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AB204206

Recombinant Human TXNRD1 protein

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(1 Publication)

Recombinant Human TXNRD1 protein is a Human Full Length protein, in the 2 to 499 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

GRIM12, KDRF, TXNRD1, TR, Gene associated with retinoic and interferon-induced mortality 12 protein, KM-102-derived reductase-like factor, Peroxidase TXNRD1, Thioredoxin reductase TR1, GRIM-12, Gene associated with retinoic and IFN-induced mortality 12 protein

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

No

Accession

Q16881

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>1. May be used as auto-antibodies detection reagent, which will react with sera of some auto-immuno-diseases and cancer patients.</p><p>2. May be used for in vitro human TXNRD1 mediated oxidative stress regulation study.</p><p>3. May be used for TXNRD1 protein-protein interaction assay.</p><p>4. May be used as specific substrate protein for kinase, and ubiquitin (Sumo pathway) related enzyme functional screening assays.</p><p>5. As antigen for specific antibody production.</p>" } } }
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Sequence info

[{"sequence":"MASMTGGQQMGRGHHHHHHENLYFQGGEFNGPEDLPKSYDYDLIIIGGGSGGLAAAKEAAQYGK\r\nKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHDWDR\r\nMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKIYSAERFLIATGER\r\nPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGF\r\nDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIG\r\nRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQR\r\nLYAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRDNNK\r\nCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSV\r\nTKRSGASILQAGCUG","proteinLength":"Full Length","predictedMolecularWeight":"57.78 kDa","actualMolecularWeight":null,"aminoAcidEnd":499,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q16881","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin reductase 1 (TXNRD1) also known as TRXR1 is a selenoprotein that plays an important role in maintaining cellular redox balance by catalyzing the reduction of thioredoxin (TRX). It possesses a molecular mass of approximately 55 kDa. TXNRD1 can be found in many tissues displaying a stronger presence in the liver kidney and pancreas indicating its significance in these organs. The enzyme contains a selenocysteine residue at its active site which is essential for its enzymatic activity.
Biological function summary

TXNRD1 functions as an integral part of the thioredoxin system which includes thioredoxin and NADPH. It maintains the redox homeostasis in cells by regenerating oxidized thioredoxin. This action allows thioredoxin to in turn reduce disulfide bonds in various substrate proteins. Furthermore TXNRD1 participates in regulating cellular processes such as DNA synthesis and repair signal transduction and antioxidant defense. It does not work alone but collaborates with various redox-active components to achieve its roles.

Pathways

TXNRD1 participates in the antioxidant defense and redox signaling pathways. Within the antioxidant defense pathway TXNRD1 along with thioredoxin provides resistance to oxidative stress. In the redox signaling pathway the protein interacts with NF-κB signaling processes impacting cell inflammation and apoptosis. These pathways are vital for cellular health and are connected through thioredoxin emphasizing the interconnected nature of TXNRD1-related processes.

TXNRD1 has associations with cancer and neurodegenerative diseases. Elevated levels of TXNRD1 have been observed in various cancers including lung and hepatocellular carcinoma suggesting a role in tumor growth and survival through redox regulation. Additionally TXNRD1 is linked to neurodegenerative diseases such as Alzheimer's where oxidative stress plays a contributory role. In these conditions TXNRD1 interacts closely with other redox proteins like glutathione reductase highlighting its potential as a therapeutic target.
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Specifications

Form

Liquid

Additional notes

refolded and chromatographically purified.

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General info

Function

Reduces disulfide protein thioredoxin (Trx) to its dithiol-containing form (PubMed : 8577704). Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. A selenocysteine residue at the C-terminal active site is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2) (PubMed : 10849437).. Isoform 1. Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions.. Isoform 4. Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2.. Isoform 5. Enhances the transcriptional activity of the estrogen receptor ESR2 only (PubMed : 15199063). Mediates cell death induced by a combination of interferon-beta and retinoic acid (PubMed : 9774665).

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Post-translational modifications

Isoform 5. The N-terminus is blocked.. ISGylated.

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Product protocols

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Target data

Reduces disulfide protein thioredoxin (Trx) to its dithiol-containing form (PubMed : 8577704). Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. A selenocysteine residue at the C-terminal active site is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2) (PubMed : 10849437).. Isoform 1. Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions.. Isoform 4. Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2.. Isoform 5. Enhances the transcriptional activity of the estrogen receptor ESR2 only (PubMed : 15199063). Mediates cell death induced by a combination of interferon-beta and retinoic acid (PubMed : 9774665).
See full target information TXNRD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 24:101153 PubMed30909158

2019

Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase.

Applications

Unspecified application

Species

Unspecified reactive species

Xinzhi Wang,Ming Gao,Jiyun Zhang,Ying Ma,Wenshu Qu,Jingyu Liang,Hao Wu,Hongmei Wen
View all publications
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