L S S V R G N H I I S G T C A S W R G K S L R V Q K I R P S I L G C N I L R V E Y S L L I Y V S V P G S K K V I L D L P L V I G S R S G L S S R T S S M A S R T S S E M S W V D L N I P D T P E A P P C Y M D V I P E D H R L E S P T T P L L D D M D G S Q D S P I F M Y A P E F K F M P P P T Y T E V D P C I L N N N V Q
- Accession
- Q9H3M7
- Protein length
- Full Length
- Predicted molecular weight
- 57.7 kDa
- Amino acids
- 1 to 391
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability (PubMed:17603038). Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest (PubMed:12821938). Required for the maturation of natural killer cells (By similarity). Acts as a suppressor of tumor cell growth (PubMed:18541147). Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (PubMed:21460850).
- Sequence similarities
Belongs to the arrestin family.
- Post-translational modifications
Ubiquitinated; undergoes heterotypic 'Lys-48'-/'Lys-63'-branched polyubiquitination catalyzed by ITCH and UBR5 resulting in proteasomal degradation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TXNIP also known as thioredoxin-interacting protein or VDUP1 is an inhibitory protein that binds to and regulates the activity of thioredoxin. It has a molecular mass of around 46 kDa. TXNIP is expressed in various tissues including heart liver and skeletal muscle. It acts to control intracellular redox states by modulating thioredoxin’s antioxidant functions. TXNIP expression can vary according to oxidative stress and cellular metabolic conditions.
- Biological function summary
TXNIP regulates cellular processes such as glucose metabolism stress response and apoptosis. It does not form a complex but functions by negatively regulating the activity of thioredoxin. This protein can lead to increased reactive oxygen species (ROS) when it inhibits thioredoxin influencing cellular redox balance. TXNIP therefore plays a regulatory role in metabolic pathways and cellular stress responses by modulating thioredoxin activity and ROS levels.
- Pathways
TXNIP is a significant component of the oxidative stress and glucose metabolism pathways. In the oxidative stress pathway TXNIP interacts with thioredoxin influencing ROS detoxification and redox-sensitive signaling. In glucose metabolism TXNIP can affect insulin signaling by interacting with glucose transporter-1 (GLUT1). These interactions highlight TXNIP's regulatory role in cellular metabolism and stress-related signaling making it a node of convergence with proteins like thioredoxin and GLUT1.
- Associated diseases and disorders
TXNIP levels have been implicated in conditions such as type 2 diabetes and cardiovascular diseases. Elevated TXNIP expression has been observed in insulin resistance where it affects glucose uptake by modifying GLUT1 activity and links to thioredoxin in cardiovascular tissues resulting in oxidative stress damage. These connections position TXNIP as a potential therapeutic target for managing metabolic disorders and cardiovascular complications.
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1 product image
SDS-PAGE - Recombinant Human TXNIP protein (ab289756)
SDS-PAGE analysis of ab289756
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